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Abstract

A novel endophytic actinobacterium, designated strain IP6SC6, was isolated from surface-sterilized bark of collected from Zhanjiang Mangrove Forest National Nature Reserve in Guangdong, China. Cells of strain IP6SC6 were Gram-stain-positive, aerobic, non-spore-forming, non-motile rods. Strain IP6SC6 grew at 20–42 °C (optimum, 37 °C), at pH 6.0–9.0 (optimum, pH 7.0) and in the presence of 0–8 % (w/v) NaCl (optimum, 0–2 %). Chemotaxonomic analyses showed that the isolate possessed -diaminopimelic acid as the diamino acid of the peptidoglycan, galactose and glucose as whole-cell sugars, and MK-8(H) as the predominant menaquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylinositol and an unknown lipid. The major fatty acids were iso-C, anteiso-C, anteiso-C and iso-C. The G+C content of the genomic DNA was 72.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain IP6SC6 belonged to the genus and shared the highest sequence similarity with NRRL B-24460 (96.97 %). On the basis of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain IP6SC6 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IP6SC6 ( = DSM 100020 = CGMCC 4.7300).

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2016-03-01
2019-10-23
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