1887

Abstract

An obligatory anaerobic, Gram-stain-negative coccobacillus with black-pigmented colonies was isolated from the oral cavity of selected Australian marsupial species. Phenotypic and molecular criteria showed that this bacterium was a distinct species within the genus , and was closely related to and . This putative novel species and could be differentiated from by catalase activity. Further characterization by multi-locus enzyme electrophoresis of glutamate dehydrogenase and malate dehydrogenase enzyme mobility and matrix-assisted laser desorption ionization time-of-flight MS showed that this putative novel species could be differentiated phenotypically from and . Definitive identification by 16S rRNA gene sequencing showed that this bacterium belonged to a unique monophyletic lineage, phylogenetically distinct from (94.9 % similarity) and (95.5 %). This also was supported by 16S–23S rRNA intergenic spacer region and glutamate dehydrogenase gene sequencing. A new species epithet, sp. nov., is proposed for this bacterium, with DSM 28520 (=NCTC 13658=UQD444=MRK101), isolated from a musky rat kangaroo, as the type strain.

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2016-10-01
2021-11-28
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