1887

Abstract

Twenty-three rod-shaped, endospore-forming, Gram-stain-positive, obligately anaerobic bacteria were isolated from different marine sediment samples of Gujarat. All 23 strains shared 16S rRNA gene sequence similarity of ∼100 %. Strain JC272 was designated the type strain and shared highest sequence similarity with ATCC 638 (99.8 %), JCM 1400 (98.0 %), ATCC 9714 (97.9 %) and other members of the genus ( < 96.4 %). C, C, C, Cω9 and iso-C were the major (>5 %) fatty acids. Strain JC272 contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and two unidentified amino lipids. Genome-based analysis of average nucleotide identity (ANI) and DNA–DNA hybridization (DDH) of strain JC272 with ATCC 638 yielded values of 94.35 and 58.5 ± 2.8 %, respectively. The DNA G+C content of strain JC272 was 28.3 mol%. Strain JC272 together with were found to fall outside rRNA cluster I considered as . Based on ANI value, DDH, and distinct morphological and physiological differences from the previously described taxa, we suggest that strain JC272 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is JC272 ( = KCTC 15476 = LMG 28745). It is also proposed to transfer to this new genus, as comb. nov. (type strain ATCC 638 = DSM 14991 = JCM 1386). The genus gen. nov. is proposed to accommodate and as comb. nov. (type strain ATCC 9714 = LMG 15708 = JCM 3814) and comb. nov. (type strain ATCC 25757 = DSM 15049 = JCM 1400).

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2016-03-01
2019-12-06
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