%0 Journal Article %A Joshi, Amaraja %A Thite, Sonia %A Kulkarni, Girish %A Dhotre, Dhiraj %A Joseph, Neetha %A Venkata Ramana, V. %A Polkade, Ashish %A Shouche, Yogesh %T Nitrincola alkalisediminis sp. nov., an alkaliphilic bacterium isolated from an alkaline lake %D 2016 %J International Journal of Systematic and Evolutionary Microbiology, %V 66 %N 3 %P 1254-1259 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.000868 %I Microbiology Society, %X Two Gram-stain-negative, aerobic, alkaliphilic bacteria (strains MEB087T and MEB142) were isolated from sediment and water samples, respectively, collected from the alkaline Lonar Lake in Maharashtra, India. Strains MEB087T and MEB142 shared 99.8 % 16S rRNA gene sequence similarity and were 85 % related on the basis of DNA–DNA hybridization. The 16S rRNA gene sequences of both strains showed close relationship with the genus Nitrincola, and their closest neighbour was Nitrincola lacisaponensis 4CAT with 97.7 % sequence similarity. MEB087T and MEB142 exhibited only 45 % and 54 % DNA–DNA relatedness, respectively, with Nitrincola lacisaponensis DSM 16316T. Both strains were asporogenous, short, non-motile rods capable of utilizing a limited range of organic acids as sole carbon and energy sources. They were oxidase- and catalase-positive, able to reduce nitrate and nitrite; but unable to degrade DNA, urea, gelatin, casein or starch. They grew optimally at pH 9.5 (tolerating up to pH 11) and could withstand up to 0.6 M NaCl. The predominant cellular fatty acids were summed feature 8 comprising C18 : 1ω7c/C18 : 1ω6c (47–49 %) followed by summed feature 3 comprising C16 : 1ω7c/C16 : 1ω6c (28–32 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The DNA G+C content was 49.3–49.7 mol%. On the basis of the phylogenetic analysis and chemotaxonomic characteristics, strains MEB087T and MEB142 represent a novel species in the genus Nitrincola, for which the name Nitrincola alkalisediminis sp. nov. is proposed. The type strain is MEB087T ( = KCTC 42948T = JCM 19317T) with MEB142 ( = KCTC 42949 = JCM 19318) as an additional strain. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000868