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Abstract

A novel psychrotolerant bacterium, designed strain M6-79, was isolated from an arctic glacial foreland soil sample collected from Ny-Ålesund in the Svalbard Archipelago, Norway. Cells of strain M6-79 were Gram-stain-negative, rod-shaped and produced a red-pigment. Strain M6-79 was strictly aerobic, non-motile, non-endospore-forming, oxidase-negative and catalase-positive. Based on 16S rRNA gene sequence analysis, strain M6-79 was phylogenetically related to TR53 (95.2 % 16S rRNA gene sequence similarity), TH-G33 (94.3 %), ‘ FW-3 (94.3 %), DS-48 (94.1 %) and 5N26 (94.1 %). The unique isoprenoid quinone detected in strain M6-79 was Q-9. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one unknown aminolipid and one unknown lipid. Strain M6-79 possessed Cω7, C and summed feature 3 (Cω6 and/or Cω7) as the predominant fatty acids, and the DNA G+C content was 71.2 mol%. Combined data from phylogenetic, phenotypic and chemotaxonomic studies revealed that strain M6-79 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain M6-79 ( = CCTCC AB 2013101 = LMG 28251).

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2016-03-01
2019-12-11
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References

  1. Bauer A. W., Kirby W. M. M., Sherris J. C., Turck M.. ( 1966;). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45: 493–496 [PubMed].
    [Google Scholar]
  2. Bibashi E., Sofianou D., Kontopoulou K., Mitsopoulos E., Kokolina E.. ( 2000;). Peritonitis due to Roseomonas fauriae in a patient undergoing continuous ambulatory peritoneal dialysis. J Clin Microbiol 38: 456–457 [PubMed].
    [Google Scholar]
  3. Busse H.-J., Auling G.. ( 1988;). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 11: 1–8 [CrossRef].
    [Google Scholar]
  4. Busse H.-J., Bunka S., Hensel A., Lubitz W.. ( 1997;). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 47: 698–708 [CrossRef].
    [Google Scholar]
  5. Doetsch R. N.. ( 1981;). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology, pp. 21–33. Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. H.. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  6. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  7. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  8. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20: 406–416 [CrossRef].
    [Google Scholar]
  9. Gallego V., Sánchez-Porro C., García M. T., Ventosa A.. ( 2006;). Roseomonas aquatica sp. nov., isolated from drinking water. Int J Syst Evol Microbiol 56: 2291–2295 [CrossRef] [PubMed].
    [Google Scholar]
  10. He D., Kim J. K., Jiang X. Y., Park H. Y., Sun C., Yu H. S., Yoon M. H., Kim S. C., Jin F. X., Im W. T.. ( 2014;). Roseomonas sediminicola sp. nov., isolated from fresh water. Antonie van Leeuwenhoek 105: 191–197 [CrossRef] [PubMed].
    [Google Scholar]
  11. Jiang C. Y., Dai X., Wang B. J., Zhou Y. G., Liu S. J.. ( 2006;). Roseomonas lacus sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 56: 25–28 [CrossRef] [PubMed].
    [Google Scholar]
  12. Kim D. U., Ka J. O.. ( 2014;). Roseomonas soli sp. nov., isolated from an agricultural soil cultivated with Chinese cabbage (Brassica campestris). Int J Syst Evol Microbiol 64: 1024–1029 [CrossRef] [PubMed].
    [Google Scholar]
  13. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  14. Kim S. J., Weon H. Y., Ahn J. H., Hong S. B., Seok S. J., Whang K. S., Kwon S. W.. ( 2013;). Roseomonas aerophila sp. nov., isolated from air. Int J Syst Evol Microbiol 63: 2334–2337 [CrossRef] [PubMed].
    [Google Scholar]
  15. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111–120 [CrossRef] [PubMed].
    [Google Scholar]
  16. Lopes A., Espirito Santo C., Grass G., Chung A. P., Morais P. V.. ( 2011;). Roseomonas pecuniae sp. nov., isolated from the surface of a copper-alloy coin. Int J Syst Evol Microbiol 61: 610–615 [CrossRef] [PubMed].
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  18. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E.. ( 1996;). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46: 1088–1092 [CrossRef] [PubMed].
    [Google Scholar]
  19. Rihs J. D., Brenner D. J., Weaver R. E., Steigerwalt A. G., Hollis D. G., Yu V. L.. ( 1993;). Roseomonas, a new genus associated with bacteremia and other human infections. J Clin Microbiol 31: 3275–3283 [PubMed].
    [Google Scholar]
  20. Rihs J. D., Brenner D. J., Weaver R. E., Steigerwalt A. G., Hollis D. G., Yu V. L.. ( 1993;). Roseomonas gen. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List 65. Int J Syst Bacteriol 48: 627.
    [Google Scholar]
  21. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  22. Sánchez-Porro C., Gallego V., Busse H. J., Kämpfer P., Ventosa A.. ( 2009;). Transfer of Teichococcus ludipueritiae and Muricoccus roseus to the genus Roseomonas, as Roseomonas ludipueritiae comb. nov. and Roseomonas rosea comb. nov., respectively, and emended description of the genus Roseomonas. Int J Syst Evol Microbiol 59: 1193–1198 [CrossRef] [PubMed].
    [Google Scholar]
  23. Sandoe J. A., Malnick H., Loudon K. W.. ( 1997;). A case of peritonitis caused by Roseomonas gilardii in a patient undergoing continuous ambulatory peritoneal dialysis. J Clin Microbiol 35: 2150–2152 [PubMed].
    [Google Scholar]
  24. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  25. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Woods W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  26. Subudhi C. P., Adedeji A., Kaufmann M. E., Lucas G. S., Kerr J. R.. ( 2001;). Fatal Roseomonas gilardii bacteremia in a patient with refractory blast crisis of chronic myeloid leukemia. Clin Microbiol Infect 7: 573–575 [CrossRef] [PubMed].
    [Google Scholar]
  27. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  28. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673–4680 [CrossRef] [PubMed].
    [Google Scholar]
  29. Tindall B. J.. ( 1990;). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66: 199–202 [CrossRef].
    [Google Scholar]
  30. Venkata Ramana V., Sasikala Ch., Takaichi S., Ramana Ch. V.. ( 2010;). Roseomonas aestuarii sp. nov., a bacteriochlorophyll-a containing alphaproteobacterium isolated from an estuarine habitat of India. Syst Appl Microbiol 33: 198–203 [CrossRef] [PubMed].
    [Google Scholar]
  31. Xie C. H., Yokota A.. ( 2003;). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49: 345–349 [CrossRef] [PubMed].
    [Google Scholar]
  32. Yoo S. H., Weon H. Y., Noh H. J., Hong S. B., Lee C. M., Kim B. Y., Kwon S. W., Go S. J.. ( 2008;). Roseomonas aerilata sp. nov., isolated from an air sample. Int J Syst Evol Microbiol 58: 1482–1485 [CrossRef] [PubMed].
    [Google Scholar]
  33. Yoon J. H., Kang S. J., Oh H. W., Oh T. K.. ( 2007;). Roseomonas terrae sp. nov. Int J Syst Evol Microbiol 57: 2485–2488 [CrossRef] [PubMed].
    [Google Scholar]
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