1887

Abstract

A Gram-stain-negative, rod-shaped, non-spore-forming, oxidase and catalase-positive, strictly aerobic bacterium, designated strain Gsoil 524, was isolated from the soil of a ginseng field and subjected to polyphasic taxonomic analysis. Phylogenetic analysis, based on the 16S rRNA gene sequence, placed Gsoil 524 in a distinct lineage in the family , sharing 87.2–88.0 % sequence similarity with members of the closely related genera , and . Strain Gsoil 524 contained MK-7 as the predominant quinone, and iso-C, iso-C 3-OH, summed feature 3 (Cω7 and/or Cω6) and Cω5 as the major fatty acids. Strain Gsoil 524 could be distinguished from the other members of the family by a number of chemotaxonomic and phenotypic characteristics. The major polar lipids in strain Gsoil 524 were phosphatidylethanolamine and two unidentified polar lipids. Compared with the standard and reference strains unidentified sphingolipid was also found. Based on this polyphasic taxonomic analysis, strain Gsoil 524 represents a novel species within a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is Gsoil 524 ( = KACC 14636 = KCTC 22261 = LMG 24494).

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2016-03-01
2020-12-04
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