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Abstract

A novel Gram-stain-positive, aerobic, endospore-forming, and rod-shaped strain designated 694 was isolated from surface-sterilized root tissue of a maize planted in the Fangshan District of Beijing, People's Republic of China. A polyphasic taxonomic study was performed on the new isolate. On the basis of 16S rRNA gene sequence similarity studies, this isolate belongs to the genus . High levels of 16S rRNA gene sequence similarity were found between strain 694 and DSM 30034 (98.5 %) and (98.1 %), respectively. However, the DNA–DNA hybridization values between strain 694 and its close relatives 16970 and CGMCC 1.10223 were 30.0 % and 36.7 % respectively. The DNA G+C content of strain 694 was determined to be 46.9 mol%. The predominant respiratory quinone was identified as menaquinone-7 and the polar lipid profile was found to be composed of the major lipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were found to be anteiso-C (42.1 %), iso-C (18.4 %), iso-C (11.2 %) and C (12.1 %). The results of physiological and biochemical tests and minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain 694 from the closely related species in the genus Strain 694 is concluded to represent a novel species within the genus , for which the name sp. nov. is proposed, with the type strain 694 ( = CGMCC 1.15286 = DSM 100762).

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2016-02-01
2021-10-16
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