1887

Abstract

The taxonomic position of a novel actinobacterium, strain 169, isolated from a sample of Algerian Saharan soil, was determined using a polyphasic taxonomic approach. The aerial mycelium produced non-motile, round- to oval-shaped spores, with a smooth surface, which were sessile or carried by short sporophores. Chemotaxonomically, isolate 169 showed the same results as members of the genus , but madurose, the so far diagnostic sugar of the genus, was not detected in the whole-cell hydrolysate. Despite the absence of sporangia, the 16S rRNA gene sequence analysis confirmed that strain 169 was a member of the genus . Strain 169 was most closely related to NBRC 110047 (99.3 % 16S rRNA gene sequence similarity), which is the only non-sporangia-forming species reported among the genus. However, DNA–DNA hybridization studies with this species showed 60 % relatedness. Based upon genotypic and phenotypic data, a novel species, sp. nov., is proposed, with 169 ( = DSM 45455 = MTCC 11561 = CCUG 62974) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000829
2016-02-01
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/2/1034.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000829&mimeType=html&fmt=ahah

References

  1. Becker B., Lechevalier M. P., Gordon R. E., Lechevalier H. A.. ( 1964;). Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole-cell hydrolysates. Appl Microbiol 12: 421–423 [PubMed].
    [Google Scholar]
  2. Boudjella H., Bouti K., Zitouni A., Mathieu F., Lebrihi A., Sabaou N.. ( 2006;). Taxonomy and chemical characterization of antibiotics of Streptosporangium Sg 10 isolated from a Saharan soil. Microbiol Res 161: 288–298 [CrossRef] [PubMed].
    [Google Scholar]
  3. Cashion P., Holder-Franklin M. A., McCully J., Franklin M.. ( 1977;). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81: 461–466 [CrossRef] [PubMed].
    [Google Scholar]
  4. Couch J. N.. ( 1955;). A new genus and family of the Actinomycetales, with a revision of the genus Actinoplanes. J Elisha Mitchell Sci Soc 71: 148–155.
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12: 133–142 [CrossRef] [PubMed].
    [Google Scholar]
  6. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  7. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  8. Fitch W. M.. ( 1977;). On the problem of discovering the most parsimonious tree. Am Nat 111: 223–257 [CrossRef].
    [Google Scholar]
  9. Goodfellow M.. ( 1971;). Numerical taxonomy of some nocardioform bacteria. J Gen Microbiol 69: 33–80 [CrossRef] [PubMed].
    [Google Scholar]
  10. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N. ( 1974;). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24: 54–63 [CrossRef].
    [Google Scholar]
  11. Hayakawa M., Nonomura H.. ( 1987;). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65: 501–509 [CrossRef].
    [Google Scholar]
  12. Huss V. A. R, Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4: 184–192 [CrossRef] [PubMed].
    [Google Scholar]
  13. Intra B., Matsumoto A., Inahashi Y., O¯mura S., Panbangred W., Takahashi Y.. ( 2014;). Streptosporangium jomthongense sp. nov., an actinomycete isolated from rhizospheric soil and emendation of the genus Streptosporangium. Int J Syst Evol Microbiol 64: 2400–2406 [CrossRef] [PubMed].
    [Google Scholar]
  14. Jukes T. H., Cantor C. R.. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolismvol. 3, pp. 21–132. Edited by Munro H. N.. New York: Academic Press; [CrossRef].
    [Google Scholar]
  15. Kelly K. L., Judd D. B.. ( 1976;). Color. Universal Language and Dictionary of Names (National Bureau of Standards Special Publication 440) Washington, DC: Department of Commerce; [CrossRef].
    [Google Scholar]
  16. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  17. Kim M., Oh H. S., Park S. C., Chun J.. ( 2014;). Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 64: 346–351 [CrossRef] [PubMed].
    [Google Scholar]
  18. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111–120 [CrossRef] [PubMed].
    [Google Scholar]
  19. Kroppenstedt R. M.. ( 1982;). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5: 2359–2367 [CrossRef].
    [Google Scholar]
  20. Kroppenstedt R. M.. ( 1985;). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20, pp. 173–179. Edited by Goodfellow M., Minnikin D. E.. London: Academic Press;.
    [Google Scholar]
  21. Lechevalier M. P., Lechevalier H. A.. ( 1970;). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20: 435–443 [CrossRef].
    [Google Scholar]
  22. Marchal N., Bourdon J. L., Richard C. L.. ( 1987;). Les Milieux de Culture pour l'Isolement et l'Identification Biochimique des Bactéries., Paris: Doin;.
    [Google Scholar]
  23. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  24. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M.. ( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27: 104–117 [CrossRef].
    [Google Scholar]
  25. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  26. Quintana E., Goodfellow M.. ( 2012;). Genus I. Streptosporangium. . In Bergey's Manual of Systematic Bacteriology, 2nd edn.., vol. 5, pp. 1811–1825. Edited by Whitman W. B., Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Ludwig W., Suzuki K., Parte A.. New York: Springer;.
    [Google Scholar]
  27. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E.. ( 1996;). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46: 1088–1092 [CrossRef] [PubMed].
    [Google Scholar]
  28. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  29. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  30. Shirling E. B., Gottlieb D.. ( 1966;). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16: 313–340 [CrossRef].
    [Google Scholar]
  31. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  32. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673–4680 [CrossRef] [PubMed].
    [Google Scholar]
  33. Waksman S. A.. ( 1961;). The Actinomycetes, Classification, Identification and Description of Genera and Species, vol. 2. Baltimore, MD: Williams & Wilkins;.
    [Google Scholar]
  34. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D, Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C, Murray R. G. E, other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463–464 [CrossRef].
    [Google Scholar]
  35. Williams S. T., Goodfellow M., Alderson G.. ( 1989;). Genus Streptomyces Waksman and Henrici 1943, 339AL. . In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by Williams S. T., Sharpe M. E., Holt J. G.. Baltimore: Williams & Wilkins;.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000829
Loading
/content/journal/ijsem/10.1099/ijsem.0.000829
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error