1887

Abstract

A Gram-stain-negative, motile, rod-shaped bacterium, designated MSSRFH1, was isolated from the rhizosphere of the mangrove, , in Pichavaram, Tamil Nadu, India. Phylogenetic analysis, based on the 16S rRNA gene sequence of MSSRFH1, indicated that it clustered in the genus and was most closely related to JCM18795 (96.7 % similarity). The 16S rRNA gene sequence similarity was < 96.5 % with other strains of species of the genus . The distinctiveness of strain MSSRFH1 was also shown by low similarities of its ( < 87 % similarity) and ( < 85 %) gene sequences with those of other members of the genus . Strain MSSRFH1 could tolerate NaCl concentrations of up to 30 % (w/v). The main fatty acids of MSSRFH1 included Cω7, C and Cω8. The polar lipids present included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified amino lipid and unidentified phospholipids. Comparison of BOX-PCR fingerprints indicated that MSSRFH1 showed a unique DNA profile and its genomic G+C content was 64 mol%. On the basis of the data presented, strain MSSRFH1 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is MSSRFH1 ( = KCTC 32998 = NBRC 110222).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000837
2016-02-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/2/1074.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000837&mimeType=html&fmt=ahah

References

  1. Aguilera M., Cabrera A., Incerti C., Fuentes S., Russell N. J., Ramos-Cormenzana A., Monteoliva-Sánchez M. 2007; Chromohalobacter salarius sp. nov., a moderately halophilic bacterium isolated from a solar saltern in Cabo de Gata, Almeria, southern Spain. Int J Syst Evol Microbiol 57:1238–1242 [View Article][PubMed]
    [Google Scholar]
  2. Anan'ina L. N., Plotnikova E. G., Gavrish E. Iu., Demakov V. A., Evtushenko L. I. 2007; Salinicola socius gen. nov., sp. nov., a moderately halophilic bacterium from a naphthalene-utilizing microbial association. Microbiology 76:324–330[PubMed] [CrossRef]
    [Google Scholar]
  3. Arahal D. R., Vreeland R. H., Litchfield C. D., Mormile M. R., Tindall B. J., Oren A., Bejar V., Quesada E., Ventosa A. 2007; Recommended minimal standards for describing new taxa of the family Halomonadaceae . Int J Syst Evol Microbiol 57:2436–2446 [View Article][PubMed]
    [Google Scholar]
  4. Cappuccino J. G., Sherman N. 1998 Microbiology: a Laboratory Manual, 5th edn. Menlo Park, CA: Benjamin/Cummings;
    [Google Scholar]
  5. de la Haba R. R., Márquez M. C., Papke R. T., Ventosa A. 2012; Multilocus sequence analysis of the family Halomonadaceae . Int J Syst Evol Microbiol 62:520–538 [View Article][PubMed]
    [Google Scholar]
  6. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Biol 20:406–416 [View Article]
    [Google Scholar]
  7. Huo Y.-Y., Meng F.-X., Xu L., Wang C. S., Xu X.-W. 2013; Salinicola peritrichatus sp. nov., isolated from deep-sea sediment. Antonie van Leeuwenhoek 104:55–62 [View Article][PubMed]
    [Google Scholar]
  8. Kates M. 2010 Techniques of Lipidology, 3rd Revised Edition: Isolation, Analysis, and Identification of Lipids Newport Somerville Innovation, Limited;
    [Google Scholar]
  9. Kathiravan R., Jegan S., Ganga V., Prabavathy V. R., Tushar L., Sasikala Ch., Ramana ChV. 2013; Ciceribacter lividus gen. nov., sp. nov., isolated from rhizosphere soil of chick pea (Cicer arietinum L.). Int J Syst Evol Microbiol 63:4484–4488 [View Article][PubMed]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  11. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  12. Lane D. 1991; 16S/23S rRNA sequencing. Nucleic Acid Techniques in Bacterial Systematics125–175
    [Google Scholar]
  13. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  14. Mata J. A., Martínez-Cánovas J., Quesada E., Béjar V. 2002; A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 25:360–375 [View Article][PubMed]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the g+c content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  16. Raj P. S., Chakravarthy S. K., Ramaprasad E. V., Sasikala Ch., Ramana ChV. 2012; Phaeospirillum tilakii sp. nov., a phototrophic alphaproteobacterium isolated from aquatic sediments. Int J Syst Evol Microbiol 62:1069–1074 [View Article][PubMed]
    [Google Scholar]
  17. Rameshkumar N., Nair S. 2009; Isolation and molecular characterization of genetically diverse antagonistic, diazotrophic red-pigmented vibrios from different mangrove rhizospheres. FEMS Microbiol Ecol 67:455–467 [View Article][PubMed]
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  19. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  20. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  21. Tindall B. J., Rosselló-Móra R., Busse H. J., Ludwig W., Kämpfer P. 2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266 [View Article][PubMed]
    [Google Scholar]
  22. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1982; Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128:1959–1968
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000837
Loading
/content/journal/ijsem/10.1099/ijsem.0.000837
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error