1887

Abstract

A novel acidotolerant and moderately thermophilic sulfur-reducing bacterium was isolated from sediments of the Tinto River (Spain), an extremely acidic environment. Strain TR1 stained Gram-negative, and was obligately anaerobic, non-spore-forming and motile. Cells were short rods (1.5–2 × 0.5–0.7 μm), appearing singly or in pairs. Strain TR1 was catalase-negative and slightly oxidase-positive. Urease activity and indole formation were absent, but gelatin hydrolysis was present. Growth was observed at 20–52 °C with an optimum close to 50 °C, and a pH range of 3–7 with optimum between pH 6 and 6.5. Yeast extract was essential for growth, but extra vitamins were not required. In the presence of sulfur, strain TR1 grew with acetate, formate, lactate, pyruvate, stearate, arginine and H/CO. All substrates were completely oxidized and HS and CO were the only metabolic products detected. Besides elemental sulfur, thiosulfate was used as an electron acceptor. The isolate also grew by disproportionation of elemental sulfur. The predominant cellular fatty acids were saturated components: C, anteiso-C and C. The only quinone component detected was menaquinone MK-7(H). The G+C content of the genomic DNA was 34 mol%. The isolate is affiliated to the genus of the class , sharing 97 % 16S rRNA gene sequence similarity with the four species described in the genus . Considering the distinct physiological and phylogenetic characteristics, strain TR1 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is TR1 ( = DSM 29984 = JCM 30680).

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/content/journal/ijsem/10.1099/ijsem.0.000866
2016-03-01
2024-03-28
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References

  1. Bonch-Osmolovskaya E. A., Sokolova T. G., Kostrikina N. A., Zavarzin G. A. 1990; Desulfurella acetivorans gen. nov. and sp. nov. – a new thermophilic sulfur-reducing eubacterium. Arch Microbiol 153:151–155 [View Article]
    [Google Scholar]
  2. Brito E. M., Villegas-Negrete N., Sotelo-Gonzalez I. A., Caretta C. A., Goñi-Urriza M., Gassie C., Hakil F., Colin Y., Duran R., other authors. 2014; Microbial diversity in Los Azufres geothermal field (Michoacan, Mexico) and isolation of representative sulfate and sulfur reducers. Extremophiles 18:385–398 [CrossRef]
    [Google Scholar]
  3. Cline J. D. 1969; Spectrophotometric determination of hydrogen sulfide in natural waters. Limnol Oceanogr 14:454–458 [View Article]
    [Google Scholar]
  4. Doetsch R. N. 1981; Determinative methods of light microscopy. In Manual of Methods for General Bacteriology pp 21–33Edited by Gerdhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Florentino A. P., Weijma J., Stams A. J. M., Sánchez-Andrea I. 2015; Sulfur reduction in acid rock drainage environments. Environ Sci Technol 49:11746–11755 [CrossRef]
    [Google Scholar]
  6. Florentino A. P., Weijma J., Stams A. J. M., Sánchez-Andrea I. 2016; Ecophysiology and application of acidophilic sulfur-reducing microorganisms. In Biotechnology of Extremophiles: Advances and ChallengesEdited by Rampelotto P. H. Cham: Springer International Publishing, in press;
    [Google Scholar]
  7. Koschorreck M. 2008; Microbial sulphate reduction at a low pH. FEMS Microbiol Ecol 64:329–342 [View Article][PubMed]
    [Google Scholar]
  8. Miroshnichenko M. L., Gongadze G. A., Lysenko A. M., Bonch-Osmolovskaya E. A. 1994; Desulfurella multipotens sp. nov., a new sulfur-respiring thermophilic eubacterium from Raoul Island (Kermadec archipelago, New Zealand). Arch Microbiol 161:88–93
    [Google Scholar]
  9. Miroshnichenko M. L., Rainey F. A., Hippe H., Chernyh N. A., Kostrikina N. A., Bonch-Osmolovskaya E. A. 1998; Desulfurella kamchatkensis sp. nov. and Desulfurella propionica sp. nov., new sulfur-respiring thermophilic bacteria from Kamchatka thermal environments. Int J Syst Bacteriol 48:475–479 [View Article][PubMed]
    [Google Scholar]
  10. Miroshnichenko M. L., Rainey F. A., Rhode M., Bonch-Osmolovskaya E. A. 1999; Hippea maritima gen. nov., sp. nov., a new genus of thermophilic, sulfur-reducing bacterium from submarine hot vents. Int J Syst Bacteriol 49:1033–1038 [View Article][PubMed]
    [Google Scholar]
  11. Rabus R., Hansen T. A., Widdel F. 2006; Dissimilatory sulfate- and sulfur-reducing prokaryotes. In The Prokaryotes pp 659–768Edited by Dworkin M., Schleifer K.-H., Stackebrandt E. New York: Springer; [View Article]
    [Google Scholar]
  12. Sánchez-Andrea I., Rodríguez N., Amils R., Sanz J. L. 2011; Microbial diversity in anaerobic sediments at Rio Tinto, a naturally acidic environment with a high heavy metal content. Appl Environ Microbiol 77:6085–6093 [View Article][PubMed]
    [Google Scholar]
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