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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 66, Issue 3

Description of sp. nov., sp. nov. and gen. nov., sp. nov., isolated from mesophilic laboratory-scale biogas reactors, and emended description of the genus Free

Abstract

Three novel, facultatively anaerobic bacteria of the family (phylum ) were isolated from mesophilic laboratory-scale biogas reactors. The strains were Gram-negative rods. Optimal growth occurred between 35 and 45 °C and at pH 7.1–7.8. The main fermentation products were acetic and propionic acids. The predominant fatty acid in all strains was anteiso-C, and the only respiratory quinone detected was menaquinone MK-8. 16S rRNA gene sequence comparison indicated that strains M3/6 and ING2-E5B were most closely related to the type strain of , with sequence similarities of 97.3 and 94.5 %. Strain ING2-E5A showed the closest affiliation to the type strain of , with 97 % sequence identity. DNA–DNA hybridization of strain M3/6 and ING2-E5A with the most closely related type strains showed 43.3–45.6 and 23.8–25.7 % relatedness, respectively, which supports the conclusion that both isolates represent novel species. Phylogenetic analysis and comparison of cellular fatty acid patterns indicated that strain ING2-E5B cannot be classified as a member of any previously described genus. Therefore, because of the physiological, genotypic and chemotaxonomic differences, it is proposed to designate novel species within the genera and , sp. nov. (type strain M3/6 = DSM 28694 = CECT 8610 = LMG 28299) and sp. nov. (type strain ING2-E5A = DSM 28695 = CECT 8611), and a novel species of a new genus, gen. nov., sp. nov. (type strain of is ING2-E5B = DSM 28696 = CECT 8609 = LMG 28429). In addition, an emended description of the genus is provided.

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© 2016 IUMS

Erratum

An erratum has been published for this content:
Erratum to Description of sp. nov., sp. nov. and gen. nov., sp. nov., isolated from mesophilic laboratory-scale biogas reactors, and emended description of the genus Proteiniphilum
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2016-03-01
2024-04-19
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Description of Proteiniphilum saccharofermentans sp. nov., Petrimonas mucosa sp. nov. and Fermentimonas caenicola gen. nov., sp. nov., isolated from mesophilic laboratory-scale biogas reactors, and emended description of the genus Proteiniphilum
Int J Syst Evol Microbiol 66, 1466 (2016); https://doi.org/10.1099/ijsem.0.000902
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