1887

Abstract

A Gram-stain-negative, obligately aerobic, non-motile, non-sporulating, rod-shaped bacterium, designated TZCO2, was isolated from the soil of an irrigated coffee plantation in Arusha, Tanzania, East Africa. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that the isolate is affiliated with the genus in the family . Its closest relative is THG-DT86 (96.7 %). The pH and temperature ranges for growth were pH 6.0–8.5 (optimum 7.0–7.5) and 10–35 °C (optimum 30 °C, respectively. The predominant fatty acids were iso-C (32.4 %), iso-C G (22.6 %), iso-C (15.1 %) and iso-C 3-OH (10.0 %) The only isoprenoid quinone detected in strain TZCO2 was menaquinone-7 (MK-7); the major polar lipids were phosphoaminolipid, phosphatidylethanolamine, unidentified aminolipids and lipids. The DNA G+C content was 51.9 mol%. Physiological and chemotaxonomic data further confirmed that strain TZCO2 is distinct from other members of the genus . Thus, strain TZCO2 is considered to represent a novel species of the genus, for which the name sp. nov. is proposed. The type strain is TZCO2 ( = NCAIM B 02601 = CCM 8601).

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2016-04-01
2020-01-24
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