1887

Abstract

A novel haloalkalitolerant, rod-shaped bacterium, designated strain YIM 4-4, was isolated from the surface water of the Dugerno lake, a haloalkaline lake in Inner Mongolia. The taxonomy of strain YIM 4-4 was investigated by a polyphasic approach. Strain YIM 4-4 was Gram-stain-negative, strictly aerobic, non-motile and formed red colonies. Optimal growth conditions were 28 °C, pH 8.0–11.0 and 0.5–2 % NaCl. The major respiratory quinone was menaquinone-7 (MK-7). The polar lipid profile was composed predominantly of phosphatidylethanolamine, six unidentified polar lipids, one phospholipid and one aminolipid. The predominant cellular fatty acids (>5 %) were iso-C, iso-CI/anteiso-CB, iso-CG, iso-C 3-OH, Cω7/Cω6 and iso-C. The genomic DNA G+C content was 43.0 mol%. 16S rRNA gene sequence analysis indicated that the members of the genera , , and of the family (phylum ) were the most closely related, with 16S rRNA gene sequence similarities ranging from 93.6 to 94.2 %. Other members of the family showed sequence similarities < 93.0 %. On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain YIM 4-4 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain is YIM 4-4 ( = CCTCC AB 2012966 = DSM 27929).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000836
2016-02-01
2020-04-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/2/1088.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000836&mimeType=html&fmt=ahah

References

  1. Albuquerque L., Tiago I., Nobre M. F., Veríssimo A., da Costa M. S.. 2013; Cecembia calidifontis sp. nov., isolated from a hot spring runoff, and emended description of the genus Cecembia. Int J Syst Evol Microbiol63:1431–1436 [CrossRef][PubMed]
    [Google Scholar]
  2. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J.. 1997; Gapped blast psi-blast: a new generation of protein database search programs. Nucleic Acids Res25:3389–3402 [CrossRef][PubMed]
    [Google Scholar]
  3. Anil Kumar P., Srinivas T. N. R, Madhu S., Manorama R., Shivaji S.. 2010a; Indibacter alkaliphilus gen. nov., sp. nov., an alkaliphilic bacterium isolated from a haloalkaline lake. Int J Syst Evol Microbiol60:721–726 [CrossRef][PubMed]
    [Google Scholar]
  4. Anil Kumar P., Srinivas T. N. R, Pavan Kumar P., Madhu S., Shivaji S.. 2010b; Nitritalea halalkaliphila gen. nov., sp. nov., an alkaliphilic bacterium of the family ‘Cyclobacteriaceae’, phylum Bacteroidetes. Int J Syst Evol Microbiol60:2320–2325 [CrossRef][PubMed]
    [Google Scholar]
  5. Anil Kumar P., Aravind R., Francis K., Bhumika V., Ritika C., Priyashanth P., Srinivas T,N.R.. 2012a; Shivajiella indica gen. nov., sp. nov., a marine bacterium of the family “Cyclobacteriaceae” with nitrate reducing activity. Syst Appl Microbiol35:320–325 [CrossRef][PubMed]
    [Google Scholar]
  6. Anil Kumar P., Srinivas T. N. R, Madhu S., Sravan R., Singh S., Naqvi S. W. A, Mayilraj S., Shivaji S.. 2012b; Cecembia lonarensis gen. nov., sp. nov., a haloalkalitolerant bacterium of the family Cyclobacteriaceae, isolated from a haloalkaline lake and emended descriptions of the genera Indibacter, Nitritalea and Belliella. Int J Syst Evol Microbiol62:2252–2258 [CrossRef][PubMed]
    [Google Scholar]
  7. Bhumika V., Srinivas T. N. R, Ravinder K., Anil Kumar P.. 2013; Mariniradius saccharolyticus gen. nov., sp. nov., a member of the family Cyclobacteriaceae isolated from marine aquaculture pond water, and emended descriptions of the genus Aquiflexum and Aquiflexum balticum. Int J Syst Evol Microbiol63:2088–2094 [CrossRef][PubMed]
    [Google Scholar]
  8. Bowman J. P., Nichols C. M., Gibson J. A. E. 2003; Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol53:1343–1355 [CrossRef][PubMed]
    [Google Scholar]
  9. Brettar I., Christen R., Höfle M. G.. 2004a; Belliella baltica gen. nov., sp. nov., a novel marine bacterium of the Cytophaga-Flavobacterium-Bacteroides group isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol54:65–70 [CrossRef][PubMed]
    [Google Scholar]
  10. Brettar I., Christen R., Höfle M. G.. 2004b; Aquiflexum balticum gen. nov., sp. nov., a novel marine bacterium of the Cytophaga-Flavobacterium-Bacteroides group isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol54:2335–2341 [CrossRef][PubMed]
    [Google Scholar]
  11. Cho J. C., Giovannoni S. J.. 2006; Pelagibaca bermudensis gen. nov., sp. nov., a novel marine bacterium within the Roseobacter clade in the order Rhodobacterales. Int J Syst Evol Microbiol56:855–859 [CrossRef][PubMed]
    [Google Scholar]
  12. Collins M. D., Jones D.. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Bacteriol48:459–470 [CrossRef]
    [Google Scholar]
  13. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  14. Cottrell M. T., Kirchman D. L.. 2000; Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization. Appl Environ Microbiol66:5116–5122 [CrossRef][PubMed]
    [Google Scholar]
  15. Cui X. L., Mao P. H., Zeng M., Li W. J., Zhang L. P., Xu L. H., Jiang C. L.. 2001; Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol51:357–363 [CrossRef][PubMed]
    [Google Scholar]
  16. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  17. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–791 [CrossRef]
    [Google Scholar]
  18. Fitch W. M.. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool20:406–416 [CrossRef]
    [Google Scholar]
  19. Garrity G. M., Holt J. G.. 2001; The road map to the Manual. In Bergey's Manual of Systematic Bacteriology, 2nd edn.vol 1 pp119–166Edited by Boone D. R., Castenholz R. W., Garrity G. M.. New York: Springer; [CrossRef]
    [Google Scholar]
  20. Gregersen T.. 1978; Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol5:123–127 [CrossRef]
    [Google Scholar]
  21. Hansen G. H., Sørheim R.. 1991; Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods13:231–241 [CrossRef]
    [Google Scholar]
  22. Höfle M. G.. 1992; Bacterioplankton community structure and dynamics after large-scale release of nonindigenous bacteria as revealed by low-molecular-weight-RNA analysis. Appl Environ Microbiol58:3387–3394[PubMed]
    [Google Scholar]
  23. Kämpfer P., Young C. C., Chen W. M., Rekha P. D., Fallschissel K., Lodders N., Chou J.-H., Shen F. T., Frischmann A., other authors. 2010; Fontibacter flavus gen. nov., sp. nov., a member of the family ‘Cyclobacteriaceae’, isolated from a hot spring. Int J Syst Evol Microbiol60:2066–2070 [CrossRef][PubMed]
    [Google Scholar]
  24. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  25. Kimura M.. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  26. Kovacs N.. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature178:703–704 [CrossRef][PubMed]
    [Google Scholar]
  27. Liu Y. P., Wang Y. X., Li Y. X., Feng F. Y., Liu H. R., Wang J.. 2012; Mongoliicoccus roseus gen. nov., sp. nov., an alkaliphilic bacterium isolated from a haloalkaline lake. Int J Syst Evol Microbiol62:2206–2212 [CrossRef][PubMed]
    [Google Scholar]
  28. Mesbah M., Premachandran U., Whitman W. B.. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39:159–167 [CrossRef]
    [Google Scholar]
  29. Minnikin D. E., Collins M. D., Goodfellow M.. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol47:87–95 [CrossRef]
    [Google Scholar]
  30. Murray R. G. E, Doetsch R. N., Robinow C. F.. 1994; Determinative and cytological light microscopy. Methods for general and molecular bacteriology1:22–41
    [Google Scholar]
  31. Nedashkovskaya O. I., Suzuki M., Vysotskii M. V., Mikhailov V. V.. 2003; Reichenbachia agariperforans gen. nov., sp. nov., a novel marine bacterium in the phylum Cytophaga-Flavobacterium-Bacteroides. Int J Syst Evol Microbiol53:81–85 [CrossRef][PubMed]
    [Google Scholar]
  32. Nedashkovskaya O. I., Kim S. B., Vancanneyt M., Lysenko A. M., Shin D. S., Park M. S., Lee K. H., Jung W. J., Kalinovskaya N. I., other authors. 2006; Echinicola pacifica gen. nov., sp. nov., a novel flexibacterium isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol56:953–958 [CrossRef][PubMed]
    [Google Scholar]
  33. Nedashkovskaya O. I., Ludwig W.. 2011; Family II. Cyclobacteriaceae fam. nov. (The Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes., Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes. In Bergey's Manual of Systematic Bacteriology, 2nd edn.vol 4 p423Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B.. New York: Springer;
    [Google Scholar]
  34. Raj H. D., Maloy S. R.. 1990; Proposal of Cyclobacterium marinus gen. nov., comb. nov., for a marine bacterium previously assigned to the genus Flectobacillus. Int J Syst Bacteriol40:337–347 [CrossRef]
    [Google Scholar]
  35. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  36. Sasser M.. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl20:16
    [Google Scholar]
  37. Schmidt M., Priemé A., Stougaard P.. 2006; Rhodonellum psychrophilum gen. nov., sp. nov., a novel psychrophilic and alkaliphilic bacterium of the phylum Bacteroidetes isolated from Greenland. Int J Syst Evol Microbiol56:2887–2892 [CrossRef][PubMed]
    [Google Scholar]
  38. Tamaoka J., Katayama-Fujimura Y., Kuraishi H.. 1983; Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol54:31–36 [CrossRef]
    [Google Scholar]
  39. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  40. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  41. Tian S. P., Wang Y. X., Hu B., Zhang X. X., Xiao W., Chen Y., Lai Y. H., Wen M. L., Cui X. L.. 2010; Litoribacter ruber gen. nov., sp. nov., an alkaliphilic, halotolerant bacterium isolated from a soda lake sediment. Int J Syst Evol Microbiol60:2996–3001 [CrossRef][PubMed]
    [Google Scholar]
  42. Yang C. X., Liu Y. P., Bao Q. H., Feng F. Y., Liu H. R., Zhang X. J., Zhao Y. L.. 2012; Mongoliitalea lutea gen. nov., sp. nov., an alkaliphilic, halotolerant bacterium isolated from a haloalkaline lake. Int J Syst Evol Microbiol62:647–653 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000836
Loading
/content/journal/ijsem/10.1099/ijsem.0.000836
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error