1887

Abstract

A Gram-stain-negative, motile, rod-shaped bacterium, designated MSSRFH1, was isolated from the rhizosphere of the mangrove, , in Pichavaram, Tamil Nadu, India. Phylogenetic analysis, based on the 16S rRNA gene sequence of MSSRFH1, indicated that it clustered in the genus and was most closely related to JCM18795 (96.7 % similarity). The 16S rRNA gene sequence similarity was < 96.5 % with other strains of species of the genus . The distinctiveness of strain MSSRFH1 was also shown by low similarities of its ( < 87 % similarity) and ( < 85 %) gene sequences with those of other members of the genus . Strain MSSRFH1 could tolerate NaCl concentrations of up to 30 % (w/v). The main fatty acids of MSSRFH1 included Cω7, C and Cω8. The polar lipids present included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified amino lipid and unidentified phospholipids. Comparison of BOX-PCR fingerprints indicated that MSSRFH1 showed a unique DNA profile and its genomic G+C content was 64 mol%. On the basis of the data presented, strain MSSRFH1 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is MSSRFH1 ( = KCTC 32998 = NBRC 110222).

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2016-02-01
2020-01-23
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References

  1. Aguilera M., Cabrera A., Incerti C., Fuentes S., Russell N. J., Ramos-Cormenzana A., Monteoliva-Sánchez M.. 2007; Chromohalobacter salarius sp. nov., a moderately halophilic bacterium isolated from a solar saltern in Cabo de Gata, Almeria, southern Spain. Int J Syst Evol Microbiol57:1238–1242 [CrossRef][PubMed]
    [Google Scholar]
  2. Anan'ina L. N., Plotnikova E. G., Gavrish E. Iu., Demakov V. A., Evtushenko L. I.. 2007; Salinicola socius gen. nov., sp. nov., a moderately halophilic bacterium from a naphthalene-utilizing microbial association. Microbiology76:324–330[PubMed][CrossRef]
    [Google Scholar]
  3. Arahal D. R., Vreeland R. H., Litchfield C. D., Mormile M. R., Tindall B. J., Oren A., Bejar V., Quesada E., Ventosa A.. 2007; Recommended minimal standards for describing new taxa of the family Halomonadaceae . Int J Syst Evol Microbiol57:2436–2446 [CrossRef][PubMed]
    [Google Scholar]
  4. Cappuccino J. G., Sherman N.. 1998; Microbiology: a Laboratory Manual, 5th edn. Menlo Park, CA: Benjamin/Cummings;
    [Google Scholar]
  5. de la Haba R. R., Márquez M. C., Papke R. T., Ventosa A.. 2012; Multilocus sequence analysis of the family Halomonadaceae . Int J Syst Evol Microbiol62:520–538 [CrossRef][PubMed]
    [Google Scholar]
  6. Fitch W. M.. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Biol20:406–416 [CrossRef]
    [Google Scholar]
  7. Huo Y.-Y., Meng F.-X., Xu L., Wang C. S., Xu X.-W.. 2013; Salinicola peritrichatus sp. nov., isolated from deep-sea sediment. Antonie van Leeuwenhoek104:55–62 [CrossRef][PubMed]
    [Google Scholar]
  8. Kates M.. 2010; Techniques of Lipidology, 3rd Revised Edition: Isolation, Analysis, and Identification of Lipids Newport Somerville Innovation, Limited;
    [Google Scholar]
  9. Kathiravan R., Jegan S., Ganga V., Prabavathy V. R., Tushar L., Sasikala Ch., Ramana ChV. 2013; Ciceribacter lividus gen. nov., sp. nov., isolated from rhizosphere soil of chick pea (Cicer arietinum L.). Int J Syst Evol Microbiol63:4484–4488 [CrossRef][PubMed]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  11. Kimura M.. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  12. Lane D.. 1991; 16S/23S rRNA sequencing. Nucleic Acid Techniques in Bacterial Systematics125–175
    [Google Scholar]
  13. Marmur J.. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol3:208–218 [CrossRef]
    [Google Scholar]
  14. Mata J. A., Martínez-Cánovas J., Quesada E., Béjar V.. 2002; A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol25:360–375 [CrossRef][PubMed]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B.. 1989; Precise measurement of the g+c content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39:159–167 [CrossRef]
    [Google Scholar]
  16. Raj P. S., Chakravarthy S. K., Ramaprasad E. V., Sasikala Ch., Ramana ChV. 2012; Phaeospirillum tilakii sp. nov., a phototrophic alphaproteobacterium isolated from aquatic sediments. Int J Syst Evol Microbiol62:1069–1074 [CrossRef][PubMed]
    [Google Scholar]
  17. Rameshkumar N., Nair S.. 2009; Isolation and molecular characterization of genetically diverse antagonistic, diazotrophic red-pigmented vibrios from different mangrove rhizospheres. FEMS Microbiol Ecol67:455–467 [CrossRef][PubMed]
    [Google Scholar]
  18. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  19. Sasser M.. 1990; Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  20. Tamura K., Dudley J., Nei M., Kumar S.. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol24:1596–1599 [CrossRef][PubMed]
    [Google Scholar]
  21. Tindall B. J., Rosselló-Móra R., Busse H. J., Ludwig W., Kämpfer P.. 2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol60:249–266 [CrossRef][PubMed]
    [Google Scholar]
  22. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A.. 1982; Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol128:1959–1968
    [Google Scholar]
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