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Abstract

Gram-stain-negative, oxidase-negative, facultatively anaerobic, motile, rod-shaped, non-pigmented bacterial strains (N6PO6, N8PO1 and N8PI1) were isolated from the mirid bug captured on plants. The 16S rRNA gene sequences of the strains shared 94.7–95.7 % similarity with species of the genus and 95.6 % or less with species from other genera in the family . A polyphasic approach that included determination of phenotypic properties and phylogenetic analysis based on 16S rRNA, , and gene sequences supported the classification of strains N6PO6, N8PO1 and N8PI1 as representing a novel species of a new genus in the family . Strain N6PO6, and the two reference strains of the novel species, grew at 1–37 °C, and in the presence of NaCl (up to 7.5 %, w/v) and sucrose (up to 60 %). Their major cellular fatty acids were C, C cyclo, Cω7, summed feature 2 (C 3-OH and/or iso-C I) and summed feature 3 (Cω7 and/or iso-C 2-OH). The DNA G+C content of strain N6PO6 was 49.9 mol%. On the basis of phenotypic properties and phylogenetic distinctiveness, the mirid bug isolates are classified as representing a novel species in a new genus , in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is N6PO6 ( = LMG 28430 = DSM 29293).

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2016-03-01
2020-01-20
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