1887

Abstract

was previously reported to be a later heterotypic synonym of , based primarily on DNA–DNA relatedness values. We have sequenced a draft genome of NRRL B-41580. Comparative genomics and DNA–DNA relatedness calculations show that it is not a synonym of It was instead synonymous with ’ is a recently described species that was isolated as an endophyte of rice (). The strain was demonstrated to have plant-pathogen antagonist activity in greenhouse assays, and the 16S rRNA gene was reported to have 99.7 % sequence similarity with and , which are both known for their plant pathogen antagonism. To better understand the phylogenetics of these closely related strains, we sequenced the genome of ‘. ’ KACC 18228. Comparative genomic analysis showed only minor differences between this strain and the genomes of NRRL B-41580, KACC 13015 and subsp. FZB42. The pairwise DNA–DNA hybridization values calculated in comparisons between the strains were all greater than 84 %, which is well above the standard species threshold of 70 %. The results of morphological, physiological, chemotaxonomic and phylogenetic analyses indicate that the strains share phenotype and genotype coherence. Therefore, we propose that KACC 13015, subsp. FZB42, and ‘ KACC 18228 should be reclassified as later heterotypic synonyms of NRRL B-41580, since the valid publication date of precedes the other three strains.

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2016-03-01
2019-09-21
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