A Gram-reaction-positive, spore-forming, rod-shaped bacterium, designated NR1-3-2T, was isolated from fish sauce collected from a factory in Chonburi province, Thailand. Strain NR1-3-2T grew at pH 5–10 (optimum 7.5), at 21–48 °C (optimum 37 °C) and with 0–15 % (w/v) NaCl (optimum 1–3 %). The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. Menaquinone with seven isoprene units (MK-7) was the major isoprenoid quinone. The strain contained anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 as major cellular fatty acids. Diphosphatidylglycerol, phosphatidyl glycerol and one unknown glycolipid were detected as major polar lipids. On the basis of 16S rRNA gene sequence analysis, strain NR1-3-2T belonged to the genus Bacillus and was closely related to Bacillus iranensis DSM 23995T (97.4 % similarity). Strain NR1-3-2T exhibited low DNA–DNA relatedness (31.2–39.8 %) with B. iranensis DSM 23995T. The DNA G+C content was 44.2 mol%. On the basis of phenotypic characteristics, DNA–DNA relatedness and phylogenetic analyses, the strain is considered to represent a novel species of the genus Bacillus and the name Bacillus piscicola sp. nov. is proposed. The type strain is NR1-3-2T ( = JCM 19598T = LMG 28281T = PCU 340T = TISTR 2295T).
AcarJ. F.,
GoldsteinF. W.1991; Disk susceptibility testing. In Antibiotics in laboratory medicine, pp. 17-52. Edited by. LorianV.
, 3rd edn. Baltimore: Williams & Wilkins;
BarrowG. I.,
FelthamR. K. A.editors1993Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: [View Article] Cambridge University Press;
CarrascoI. J.,
MárquezM. C.,
XueY.,
MaY.,
CowanD. A.,
JonesB. E.,
GrantW. D.,
VentosaA.2007; Bacillus chagannorensis sp. nov., a moderate halophile from a soda lake in Inner Mongolia, China. Int J Syst Evol Microbiol 57:2084–2088 [View Article][PubMed]
EzakiT.,
HashimotoY.,
YabuuchiE.1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
KämpferP.,
KroppenstedtR. M.1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa.. Can J Microbiol 42:989–1005[CrossRef]
KanedaT.1968; Fatty acids in the genus Bacillus. II. Similarity in the fatty acid compositions of Bacillus thuringiensis,
Bacillus anthracis, and Bacillus cereus
. J Bacteriol 95:2210–2216[PubMed]
LimJ. M.,
JeonC. O.,
LeeS. M.,
LeeJ. C.,
XuL. H.,
JiangC. L.,
KimC. J.2006; Bacillus salarius sp. nov., a halophilic, spore-forming bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 56:373–377 [View Article][PubMed]
MinnikinD. E.,
O'DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
NamwongS.,
TanasupawatS.,
SmitinontT.,
VisessanguanW.,
KudoT.,
ItohT.2005; Isolation of Lentibacillus salicampi strains and Lentibacillus juripiscarius sp. nov. from fish sauce in Thailand. Int J Syst Evol Microbiol 55:315–320 [View Article][PubMed]
ParteA. C.2015List of prokaryotic names with standing in nomenclature: a folder available on the Internet [Last full update 12 October 2015]. http://www.bacterio.net
RoohiA.,
AhmedI.,
PaekJ.,
SinY.,
AbbasS.,
JamilM.,
ChangY. H.2014; Bacillus pakistanensis sp. nov., a halotolerant bacterium isolated from salt mines of the Karak Area in Pakistan. Antonie van Leeuwenhoek 105:1163–1172 [View Article][PubMed]
SlepeckyR. A.,
HemphillH. E.2006; The genus Bacillus - nonmedical. In The Prokaryotes: The Handbook on the Biology of Bacteria, 3rd edn. pp 530–562Edited byDworkinM.,
FalkowS.,
RosenbergE.,
SchleiferK. H.,
StackebrandtE.
Singapore: Springer Science Business media, LLC; [View Article]
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
TanasupawatS.,
VisessanguanW.2014; Fish fermentation. In Seafood Processing: Technology, Quality and Safety pp 177–207Edited byBoziarisI. S.
New York: Wiley;
TanasupawatS.,
PakdeetoA.,
NamwongS.,
ThawaiC.,
KudoT.,
ItohT.2006; Lentibacillus halophilus sp. nov from fish sauce in Thailand. Int J Syst Evol Microbiol 56:1859–1863 [View Article][PubMed]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
ThornleyM. J.1960; The differentiation of Pseudomonas from other Gram-negative bacteria on the basis of arginine metabolism. J Appl Bacteriol 23:37–52 [View Article]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.,
other authors.
1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
YoonJ. H.,
KimI. G.,
KangK. H.,
OhT. K.,
ParkY. H.2003; Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 53:1297–1303 [View Article][PubMed]