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Abstract

Actinomycete strain ZLN234 was isolated from a frozen soil sample, which was collected from a glacier front in the Arctic. Chemotaxonomic and morphological characteristics were found to be typical of members of the genus . The strain formed a distinct phyletic line in the 16S rRNA gene tree and was closely related to ATCC 3336 (98.63 % similarity) and B7319 (98.56 %). Phylogenetic analysis based on the B gene also showed clearly that strain ZLN234 was different from all recognized species. The whole-cell sugars were galactose, glucose, ribose and rhamnose. The whole-cell-wall amino acids included alanine, glutamine, glycine and -diaminopimelic acid with a minor amount of -diaminopimelic acid. The characteristic polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and unknown phospholipids. The major menaquinones were MK-9(H), MK-9(H) and MK-9(H). The major cellular fatty acids comprised anteiso-C, iso-C and C with a small amount of iso-Cω7 and anteiso-C. The DNA G+C content of strain ZLN234 was 77.5 mol%. On the basis of these phenotypic, phylogenetic and chemotaxonomic characteristics, strain ZLN234 ( = CCTCC AA 2015005 = DSM 100713) is the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

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2016-03-01
2021-08-01
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