1887

Abstract

A novel heterotrophic, aerobic, Gram-stain-negative, rod-shaped and yellow bacterium, designated strain G18, was isolated from a water sample collected from the deep South China Sea. Strain G18 grew at 4–40 °C (optimum 28–32 °C), at pH 6.0–8.0 (optimum pH 6.5–7.5) and with 0–12 % (w/v) NaCl (optimum 3–4 %). The organism was mesophilic and piezotolerant, its optimal growth pressure was 0.1 MPa, which was lower than that at the depth from which it was isolated. Its optimal growth temperature was higher than that at the depth of its isolation. The predominant cellular fatty acids were Ciso, Ciso 3-OH and Ciso. The major polar lipids were composed of phosphatidylethanolamine, one unknown aminolipid and one unknown polar lipid. The major respiratory quinone was menaquinone 6. The G+C content of the genomic DNA was 35 mol%. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain G18 clustered with species of the genus with validly published names within the family with 95.9–98.2 % sequence similarity. DNA–DNA reassociation values ranged from 9 to 42 %. Differential phenotypic properties, together with the phylogenetic distinctiveness, suggest that strain G18 differs from species of the genus with validly published names. On the basis of the polyphasic evidence, strain G18 represents a novel species, isolated from deep-sea, of the genus for which the name sp. nov. is proposed. The type strain is G18 ( = CCTCC AB 2015204 = KCTC 42729).

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2016-03-01
2021-07-23
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