@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.000865, author = "Sravanthi, T. and Tushar, L. and Sasikala, Ch. and Ramana, Ch. V.", title = "Alkalispirochaeta cellulosivorans gen. nov., sp. nov., a cellulose-hydrolysing, alkaliphilic, halotolerant bacterium isolated from the gut of a wood-eating cockroach (Cryptocercus punctulatus), and reclassification of four species of Spirochaeta as new combinations within Alkalispirochaeta gen. nov.", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "4", pages = "1612-1619", doi = "https://doi.org/10.1099/ijsem.0.000865", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000865", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "An obligately anaerobic spirochaete designated strain JC227T was isolated from the gut of a wood-eating cockroach, Cryptocercus punctulatus (Scudder), from the Rann of Kutch, Gujarat, India. Strain JC227T was Gram-stain-negative, mesophilic, halotolerant and alkaliphilic. Based on 16S rRNA gene sequence analysis, strain JC227T belongs to the genus Spirochaeta, with Spirochaeta sphaeroplastigenens JC133T (99.51 %), S. odontotermitis JC202T (99.30 %), S. alkalica Z-7491T (99.10 %), S. americana (98.54 %) and other members of the genus Spirochaeta ( < 92.7 %) as its closest phylogenetic neighbours. However, DNA–DNA hybridization between strain JC227T and S. sphaeroplastigenens JC133T, S. odontotermitis JC202T, S. alkalica DSM 8900T and S. americana DSM 14872T was 62 ± 2, 63, 58 ± 2 and 48 ± 4 %, respectively. Strain JC227T contained phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid and six unidentified lipids. Summed feature C18 : 1ω7c/C18 : 1ω6c was the predominant cellular fatty acid, with significant proportions of C16 : 0, C14 : 0, C12 : 0, C15 : 1ω6c, C16 : 1ω5c, C16 : 1ω6c/C16 : 1ω7c and C17 : 0 2-OH. The DNA G+C content of strain JC227T was 55.5 mol%. On the basis of physiological, biochemical, chemotaxonomic (including metabolomic) and genomic differences from previously described taxa, strain JC227T can be differentiated from members of the genus Spirochaeta and represents a novel species of a new genus, for which the name Alkalispirochaeta cellulosivorans gen. nov., sp. nov. is proposed. The type strain of Alkalispirochaeta cellulosivorans is JC227T ( = KCTC 15343T = NBRC 110105T). We also propose the reclassification of Spirochaeta sphaeroplastigenens, Spirochaeta odontotermitis, Spirochaeta alkalica and Spirochaeta americana as Alkalispirochaeta sphaeroplastigenens comb. nov. (type strain JC133T = KCTC 15220T = NBRC 109056T), Alkalispirochaeta odontotermitis comb. nov. (type strain JC202T = KCTC 15324T = NBRC 110104T), Alkalispirochaeta alkalica comb. nov. (type strain Z-7491T = DSM 8900T = ATCC 700262T) and Alkalispirochaeta americana comb. nov. (type strain ASpG1T = ATCC BAA-392T = DSM 14872T). The type species of Alkalispirochaeta gen. nov. is Alkalispirochaeta alkalica comb. nov.", }