1887

Abstract

The taxonomic positions of three Gram-stain-positive, aerobic strains, designated YIM 75722, 75726 and 75728, and isolated from a soil sample collected from Kurnool of Andhra Pradesh province, India, were assessed using a polyphasic approach. Growth was observed at pH 7.0–10.0 (optimum pH 7.0), 15–28 °C (optimum 28 °C) and 0–8 % (w/v) NaCl (grew without NaCl). Strains showed cylindrical spores with straight-chain morphology on aerial mycelium, but did not reveal sporangium-like structures or fragmentation of the substrate mycelium. Whole-cell hydrolysates of all strains contained galactose and ribose as the diagnostic sugars and -diaminopimelic acid as the diamino acid. The predominant menaquinone was MK-9(H); MK-9 (H) and MK-10 (H) were present in smaller amounts. The phospholipid pattern consisted mainly of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine. The major fatty acids were i-C, ai-C, i-C and ai-C. The genomic DNA G+C content was 68.0 mol%. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain YIM 75728 should be placed within the family , in which the strain formed a distinct lineage. The combination of phylogenetic analysis, phenotypic characteristics and chemotaxonomic data support the conclusion that strain YIM 75728 represents a novel species of a novel genus of the family for which the name gen. nov., sp. nov., is proposed. Strain YIM 75728 ( = DSM 45410 = CCTCC AA 209065) is the type strain of . Strain YIM 75728 was considered as the type strain over the other two strains based on the highest sequence read length of the strain.

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2016-02-01
2019-10-15
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