1887

Abstract

A Gram-staining-negative, strictly aerobic, rod-shaped and bright-yellow-pigmented bacterium, motile by means of a single polar flagellum, designated THG-MD21, was isolated from rhizosphere soil of in Henan province, PR China. On the basis of 16S rRNA gene sequence similarity, strain THG-MD21 belongs to the genus and was most closely related to B9 (98.2 % sequence similarity), Gsoil 068 (97.2 %) and FR1330 (97.0 %). The DNA G+C content was 64.4 mol%. The DNA–DNA relatedness between strain THG-MD21 and its closest phylogenetic neighbours was below 30.0 %. The only isoprenoid quinone detected in strain THG-MD21 was ubiquinone-8.The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, and the predominant fatty acids were iso-C, iso-C, iso-C, C and iso-Cω9. The DNA–DNA hybridization result and characteristics revealed by a polyphasic study showed that strain THG-MD21 represents a novel species, for which the name sp. nov. is proposed. The type strain is THG-MD21 ( = KACC 18131 = JCM 30122).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000901
2016-05-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/5/1920.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000901&mimeType=html&fmt=ahah

References

  1. Baik K. S., Park S. C., Kim M. S., Kim E. M., Park C., Chun J., Seong C. N.. ( 2008;). Luteimonas marina sp. nov., isolated from seawater. Int J Syst Evol Microbiol 58: 2904–2908 [CrossRef] [PubMed].
    [Google Scholar]
  2. Chou J. H., Cho N. T., Arun A. B., Young C. C., Chen W. M.. ( 2008;). Luteimonas aquatica sp. nov., isolated from fresh water from Southern Taiwan. Int J Syst Evol Microbiol 58: 2051–2055 [CrossRef] [PubMed].
    [Google Scholar]
  3. Collins M. D., Jones D.. ( 1981;). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 45: 316–354 [PubMed].
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39: 224–229 [CrossRef].
    [Google Scholar]
  5. Fan X., Yu T., Li Z., Zhang X. H.. ( 2014;). Luteimonas abyssi sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol 64: 668–674 [CrossRef] [PubMed].
    [Google Scholar]
  6. Felsenstein J.. ( 1985;). Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  7. Finkmann W., Altendorf K., Stackebrandt E., Lipski A.. ( 2000;). Characterization of N2O-producing Xanthomonas-like isolates from biofilters as Stenotrophomonas nitritireducens sp. nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov. Int J Syst Evol Microbiol 50: 273–282 [CrossRef] [PubMed].
    [Google Scholar]
  8. Frank J. A., Reich C. I., Sharma S., Weisbaum J. S., Wilson B. A., Olsen G. J.. ( 2008;). Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. Appl Environ Microbiol 74: 2461–2470 [CrossRef] [PubMed].
    [Google Scholar]
  9. Gillis M., De Ley J., De Cleene M.. ( 1970;). The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur J Biochem 12: 143–153 [CrossRef] [PubMed].
    [Google Scholar]
  10. Hall T. A.. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41: 95–98.
    [Google Scholar]
  11. Hiraishi A., Ueda Y., Ishihara J., Mori T.. ( 1996;). Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42: 457–469 [CrossRef].
    [Google Scholar]
  12. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  13. Kimura M.. ( 1983;). The Neutral Theory of Molecular Evolution Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  14. Lipski A., Stackebrandt E.. ( 2005;). Genus III. Luteimonas Finkmann, Altendorf, Stackebrandt and Lipski 2000, 280VP. . In Bergey's Manual of Systematic Bacteriology, 2nd edn.vol. 2, pp. 93–94. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M.. New York: Springer;.
    [Google Scholar]
  15. McConaughy B. L., Laird C. D., McCarthy B. J.. ( 1969;). Nucleic acid reassociation in formamide. Biochemistry 8: 3289–3295 [CrossRef] [PubMed].
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  17. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M.. ( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27: 104–117 [CrossRef].
    [Google Scholar]
  18. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parleet J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinines and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  19. Moore D. D., Dowhan D.. ( 1995;). Preparation and analysis of DNA. . In Current Protocols in Molecular Biology, pp. 2–11. Edited by Ausubel F. W., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K.. New York: Wiley;.
    [Google Scholar]
  20. Park Y. J., Park M. S., Lee S. H., Park W., Lee K., Jeon C. O.. ( 2011;). Luteimonas lutimaris sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 61: 2729–2733 [CrossRef] [PubMed].
    [Google Scholar]
  21. Roh S. W., Kim K. H., Nam Y. D., Chang H. W., Kim M. S., Yoon J. H., Oh H. M., Bae J. W.. ( 2008;). Luteimonas aestuarii sp. nov., isolated from tidal flat sediment. J Microbiol 46: 525–529 [CrossRef] [PubMed].
    [Google Scholar]
  22. Romanenko L. A., Tanaka N., Svetashev V. I., Kurilenko V. V., Mikhailov V. V.. ( 2013;). Luteimonas vadosa sp. nov., isolated from seashore sediment. Int J Syst Evol Microbiol 63: 1261–1266 [CrossRef] [PubMed].
    [Google Scholar]
  23. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425.
    [Google Scholar]
  24. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  25. Stabili L., Gravili C., Tredici S. M., Piraino S., Talà A., Boero F., Alifano P.. ( 2008;). Epibiotic Vibrio luminous bacteria isolated from some hydrozoa and bryozoa species. Microb Ecol 56: 625–636 [CrossRef] [PubMed].
    [Google Scholar]
  26. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44: 846–849 [CrossRef].
    [Google Scholar]
  27. Sun Z. B., Zhang H., Yuan X. F., Wang Y. X., Feng D. M., Wang Y. H., Feng Y. J.. ( 2012;). Luteimonas cucumeris sp. nov., isolated a from cucumber leaf. Int J Syst Evol Microbiol 62: 2916–2920 [CrossRef] [PubMed].
    [Google Scholar]
  28. Tamaoka J., Katayama-Fujiruma Y., Kuraishi H.. ( 1983;). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54: 31–36 [CrossRef].
    [Google Scholar]
  29. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  30. Ten L. N., Jung H. M., Im W. T., Yoo S. A., Oh H. M., Lee S. T.. ( 2009;). Lysobacter panaciterrae sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 59: 958–963 [CrossRef] [PubMed].
    [Google Scholar]
  31. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882 [CrossRef] [PubMed].
    [Google Scholar]
  32. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P.A.D., Kandler O., Krichevsky M. I., Moore L. H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463–464 [CrossRef].
    [Google Scholar]
  33. Wu G., Liu Y., Li Q., Du H., You J., Li H., Ke C., Zhang X., Yu J., Zhao T.. ( 2013;). Luteimonas huabeiensis sp. nov., isolated from stratum water. Int J Syst Evol Microbiol 63: 3352–3357 [CrossRef] [PubMed].
    [Google Scholar]
  34. Xin Y., Cao X., Wu P., Xue S.. ( 2014;). Luteimonas dalianensis sp. nov., an obligate marine bacterium isolated from seawater. J Microbiol 52: 729–733 [CrossRef] [PubMed].
    [Google Scholar]
  35. Young C. C., Kämpfer P., Chen W. M., Yen W. S., Arun A. B., Lai W. A., Shen F. T., Rekha P. D., Lin K. Y., Chou J. H.. ( 2007;). Luteimonas composti sp. nov., a moderately thermophilic bacterium isolated from food waste. Int J Syst Evol Microbiol 57: 741–744 [CrossRef] [PubMed].
    [Google Scholar]
  36. Zhang D. C., Liu H. C., Xin Y. H., Zhou Y. G., Schinner F., Margesin R.. ( 2010;). Luteimonas terricola sp. nov., a psychrophilic bacterium isolated from soil. Int J Syst Evol Microbiol 60: 1581–1584 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000901
Loading
/content/journal/ijsem/10.1099/ijsem.0.000901
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error