sp. nov., isolated from horse faeces Free

Abstract

The taxonomic position of five strains isolated from horse faeces, and which shared identical 16S rRNA gene sequences, were studied. Cells of all isolates are Gram-stain-negative, obligately aerobic and have a rod-shaped appearance. The strains show highest 16S rRNA gene sequence similarities to (98.3 %), (98.0 %), (97.9 %) and (97.9 %). Whole-genome sequencing of strain 114 and phylogeny reconstruction based on a core set of 1061 genes indicated that CIP 107468 was the closest relative among species of the genus for which whole genome sequences are available. The genomic DNA G+C content of strain 114 is 34.9 mol%, which is lower than any other value reported for the genus . The predominant polyamine is 1,3-diaminopropane, which is typical for the genus . The most abundant fatty acids are Cω7 and/or iso-C 2-OH (36 %) and C (28 %). The proportion of Cω9 (7 %) is distinctively low compared to most species of the genus. The major ubiquinone of strain 114 is Q-9. Microscopic studies revealed the presence of pili and the absence of flagella. The capability of all five strains to utilize -arabinose and gentisate as well as their lack of growth at temperatures of 41 °C and above provide sufficient criteria to distinguish the isolates from all species of the genus with validly published names. Based on these combined data, the five isolates represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 114 ( = DSM 27228 = CCUG 65204).

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2016-02-01
2024-03-28
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