1887

Abstract

A Gram-stain-negative, strictly aerobic, yellow, rod-shaped bacterium, designated strain HQQ, was isolated from a municipal wastewater treatment plant in Hebei Province, PR China. Comparative 16S rRNA gene sequence analyses showed that strain HQQ is a member of the genus and is closely related to ‘’ CCTCC AB 2014079 (94.8 %) and DSM 22219 (94.7 %). Phylogenetic analysis showed that strain HQQ clustered with JCM 18212 and KCTC 23915. The polar lipid profile of strain HQQ revealed the presence of phosphatidylethanolamine, six unknown aminolipids, one unknown glycolipid and one unknown lipid and the only isoprenoid quinone was MK-6. The dominant fatty acids of strain HQQ were iso-C, C and Cω7. The DNA G+C content of strain HQQ is 32 mol%. On the basis of the phylogenetic and phenotypic data, strain HQQ represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HQQ ( = CGMCC 1.15347 = KCTC 42935).

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2016-03-01
2019-09-15
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References

  1. Bergey D. H. , Harrison F. C. , Breed R. S. , Hammer B. W. , Huntoon F. M. E. . ( 1923;). Bergey's Manual of Determinative Bacteriology., Baltimore, MD: Williams & Wilkins;.
    [Google Scholar]
  2. Bernardet J. F. , Bowman J. , Dworkin M. , Falkow S. , Rosenberg E. , Schleifer K.-H. , Stackebrandt E. . ( 2006;). The genus Flavobacterium . . In The Prokaryotes: a Handbook on the Biology of Bacteria , 3rd edn.. vol. 7. pp. 481–531 New York: Springer; [CrossRef].
    [Google Scholar]
  3. Bernardet J. F. , Segers P. , Vancanneyt M. , Berthe F. , Kersters K. , Vandamme P. . ( 1996;). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46: 128–148 [CrossRef].
    [Google Scholar]
  4. Bernardet J. F. , Nakagawa Y. , Holmes B. , Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes, & Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes . ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52: 1049–1070 [PubMed].
    [Google Scholar]
  5. Chen W. M. , Huang W. C. , Young C. C. , Sheu S. Y. . ( 2013;). Flavobacterium tilapiae sp. nov., isolated from a freshwater pond, and emended descriptions of Flavobacterium defluvii and Flavobacterium johnsoniae . Int J Syst Evol Microbiol 63: 827–834 [CrossRef] [PubMed].
    [Google Scholar]
  6. Chun J. , Kang J. Y. , Jahng K. Y. . ( 2013;). Flavobacterium fontis sp. nov., isolated from freshwater. Int J Syst Evol Microbiol 63: 1653–1657 [CrossRef] [PubMed].
    [Google Scholar]
  7. Da Costa M. S. , Albuquerque L. , Nobre M. , Wait R. . ( 2011;). The identification of polar lipids in prokaryotes. Methods Microbiol 38: 165–181 [CrossRef].
    [Google Scholar]
  8. Dong K. , Xu B. , Zhu F. , Wang G. . ( 2013;). Flavobacterium hauense sp. nov., isolated from soil and emended descriptions of Flavobacterium subsaxonicum, Flavobacterium beibuense and Flavobacterium rivuli . Int J Syst Evol Microbiol 63: 3237–3242 [CrossRef] [PubMed].
    [Google Scholar]
  9. Felsenstein J. . ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  10. Felsenstein J. . ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  11. Feng Q. , Han L. , Yuan X. , Tan X. , Gao Y. , Lv J. . ( 2015;). Flavobacterium procerum sp. nov., isolated from freshwater. Int J Syst Evol Microbiol 65: 2702–2708 [CrossRef] [PubMed].
    [Google Scholar]
  12. Fitch W. M. . ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20: 406–416 [CrossRef].
    [Google Scholar]
  13. Fu Y. , Tang X. , Lai Q. , Zhang C. , Zhong H. , Li W. , Liu Y. , Chen L. , Sun F. , Shao Z. . ( 2011;). Flavobacterium beibuense sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 61: 205–209 [CrossRef] [PubMed].
    [Google Scholar]
  14. Glaeser S. P. , Galatis H. , Martin K. , Kämpfer P. . ( 2013;). Flavobacterium cutihirudinis sp. nov., isolated from the skin of the medical leech Hirudo verbana . Int J Syst Evol Microbiol 63: 2841–2847 [CrossRef] [PubMed].
    [Google Scholar]
  15. Humphry D. R. , George A. , Black G. W. , Cummings S. P. . ( 2001;). Flavobacterium frigidarium sp. nov., an aerobic, psychrophilic, xylanolytic and laminarinolytic bacterium from Antarctica. Int J Syst Evol Microbiol 51: 1235–1243 [CrossRef] [PubMed].
    [Google Scholar]
  16. Joung Y. , Kim H. , Joh K. . ( 2013;). Flavobacterium jumunjinense sp. nov., isolated from a lagoon, and emended descriptions of Flavobacterium cheniae, Flavobacterium dongtanense and Flavobacterium gelidilacus . Int J Syst Evol Microbiol 63: 3937–3943 [CrossRef] [PubMed].
    [Google Scholar]
  17. Kacagan M. , Inan K. , Belduz A. O. , Canakci S. . ( 2013;). Flavobacterium anatoliense sp. nov., isolated from fresh water, and emended description of Flavobacterium ceti . Int J Syst Evol Microbiol 63: 2075–2081 [CrossRef] [PubMed].
    [Google Scholar]
  18. Kaur I. , Kaur C. , Khan F. , Mayilraj S. . ( 2012;). Flavobacterium rakeshii sp. nov., isolated from marine sediment, and emended description of Flavobacterium beibuense Fu et al. 2011. Int J Syst Evol Microbiol 62: 2897–2902 [CrossRef] [PubMed].
    [Google Scholar]
  19. Kim J. H. , Kim K. Y. , Cha C. J. . ( 2009;). Flavobacterium chungangense sp. nov., isolated from a freshwater lake. Int J Syst Evol Microbiol 59: 1754–1758 [CrossRef] [PubMed].
    [Google Scholar]
  20. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  21. Kimura M. . ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111–120 [CrossRef] [PubMed].
    [Google Scholar]
  22. Krieg N. R. , Padgett P. J. . ( 2011;). Phenotypic and physiological characterization methods. Methods Microbiol 38: 15–60 [PubMed].[CrossRef]
    [Google Scholar]
  23. Kroppenstedt R. M. . ( 1982;). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5: 2359–2367 [CrossRef].
    [Google Scholar]
  24. Kuo I. , Saw J. , Kapan D. D. , Christensen S. , Kaneshiro K. Y. , Donachie S. P. . ( 2013;). Flavobacterium akiainvivens sp. nov., from decaying wood of Wikstroemia oahuensis, Hawai'i, and emended description of the genus Flavobacterium . Int J Syst Evol Microbiol 63: 3280–3286 [CrossRef] [PubMed].
    [Google Scholar]
  25. Lane D. J. . ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 125–175. Edited by Stackebrandt E. , Goodfellow M. . Chichester: Wiley;.
    [Google Scholar]
  26. Lee S. , Weon H. Y. , Kim S. J. , Ahn T. Y. . ( 2011;). Flavobacterium koreense sp. nov., Flavobacterium chungnamense sp. nov., and Flavobacterium cheonanense sp. nov., isolated from a freshwater reservoir. J Microbiol 49: 387–392 [CrossRef] [PubMed].
    [Google Scholar]
  27. Ludwig W. , Strunk O. , Westram R. , Richter L. , Meier H. , Yadhukumar , Buchner A. , Lai T. , Steppi S. , other authors . ( 2004;). arb: a software environment for sequence data. Nucleic Acids Res 32: 1363–1371 [CrossRef] [PubMed].
    [Google Scholar]
  28. McCammon S. A. , Innes B. H. , Bowman J. P. , Franzmann P. D. , Dobson S. J. , Holloway P. E. , Skerratt J. H. , Nichols P. D. , Rankin L. M. . ( 1998;). Flavobacterium hibernum sp. nov., a lactose-utilizing bacterium from a freshwater Antarctic lake. Int J Syst Bacteriol 48: 1405–1412 [CrossRef] [PubMed].
    [Google Scholar]
  29. Mesbah M. , Whitman W. B. . ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr A 479: 297–306 [CrossRef] [PubMed].
    [Google Scholar]
  30. Minnikin D. E. , O'Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinines and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  31. Nogi Y. , Yoshizumi M. , Miyazaki M. . ( 2014;). Thalassospira povalilytica sp. nov., a polyvinyl-alcohol-degrading marine bacterium. Int J Syst Evol Microbiol 64: 1149–1153 [CrossRef] [PubMed].
    [Google Scholar]
  32. Park M. , Lu S. , Ryu S. H. , Chung B. S. , Park W. , Kim C. J. , Jeon C. O. . ( 2006;). Flavobacterium croceum sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 56: 2443–2447 [CrossRef] [PubMed].
    [Google Scholar]
  33. Park M. , Ryu S. H. , Vu T. H. , Ro H. S. , Yun P. Y. , Jeon C. O. . ( 2007;). Flavobacterium defluvii sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 57: 233–237 [CrossRef] [PubMed].
    [Google Scholar]
  34. Qu J. H. , Yuan H. L. , Li H. F. , Deng C. P. . ( 2009;). Flavobacterium cauense sp. nov., isolated from sediment of a eutrophic lake. Int J Syst Evol Microbiol 59: 2666–2669 [CrossRef] [PubMed].
    [Google Scholar]
  35. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  36. Sheu S. Y. , Chiu T. F. , Young C. C. , Arun A. B. , Chen W. M. . ( 2011;). Flavobacterium macrobrachii sp. nov., isolated from a freshwater shrimp culture pond. Int J Syst Evol Microbiol 61: 1402–1407 [CrossRef] [PubMed].
    [Google Scholar]
  37. Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. . ( 2013;). MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  38. Yang F. , Liu H. M. , Zhang R. , Chen D. B. , Wang X. , Yan X. , Hong Q. , Li S. P. . ( 2015;). Flavobacterium shanxiense sp. nov., isolated from soil. Curr Microbiol 70: 835–839 [CrossRef] [PubMed].
    [Google Scholar]
  39. Yarza P. , Richter M. , Peplies J. , Euzeby J. , Amann R. , Schleifer K. H. , Ludwig W. , Glöckner F. O. , Rosselló-Móra R. . ( 2008;). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol 31: 241–250 [CrossRef] [PubMed].
    [Google Scholar]
  40. Yi H. , Oh H. M. , Lee J. H. , Kim S. J. , Chun J. . ( 2005;). Flavobacterium antarcticum sp. nov., a novel psychrotolerant bacterium isolated from the Antarctic. Int J Syst Evol Microbiol 55: 637–641 [CrossRef] [PubMed].
    [Google Scholar]
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