1887

Abstract

Two isolates, designated B15.09-116 and B15.09-124, were recovered from bottled mineral water in Portugal. Based on 16S rRNA gene sequence analysis, these strains were related most closely to species of the genus (belonging to the family ) with pairwise sequence similarities of 93.0–93.6 %. The isolates were not pigmented and formed Gram-stain-negative, short, motile rod-shaped cells. The organisms were strictly aerobic, oxidase-positive and catalase-negative. These organisms also fixed N. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. Ubiquinone 8 was the major respiratory quinone. The DNA G+C content of strain B15.09-116 was 49.8 mol%. Based on phylogenetic, physiological and biochemical characteristics the two strains are considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is B15.09-116 ( = CECT 8581 = LMG 28208).

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2016-03-01
2020-01-21
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References

  1. Albuquerque L., França L., Taborda M., La Cono V., Yakimov M., da Costa M. S.. 2015; Palleronia abyssalis sp. nov., isolated from the deep Mediterranean Sea and the emended description of the genus Palleronia and of the species Palleronia marisminoris. Antonie van Leeuwenhoek107:633–642 [CrossRef][PubMed]
    [Google Scholar]
  2. Beringer J. E.. 1974; R factor transfer in Rhizobium leguminosarum. J Gen Microbiol84:188–198[PubMed]
    [Google Scholar]
  3. Bradford M. M.. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem72:248–254 [CrossRef][PubMed]
    [Google Scholar]
  4. Castanheira N., Dourado A. C., Alves P. I., Cortés-Pallero A. M., Delgado-Rodríguez A. I., Prazeres Â., Borges N., Sánchez C., Barreto Crespo M. T., Fareleira P.. 2014; Annual ryegrass-associated bacteria with potential for plant growth promotion. Microbiol Res169:768–779 [CrossRef][PubMed]
    [Google Scholar]
  5. Castellani A., Chalmers A.. 1919; Manual of Tropical Medicine, 3rd edn. New York: Williams, Wood and Co; [CrossRef]
    [Google Scholar]
  6. Chen W.-M., Huang W.-C., Sheu S.-Y.. 2013; Derxia lacustris sp. nov., a nitrogen-fixing bacterium isolated from a freshwater lake. Int J Syst Evol Microbiol63:965–970 [CrossRef][PubMed]
    [Google Scholar]
  7. Coenye T., Vanlaere E., Samyn E., Falsen E., Larsson P., Vandamme P.. 2005; Advenella incenata gen. nov., sp. nov., a novel member of the Alcaligenaceae, isolated from various clinical samples. Int J Syst Evol Microbiol55:251–256 [CrossRef][PubMed]
    [Google Scholar]
  8. da Costa M. S., Albuquerque L., Nobre M. F., Wait R.. 2011a; The identification of polar lipids in prokaryotes. Methods Microbiol38:165–181 [CrossRef]
    [Google Scholar]
  9. da Costa M. S., Albuquerque L., Nobre M. F., Wait R.. 2011b; The extraction and identification of respiratory lipoquinones of prokaryotes and their use in taxonomy. Methods Microbiol38:197–206 [CrossRef]
    [Google Scholar]
  10. da Costa M. S., Albuquerque L., Nobre M. F., Wait R.. 2011c; The identification of fatty acids in bacteria. Methods Microbiol38:183–196 [CrossRef]
    [Google Scholar]
  11. França L., Lopéz-Lopéz A., Rosselló-Móra R., da Costa M. S.. 2015; Microbial diversity and dynamics of a groundwater and a still bottled natural mineral water. Environ Microbiol17:577–593 [CrossRef][PubMed]
    [Google Scholar]
  12. Jensen H. L., Petersen E. J., De P. K., Bhattacharya R.. 1960; A new nitrogen-fixing bacterium: Derxia gummosa nov. gen. nov. spec. Arch Mikrobiol36:182–195 [CrossRef][PubMed]
    [Google Scholar]
  13. Kämpfer P., Denger K., Cook A. M., Lee S.-T., Jäckel U., Denner E. B., Busse H.-J.. 2006; Castellaniella gen. nov., to accommodate the phylogenetic lineage of Alcaligenes defragrans, and proposal of Castellaniella defragrans gen. nov., comb. nov. and Castellaniella denitrificans sp. nov. Int J Syst Evol Microbiol56:815–819 [CrossRef][PubMed]
    [Google Scholar]
  14. Kim Y.-J., Kim M. K., Im W.-T., Srinivasan S., Yang D.-C.. 2010; Parapusillimonas granuli gen. nov., sp. nov., isolated from granules from a wastewater-treatment bioreactor. Int J Syst Evol Microbiol60:1401–1406 [CrossRef][PubMed]
    [Google Scholar]
  15. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S., other authors. 2004; arb: a software environment for sequence data. Nucleic Acids Res32:1363–1371 [CrossRef][PubMed]
    [Google Scholar]
  16. Mascarua-Esparza M. A., Villa-Gonzalez R., Caballero-Mellado J.. 1988; Acetylene reduction and indoleacetic acid production by Azospirillum isolates from Cactaceous plants. Plant Soil106:91–95 [CrossRef]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W.. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39:159–167 [CrossRef]
    [Google Scholar]
  18. Moreno-Lopez M.. 1952; El género Bordetella. Microbiol Esp5:177–181
    [Google Scholar]
  19. Nielsen P., Fritze D., Priest F. G.. 1995; Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology141:1745–1761 [CrossRef]
    [Google Scholar]
  20. Poly F., Monrozier L. J., Bally R.. 2001; Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil. Res Microbiol152:95–103 [CrossRef][PubMed]
    [Google Scholar]
  21. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E.. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol46:1088–1092 [CrossRef][PubMed]
    [Google Scholar]
  22. Reasoner D. J., Geldreich E. E.. 1985; A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol49:1–7[PubMed]
    [Google Scholar]
  23. Reinhold B., Hurek T., Niemann E.-G., Fendrik I.. 1986; Close association of Azospirillum and diazotrophic rods with different root zones of kallar grass. Appl Environ Microbiol52:520–526[PubMed]
    [Google Scholar]
  24. Smibert R., Krieg N.. 1981; General characterization. In Manual of Methods for General Bacteriology pp409–443Edited by Gerhardt P., Murray R., Costilow R., Nester E., Wood W., Krieg N., Phillips G.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  25. Stolz A., Bürger S., Kuhm A., Kämpfer P., Busse H.-J.. 2005; Pusillimonas noertemannii gen. nov., sp. nov., a new member of the family Alcaligenaceae that degrades substituted salicylates. Int J Syst Evol Microbiol55:1077–1081 [CrossRef][PubMed]
    [Google Scholar]
  26. Sugimoto C., Isayama Y., Sakazaki R., Kuramochi S.. 1983; Transfer of Haemophilus equigenitalis Taylor et al. 1978 to the genus Taylorella gen. nov. as Taylorella equigenitalis comb. nov. Curr Microbiol9:155–162 [CrossRef]
    [Google Scholar]
  27. Vandamme P., Segers P., Ryll M., Hommez J., Vancanneyt M., Coopman R., De Baere R., Van de Peer Y., Kersters K., other authors. 1998; Pelistega europaea gen. nov., sp. nov., a bacterium associated with respiratory disease in pigeons: taxonomic structure and phylogenetic allocation. Int J Syst Bacteriol48:431–440 [CrossRef][PubMed]
    [Google Scholar]
  28. Vaz-Moreira I., Figueira V., Lopes A. R., De Brandt E., Vandamme P., Nunes O. C., Manaia C. M.. 2011; Candidimonas nitroreducens gen. nov., sp. nov. and Candidimonas humi sp. nov., isolated from sewage sludge compost. Int J Syst Evol Microbiol61:2238–2246 [CrossRef][PubMed]
    [Google Scholar]
  29. Wiedmann-al-Ahmad M., Tichy H. V., Schön G.. 1994; Characterization of Acinetobacter type strains and isolates obtained from wastewater treatment plants by PCR fingerprinting. Appl Environ Microbiol60:4066–4071[PubMed]
    [Google Scholar]
  30. Willems A., Gilhaus H., Beer W., Mietke H., Gelderblom H. R., Burghardt B., Voigt W., Reissbrodt R.. 2002; Brackiella oedipodis gen. nov., sp. nov., gram-negative, oxidase-positive rods that cause endocarditis of cotton-topped tamarin (Saguinus oedipus). Int J Syst Evol Microbiol52:179–186 [CrossRef][PubMed]
    [Google Scholar]
  31. Yabuuchi E., Yano I.. 1981; Achromobacter gen. nov. and Achromobacter xylosoxidans (ex Yabuuchi and Ohyama 1971) nom. rev. Int J Syst Bacteriol31:477–478 [CrossRef]
    [Google Scholar]
  32. Yarza P., Ludwig W., Euzéby J., Amann R., Schleifer K.-H., Glöckner F. O., Rosselló-Móra R.. 2010; Update of the All-Species Living Tree Project based on 16S rRNA and 23S rRNA sequence analyses. Syst Appl Microbiol33:291–299 [CrossRef][PubMed]
    [Google Scholar]
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