RT Journal Article SR Electronic(1) A1 Guo, Siyu A1 Liu, Chongxi A1 Liu, Shuanghe A1 Guan, Xuejiao A1 Guo, Lifeng A1 Jia, Feiyu A1 Wang, Xiangjing A1 Xiang, WenshengYR 2016 T1 Streptomyces polygonati sp. nov., an endophytic actinomycete isolated from a root of Polygonatum odoratum (Mill.) JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 3 SP 1488 OP 1493 DO https://doi.org/10.1099/ijsem.0.000906 PB Microbiology Society, SN 1466-5034, AB A novel actinomycete, designated strain NEAU-G9T, was isolated from the root of Polygonatum odoratum (Mill.) collected from Harbin, Heilongjiang province, north China, and was characterized using a polyphasic approach. Key morphological and chemotaxonomic properties confirmed the affiliation of strain NEAU-G9T to the genus Streptomyces. Strain NEAU-G9T contained ll-diaminopimelic acid as the diamino acid. The predominant menaquinones were MK-9(H8), MK-9(H6) and MK-9(H4). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The predominant fatty acids were iso-C16 : 0, anteiso-C15 : 0 and C16 : 0.16S rRNA gene sequence similarity studies showed that strain NEAU-G9T belongs to the genus Streptomyces and exhibits the highest sequence similarity to Streptomyces yanglinensis JCM 13275T (97.75 %). However, phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-G9T is most closely related to Streptomyces misakiensis JCM 4062T (97.12 % sequence similarity). A combination of DNA–DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-G9T can be clearly distinguished from S. yanglinensis JCM 13275T and S. misakiensis JCM 4062T. Consequently, strain NEAU-G9T represents a novel species of the genus Streptomyces, for which the name Streptomyces polygonati sp. nov. is proposed. The type strain is NEAU-G9T ( = CGMCC 4.7237T = DSM 100521T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000906