gen. nov., sp. nov., a novel marine bacterium isolated from culture broth of a marine cyanobacterium Free

Abstract

A Gram-stain-negative, red, non-spore-forming, strictly aerobic bacterium, designated strain A4, was isolated from culture broth of a marine cyanobacterium. Cells were flexible rods with gliding motility. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain A4 formed a coherent cluster with members of the genera and , and represents a distinct lineage in the family . Thermotolerance and a distinctive cellular fatty acid profile could readily distinguish this isolate from any bacteria of the genera and with a validly published name. On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain A4 is suggested to represent a novel species in a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain is A4 ( = KCTC 42507 = CGMCC 1.15111).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000839
2016-02-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/2/1095.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000839&mimeType=html&fmt=ahah

References

  1. Barbeyron T., Kean K., Forterre P. 1984; DNA adenine methylation of GATC sequences appeared recently in the Escherichia coli lineage. J Bacteriol 160:586–590[PubMed]
    [Google Scholar]
  2. Barbeyron T., L'Haridon S., Corre E., Kloareg B., Potin P. 2001; Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [Cytophaga] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov. Int J Syst Evol Microbiol 51:985–997 [View Article][PubMed]
    [Google Scholar]
  3. Bernardet J. F., Nakagawa Y., Holmes B., Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070[PubMed]
    [Google Scholar]
  4. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [View Article][PubMed]
    [Google Scholar]
  5. Collins M. D. 1985; Analysis of isoprenoid quinones. Methods Microbiol 18:329–366 [View Article]
    [Google Scholar]
  6. Dong X.-Z., Cai M.-Y. 2001 Determinative Manual for Routine Bacteriology Beijiing: Scientific Press (English Translation);
    [Google Scholar]
  7. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  9. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. editors 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  10. Huo Y. Y., Xu L., Wang C. S., Yang J. Y., You H., Oren A., Xu X. W. 2013; Fabibacter pacificus sp. nov., a moderately halophilic bacterium isolated from seawater. Int J Syst Evol Microbiol 63:3710–3714 [View Article][PubMed]
    [Google Scholar]
  11. Kamekura M. 1993; Lipids of extreme halophiles. In The Biology of Halophilic Bacteria pp 135–161Edited by Vreeland R. H., Hochstein L. I. Boca Raton: CRC Press;
    [Google Scholar]
  12. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  13. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  14. Komagata K., Suzuki K. I. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  15. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175Edited by Stackebrandt E., Goodfellow M. Chichester, UK: Wiley;
    [Google Scholar]
  16. Lau S. C., Tsoi M. M., Li X., Plakhotnikova I., Dobretsov S., Wu M., Wong P. K., Pawlik J. R., Qian P. Y. 2006; Description of Fabibacter halotolerans gen. nov., sp. nov. and Roseivirga spongicola sp. nov., and reclassification of [Marinicola] seohaensis as Roseivirga seohaensis comb. nov. Int J Syst Evol Microbiol 56:1059–1065 [View Article][PubMed]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  18. Nedashkovskaya O. I., Kim S. B. 2010; Genus VI. Roseivirga. In Bergey's Manual of Systematic Bactriology pp 453–457Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B. 4 Baltimore: Williams & Wilkins;
    [Google Scholar]
  19. Nedashkovskaya O. I., Ludwig W. 2010; Family III. Flammeovirgaceae . In Bergey's Manual of Systematic Bactriology pp 442–447Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B. 4 Baltimore: Williams & Wilkins;
    [Google Scholar]
  20. Nedashkovskaya O. I., Kim S. B., Lee D. H., Lysenko A. M., Shevchenko L. S., Frolova G. M., Mikhailov V. V., Lee K. H., Bae K. S. 2005a; Roseivirga ehrenbergii gen. nov., sp. nov., a novel marine bacterium of the phylum ‘Bacteroidetes’, isolated from the green alga Ulva fenestrata . Int J Syst Evol Microbiol 55:231–234 [View Article][PubMed]
    [Google Scholar]
  21. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Park M. S., Mikhailov V. V., Bae K. S., Park H. Y. 2005b; Roseivirga echinicomitans sp. nov., a novel marine bacterium isolated from the sea urchin Strongylocentrotus intermedius, and emended description of the genus Roseivirga . Int J Syst Evol Microbiol 55:1797–1800 [View Article][PubMed]
    [Google Scholar]
  22. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Kalinovskaya N. I., Mikhailov V. V. 2008; Reclassification of Roseivirga seohaensis (Yoon et al. 2005) Lau et al. 2006 as a later synonym of Roseivirga ehrenbergii Nedashkovskaya et al. 2005 and emendation of the species description. Int J Syst Evol Microbiol 58:1194–1197 [View Article][PubMed]
    [Google Scholar]
  23. Pan J., Sun C., Wang R. J., Wu M. 2015; A novel species from seawater of the Pacific Ocean as Roseivirga marina sp. nov. Int J Syst Evol Microbiol [View Article][PubMed]
    [Google Scholar]
  24. Pruesse E., Peplies J., Glöckner F. O. 2012; SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 28:1823–1829 [View Article][PubMed]
    [Google Scholar]
  25. Reichenbach H., Kleinig H., Achenbach H. 1974; The pigments of Flexibacter elegans: novel and chemosystematically useful compounds. Arch Microbiol 101:131–144 [View Article][PubMed]
    [Google Scholar]
  26. Rzhetsky A., Nei M. 1992; A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9:945–967
    [Google Scholar]
  27. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  28. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–654Edited by Gerhardt P. Washington, D.C: American Society for Microbiology;
    [Google Scholar]
  29. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  30. Tindall B. J. 1990; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
    [Google Scholar]
  31. Wang G., Fan J., Wu H., Zhang X., Li G., Zhang H., Yang X., Ye F., Xiang W., Li X. 2013a; Nonhongiella spirulinensis gen. nov., sp. nov., a bacterium isolated from a cultivation pond of Spirulina platensis in Sanya, China. Antonie van Leeuwenhoek 104:933–939 [View Article][PubMed]
    [Google Scholar]
  32. Wang G., Wu H., Zhang X., Zhang H., Yang X., Tian X., Li J., Xiang W., Li X. 2013b; Aliidiomarina sanyensis sp. nov., a hexabromocyclododecane assimilating bacterium from the pool of Spirulina platensis cultivation, Sanya, China. Antonie van Leeuwenhoek 104:309–314 [View Article][PubMed]
    [Google Scholar]
  33. Wong S.-K., Park S., Lee J.-S., Lee K. C., Chiura H. X., Kogure K., Hamasaki K. 2015; Fabibacter misakiensis sp. nov., a marine bacterium isolated from coastal surface water. Int J Syst Evol Microbiol 65:3276–3280 [View Article][PubMed]
    [Google Scholar]
  34. Yoon J., Adachi K., Park S., Kasai H., Yokota A. 2011; Aureibacter tunicatorum gen. nov., sp. nov., a marine bacterium isolated from a coral reef sea squirt, and description of Flammeovirgaceae fam. nov. Int J Syst Evol Microbiol 61:2342–2347 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000839
Loading
/content/journal/ijsem/10.1099/ijsem.0.000839
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed