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Abstract

Six strains of anaerobic bacteria, C13EG70, C13EG118, C13EG186, C13GAMG5, C13GAMG28 and C13GAMG40, were isolated from the caecum of a healthy chicken bred in Bogor, Indonesia. Phylogenetic analysis showed the isolates were separated into two groups. Group I (C13EG70 and C13EG118) showed nearly identical 16S rRNA gene sequences (99.9 % sequence similarity). Group II (C13EG186, C13GAMG5, C13GAMG28 and C13GAMG40) showed nearly identical 16S rRNA gene sequences (>99.4 % sequence similarity). The isolates showed low 16S rRNA gene sequence similarities to recognized species of the genus . High gene sequence similarities were found between type strains (C13EG70 and C13EG186) and JCM 13657 (87.9, 91.5 %, respectively). Physiological, biochemical and genotypic characteristics demonstrated that these strains could be separated from the type strain of . It is concluded that Group I and Group II represent novel species. Two novel species of the genus are proposed as sp. nov. (type strain C13EG70 = LIPI12-4-Ck732 = JSAT12-4-Ck732 = InaCC B449 = NBRC 110958) and sp. nov. (type strain C13EG186 = LIPI12-4-Ck844 = JSAT12-4-Ck884 = InaCC B451 = NBRC 110963).

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2016-03-01
2020-02-26
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