1887

Abstract

A Gram-stain negative, aerobic, non-motile, short-rod-shaped bacterium, designated KBL-4-9, was isolated from the storage liquid in the stems of trees in Xinjiang, PR China. Strain KBL-4-9 grew at 4–45 °C (optimum 37 °C), 1–3 % (w/v) NaCl (optimum 1 %, w/v) and pH 5.5–9.5 (optimum pH 7.5). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain KBL-4-9 belonged to the genus and showed the highest 16S rRNA gene sequence similarity of 97.36 % to CL-AP6, followed by S3-3 (97.16 %), J64 (97.15 %) and C10-2 (96.47 %). Analysis of strain KBL-4-9 based on the three housekeeping genes, , and , further confirmed the phylogenetic assignment of the isolates. The DNA G+C content was 61.6 mol% ( = 2.19). DNA–DNA hybridization with CL-AP6, S3-3 and J64 revealed 49.3 % ( = 3.04), 41.2 % and 52.5 % ( = 4.45) relatedness, respectively. The major cellular fatty acids of strain KBL-4-9 were C, C, summed feature 8 (Cω7 and/or Cω6) and C cyclo. The major isoprenoid quinone was Q-9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain KBL-4-9 is considered to represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KBL-4-9 ( = JCM 19138 = CCTCC AB 2013069 = NRRL B-59988).

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2016-03-01
2019-10-18
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