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Volume 66, Issue 3

sp. nov., isolated from soil Free

Abstract

A novel Gram-stain-positive strain with sandy aerial mycelium and golden yellow substrate mycelium, designated fd2-tb, was isolated from a soil sample collected in Shanghai, China, and its taxonomic status was established by phylogenetic analysis. 16S rRNA gene sequence analysis showed that strain fd2-tb belonged to the genus and was related to JCM 19660 (99.9 % 16S rRNA gene sequence similarity), NBRC 12770 (99.9 %), NBRC 13072 (99.8 %), NRRL B-5430 (99.7 %), LMG 19896 (99.5 %), NBRC 12823 (99.5 %) and NBRC 13447 (99.3 %). The cell wall of strain fd2-tb contained -diaminopimelic acid, and whole-cell sugars were identified as glucose and ribose. The menaquinones MK-9(H), MK-9(H) and MK-9(H) were also detected. In addition, the polar lipids diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, as well as five unidentified phospholipids, were detected. Major cellular fatty acids were identified as anteiso-C, iso-C, anteiso-C, iso-C and C. DNA–DNA hybridization experiments showed that strain fd2-tb exhibited 36.5 ± 0.6 %, 43.5 ± 2.0 %, 11.1 ± 1.3 %, 10.3 ± 3.1 %, 9.8 ± 1.9 %, 48.9 ± 3.9 % and 16.3 ± 1.7 % relatedness to JCM 40119660, NBRC 12770, NBRC 13072, NRRL B-5430, LMG 19896, NBRC 12823 and NBRC 13447, respectively. Based on these analyses as well as some phenotypic differences, strain fd2-tb is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is fd2-tb ( = DSM 100336 = CGMCC 4.7256).

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2016-03-01
2024-04-20
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Streptomyces yangpuensis sp. nov., isolated from soil
Int J Syst Evol Microbiol 66, 1224 (2016); https://doi.org/10.1099/ijsem.0.000861
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