1887

Abstract

A bacterial strain, designated TTM-24, was isolated from a freshwater river in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain TTM-24 were Gram-stain-negative, facultatively anaerobic, poly-β-hydroxybutyrate-accumulating, motile by a single polar flagellum, rod-shaped, with rods surrounded by a thick capsule and forming white-coloured colonies. Growth occurred at 15–37 °C (optimum, 25 °C), at pH 6.0–8.0 (optimum, pH 7.0) and with 0–1 % NaCl (optimum, 0.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TTM-24 belonged to the genus and was most closely related to ‘’ Npb-02 with sequence similarity of 97.1 %. Strain TTM-24 contained summed feature 3 (comprising C ω7 and/or C ω6) and C as the major fatty acids. The major respiratory quinone was Q-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminophospholipid and two uncharacterized phospholipids. The genomic DNA G+C content of strain TTM-24 was 67.4 mol%. The DNA–DNA hybridization value for strain TTM-24 with ‘’ Npb-02 was less than 45 %. On the basis of the phylogenetic inference and phenotypic data, strain TTM-24 should be classified as a novel species, for which the name sp. nov. is proposed. The type strain is TTM-24 ( = BCRC 80912 = KCTC 42742 = LMG 29003).

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2016-02-01
2020-03-28
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