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Abstract

An aerobic, Gram-stain-positive, motile, coccus-shaped actinobacterium, designated strain L2-1-L1, was isolated from mangrove sediment in Thailand. The organism was deep orange, oxidase-negative and catalase-positive. Growth occurred at temperatures between 17 and 32 °C and with NaCl concentrations up to 10 %. Chemotaxonomic characteristics of strain L2-1-L1 were typical of the genus . The diagnostic diamino acid of the peptidoglycan was -diaminopimelic acid. Whole-cell hydrolysates contained arabinose, galactose, glucose, mannose and ribose. The menaquinone was MK-9(H). Mycolic acids were not detected. Anteiso-C and iso-C were detected as the major cellular fatty acids. The major polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol and an unidentified phosphoglycolipid. The G+C content of the DNA was 74.7 mol%. A phylogenetic tree, based on 16S rRNA gene sequences, revealed that strain L2-1-L1 represents a novel member of the genus . The most closely related species were KLBMP 1274, NBRC 15268 and RP-B16, with 98.9, 98.6 and 98.5 % 16S rRNA gene sequence similarity, respectively. On the basis of phylogenetic analysis, DNA–DNA relatedness data, phenotypic characteristics and chemotaxonomic data, a novel species of the genus is proposed: sp. nov. The type strain is strain L2-1-L1 ( = BCC 75409 = NBRC 110933).

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2016-03-01
2022-01-21
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