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Abstract

A Gram-stain-negative, non-flagellated, non-spore-forming bacterial strain motile by gliding, designated RSS1-6, was isolated from a golden sea squirt and its taxonomic position was investigated by using a polyphasic approach. Strain RSS1-6 grew optimally at 30–37 °C and in the presence of 1.0–4.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain RSS1-6 fell within the clade comprising species of the genus , clustering with the type strains of , and with which it exhibited 16S rRNA gene sequence similarity values of 98.5–99.5 %. Strain RSS1-6 contained MK-6 as the predominant menaquinone and iso-C, iso-C 3-OH and summed feature 3 (Cω7 and/or Cω6) as the major fatty acids. The major polar lipids of strain RSS1-6 were phosphatidylethanolamine, two unidentified lipids, one unidentified aminophospholipid and one unidentified glycolipid. The DNA G+C content was 32.5 mol% and the mean DNA–DNA relatedness values with the type strains of , and were 17.3–25.2 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain RSS1-6 is separated from other recognized species of the genus . On the basis of the data presented, strain RSS1-6 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RSS1-6 ( = KCTC 42702 = NBRC 111225).

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2016-03-01
2021-10-16
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