1887

Abstract

A Gram-stain-positive, aerobic, rod-shaped, non-motile actinomycete strain, designated EGI 60007, was isolated from healthy roots of F. collected from Yili County, Xinjiang Province, north-west China. A polyphasic approach was applied to study the taxonomic position of the new isolate. The 16S rRNA gene sequence of strain EGI 60007 had highest similarities with members of the genus , including CAU 59 (96.98 % 16S rRNA gene sequence similarity), DSM 20419 (96.43 %) and THG-MD12 (96.14 %). The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain EGI 60007 clustered with members of the genus , and formed a distinct clade with CAU 59. The polar lipids detected for strain EGI 60007 were phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and one unidentified lipid. The DNA G+C content was determined to be 63.3 mol%. The chemotaxonomic features of strain EGI 60007 showed typical characteristics of the genus , with MK-9 as the respiratory quinone, 2,4-diaminobutyric acid as the diamino acid in the peptidoglycan, and anteiso-C, anteiso-C and iso-C as the major fatty acids. The sugars of whole-cell hydrolysates were mainly mannose, rhamnose, ribose and glucose, and a minor amount of xylose. Based on the results of the phylogentic analysis supported by morphological, physiological, chemotaxonomic and other differentiating phenotypic characteristics, strain EGI 60007 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EGI 60007 ( = CGMCC 1.15081 = KCTC 39112 = DSM 29943).

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2016-03-01
2019-08-22
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References

  1. Cappuccino J. G., Sherman N.. ( 2002;). Microbiology: a Laboratory Manual, 6th edn.., Menlo Park, CA: Benjamin Cummings;.
    [Google Scholar]
  2. Cerny G.. ( 1978;). Studies on the aminopeptidase test for the distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5: 113–122 [CrossRef].
    [Google Scholar]
  3. Cho S. L., Jung M. Y., Park M. H., Chang Y. H., Yoon J. H., Myung S. C., Kim W.. ( 2010;). Pseudoclavibacter chungangensis sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 60: 1672–1677 [CrossRef] [PubMed].
    [Google Scholar]
  4. Collins M. D., Jones D.. ( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycan based on 2,4-diaminobutyric acid. J Appl Bacteriol 48: 459–470 [CrossRef].
    [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100: 221–230 [CrossRef] [PubMed].
    [Google Scholar]
  6. Du J., Singh H., Yang J.-E., Yin C. S., Kook M., Yu H., Yi T.-H.. ( 2015;). Pseudoclavibacter terrae sp. nov. isolated from rhizophere soil of Ophiopogon japonicas. Int J Syst Evol Microbiol 65: 4202–4207 [CrossRef] [PubMed].
    [Google Scholar]
  7. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  8. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  9. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20: 406–416 [CrossRef].
    [Google Scholar]
  10. Kim M. K., Jung H. Y.. ( 2009;). Pseudoclavibacter soli sp. nov., a β-glucosidase-producing bacterium. Int J Syst Evol Microbiol 59: 835–838 [CrossRef] [PubMed].
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  12. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111–120 [CrossRef] [PubMed].
    [Google Scholar]
  13. Kroppenstedt R. M.. ( 1982;). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5: 2359–2367 [CrossRef].
    [Google Scholar]
  14. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A., other authors. ( 2007;). clustal w clustal_x version 2.0. Bioinformatics 23: 2947–2948 [CrossRef] [PubMed].
    [Google Scholar]
  15. Lechevalier M. P., Lechevalier H. A.. ( 1970;). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20: 435–443 [CrossRef].
    [Google Scholar]
  16. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L.. ( 2007;). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57: 1424–1428 [CrossRef] [PubMed].
    [Google Scholar]
  17. Manaia C. M., Nogales B., Weiss N., Nunes O. C.. ( 2004;). Gulosibacter molinativorax gen. nov., sp. nov., a molinate-degrading bacterium, and classification of ‘Brevibacterium helvolum’ DSM 20419 as Pseudoclavibacter helvolus gen. nov., sp. nov. Int J Syst Evol Microbiol 54: 783–789 [CrossRef] [PubMed].
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  19. Minnikin D. E., Collins M. D., Goodfellow M.. ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47: 87–95 [CrossRef].
    [Google Scholar]
  20. Parte A. C.. ( 2014;). LPSN–list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42: (D1), D613–D616 [CrossRef] [PubMed].
    [Google Scholar]
  21. Qin S., Li J., Chen H. H., Zhao G. Z., Zhu W. Y., Jiang C. L., Xu L. H., Li W. J.. ( 2009;). Isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China. Appl Environ Microbiol 75: 6176–6186 [CrossRef] [PubMed].
    [Google Scholar]
  22. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  23. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  24. Schleifer K. H., Kandler O.. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36: 407–477 [PubMed].
    [Google Scholar]
  25. Srinivasan S., Kim H. S., Kim M. K., Lee M.. ( 2012;). Pseudoclavibacter caeni sp. nov., isolated from sludge of a sewage disposal plant. Int J Syst Evol Microbiol 62: 786–790 [CrossRef] [PubMed].
    [Google Scholar]
  26. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013;). MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  27. Tang S. K., Wang Y., Chen Y., Lou K., Cao L. L., Xu L. H., Li W. J.. ( 2009;). Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. Int J Syst Evol Microbiol 59: 2025–2031 [CrossRef] [PubMed].
    [Google Scholar]
  28. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L.. ( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55: 1149–1153 [CrossRef] [PubMed].
    [Google Scholar]
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