A Gram-staining-negative, strictly aerobic, rod-shaped and bright-yellow-pigmented bacterium, motile by means of a single polar flagellum, designated THG-MD21T, was isolated from rhizosphere soil of Radix ophiopogonis in Henan province, PR China. On the basis of 16S rRNA gene sequence similarity, strain THG-MD21T belongs to the genus Luteimonas and was most closely related to Luteimonas aestuarii B9T (98.2 % sequence similarity), Lysobacter panaciterrae Gsoil 068T (97.2 %) and Luteimonas marina FR1330T (97.0 %). The DNA G+C content was 64.4 mol%. The DNA–DNA relatedness between strain THG-MD21T and its closest phylogenetic neighbours was below 30.0 %. The only isoprenoid quinone detected in strain THG-MD21T was ubiquinone-8.The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, and the predominant fatty acids were iso-C11 : 0, iso-C15 : 0, iso-C16 : 0, C16 : 0 and iso-C17 : 1ω9c. The DNA–DNA hybridization result and characteristics revealed by a polyphasic study showed that strain THG-MD21T represents a novel species, for which the name Luteimonas terrae sp. nov. is proposed. The type strain is THG-MD21T ( = KACC 18131T = JCM 30122T).
CollinsM. D., JonesD.1981; Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 45:316–354[PubMed]
EzakiT., HashimotoY., YabuuchiE.1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
FrankJ. A., ReichC. I., SharmaS., WeisbaumJ. S., WilsonB. A., OlsenG. J.2008; Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. Appl Environ Microbiol 74:2461–2470 [View Article][PubMed]
GillisM., De LeyJ., De CleeneM.1970; The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur J Biochem 12:143–153 [View Article][PubMed]
HallT. A.1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
LipskiA., StackebrandtE.2005; Genus III. Luteimonas Finkmann, Altendorf, Stackebrandt and Lipski 2000, 280VP
. In Bergey's Manual of Systematic Bacteriology, 2nd edn. vol. 2 pp 93–94Edited byBrennerD. J., KriegN. R., StaleyJ. T., GarrityG. M. New York: Springer;
MesbahM., PremachandranU., WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
MinnikinD. E., PatelP. V., AlshamaonyL., GoodfellowM.1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [View Article]
MinnikinD. E., O'DonnellA. G., GoodfellowM., AldersonG., AthalyeM., SchaalA., ParleetJ. H.1984; An integrated procedure for the extraction of bacterial isoprenoid quinines and polar lipids. J Microbiol Methods 2:233–241 [View Article]
MooreD. D., DowhanD.1995; Preparation and analysis of DNA. In Current Protocols in Molecular Biology pp 2–11Edited byAusubelF. W., BrentR., KingstonR. E., MooreD. D., SeidmanJ. G., SmithJ. A., StruhlK. New York: Wiley;
StackebrandtE., GoebelB. M.1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
TenL. N., JungH. M., ImW. T., YooS. A., OhH. M., LeeS. T.2009; Lysobacter panaciterrae sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 59:958–963 [View Article][PubMed]
ThompsonJ. D., GibsonT. J., PlewniakF., JeanmouginF., HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
WayneL. G., BrennerD. J., ColwellR. R., GrimontP.A.D., KandlerO., KrichevskyM. I., MooreL. H., MooreW.E.C., MurrayR.G.E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]