A novel actinobacterial strain, YIM ART06T, was isolated from a rock sample of karst cave located at Guizhou province, south-west China, and was characterized by a polyphasic taxonomic approach. The morphological and chemotaxonomic properties of strain YIM ART06T were in accordance with those of the genus Stackebrandtia. The 16S rRNA gene sequence of strain YIM ART06T showed highest similarity to Stackebrandtia nassauensis JCM 14905T (98.0 %). The DNA–DNA hybridization value between strains YIM ART06T and S. nassauensis JCM 14905T was, however, moderately high (62.9 %) but below the 70 % limit for species identification. Strain YIM ART06T contained meso-diaminopimelic acid as the diagnostic diamino acid, and mannose, ribose and xylose in the whole-cell hydrolysates. The predominant menaquinones detected were MK-10(H4), MK-10(H6), MK-11(H4) and MK-11(H6), while the cell membrane polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmethylethanolamine and three unidentified phospholipids. The genomic DNA G+C content of strain YIM ART06T was 71 mol%. The major fatty acids were anteiso-C17 : 0, iso-C17 : 0, iso-C16 : 0 and iso-C15 : 0. Based on the taxonomic characteristics from the genotypic and phenotypic results, strain YIM ART06T merits recognition as a representative of a novel species of the genus Stackebrandtia, for which the name Stackebrandtia cavernae sp. nov. is proposed. The type strain is YIM ART06T ( = KCTC 39599T = CCTCC AA 2015021T = DSM 100594T).
CollinsM. D.,
JonesD.1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycan based on 2, 4-diaminobutyric acid. J Appl Bacteriol 48:459–470 [View Article]
CollinsM. D.,
PirouzT.,
GoodfellowM.,
MinnikinD. E.1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [View Article][PubMed]
CuiX.-L.,
MaoP.-H.,
ZengM.,
LiW.-J.,
ZhangL.-P.,
XuL.-H.,
JiangC.-L.2001; Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae
. Int J Syst Evol Microbiol 51:357–363 [View Article][PubMed]
EmbleyT. M.,
WaitR.1994; Structural lipids of eubacteria. In Chemical Methods in Prokaryotic Systematics pp 121–161Edited byGoodfellowM.,
O'DonnellA. G.
Chichester: Wiley;
EzakiT.,
HashimotoY.,
YabuuchiE.1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
GuanT.-W.,
TangS.-K.,
WuJ.-Y.,
ZhiX.-Y.,
XuL.-H.,
ZhangL.-L.,
LiW.-J.2009; Haloglycomyces albus gen. nov., sp. nov., a halophilic, filamentous actinomycete of the family Glycomycetaceae
. Int J Syst Evol Microbiol 59:1297–1301 [View Article][PubMed]
KellyK. L.1964Inter-Society Color Council – National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
KimuraM.1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
KroppenstedtR. M.1982; Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5:2359–2367 [View Article]
LabedaD. P.,
KroppenstedtR. M.2004; Emended description of the genus Glycomyces and description of Glycomyces algeriensis sp. nov., Glycomyces arizonensis sp. nov. and Glycomyces lechevalierae sp. nov. Int J Syst Evol Microbiol 54:2343–2346 [View Article][PubMed]
LabedaD. P.,
KroppenstedtR. M.2005; Stackebrandtia nassauensis gen. nov., sp. nov. and emended description of the family Glycomycetaceae
. Int J Syst Evol Microbiol 55:1687–1691 [View Article][PubMed]
LabedaD. P.,
TestaR. T.,
LechevalierM. P.,
LechevalierH. A.1985; Glycomyces, a new genus of the Actinomycetales
. Int J Syst Bacteriol 35:417–421 [View Article]
LiW.-J.,
XuP.,
SchumannP.,
ZhangY.-Q.,
PukallR.,
XuL.-H.,
StackebrandtE.,
JiangC. L.2007; Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia
. Int J Syst Evol Microbiol 57:1424–1428 [View Article][PubMed]
LocciR.1989; Streptomyces and related genera. In Bergey's Manual of Systematic Bacteriologyvol. 4 pp 2451–2508Edited byWilliamsS. T.,
SharpeM. E.,
HoltJ. G.
Baltimore: Williams & Wilkins;
MesbahM.,
PremachandranU.,
WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
MinnikinD. E.,
CollinsM. D.,
GoodfellowM.1979; Fatty acid and polar lipid composition in the classification of Cellulomonas,
Oerskovia and related taxa. J Appl Bacteriol 47:87–95 [View Article]
ReasonerD. J.,
GeldreichE. E.1985; A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49:1–7[PubMed]
StackebrandtE.,
GoebelB. M.1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
StackebrandtE.,
RaineyF. A.,
Ward-RaineyN. L.1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [View Article]
TangS.-K.,
WangY.,
ChenY.,
LouK.,
CaoL.-L.,
XuL.-H.,
LiW.-J.2009; Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella
. Int J Syst Evol Microbiol 59:2025–2032 [View Article][PubMed]
ThomasT. D.,
JarvisB. D. W.,
SkipperN. A.1974; Localization of proteinase(s) near the cell surface of Streptococcus lactis
. J Bacteriol 118:329–333[PubMed]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
WilliamsS. T.,
GoodfellowM.,
AldersonG.1989; Genus Streptomyces Waksman and Henrici 1943, 339AL
. In Bergey's Manual of Systematic Bacteriologyvol. 4 pp 2453–2492Edited byWilliamsS. T.,
SharpeM. E.,
HoltJ. G.
Baltimore: Williams & Wilkins;
XuP.,
LiW.-J.,
TangS.-K.,
ZhangY.-Q.,
ChenG.-Z.,
ChenH.-H.,
XuL.-H.,
JiangC.-L.2005; Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153 [View Article][PubMed]