1887

Abstract

Two Gram-staining-positive, endospore-forming, rod-shaped bacterial strains, designated strain SW109 and strain W006, were isolated from a seawater sample collected from the Indian Ocean. The strains were strictly aerobic, catalase- and oxidase-positive and motile by peritrichous flagella. The predominant cellular fatty acids (>10 %) were anteiso-C, iso-C and iso-C. The major menaquinone was menaquinone-7 (MK-7) and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The genomic DNA G+C content of strain SW109 and strain W006 was 46.3 and 46.1 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the two strains represented a novel member of the genus , showing the highest similarity with LMG 7121 and DSM 13666 (96.4 and 96.2 % sequence similarity, respectively). On the basis of phylogenetic inference and phenotypic characteristics, it is proposed that the two strains represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SW109 ( = CGMCC 1.12347 = DSM 100579).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000793
2016-02-01
2019-12-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/2/796.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000793&mimeType=html&fmt=ahah

References

  1. Albert R. A., Archambault J., Lempa M., Hurst B., Richardson C., Gruenloh S., Duran M., Worliczek H. L., Huber B. E., other authors. ( 2007;). Proposal of Viridibacillus gen. nov. and reclassification of Bacillus arvi, Bacillus arenosi and Bacillus neidei as Viridibacillus arvi gen. nov., comb. nov., Viridibacillus arenosi comb. nov. and Viridibacillus neidei comb. nov.. Int J Syst Evol Microbiol 57: 2729–2737 [CrossRef] [PubMed].
    [Google Scholar]
  2. Collins M. D.. ( 1985;). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E.. London: Academic Press;.
    [Google Scholar]
  3. Dong X.-Z., Cai M.-Y.. ( 2001;). Determinative Manual for Routine Bacteriology., Beijing: Scientific Press;.
    [Google Scholar]
  4. Embley T. M., Wait R.. ( 1994;). Structural lipids of eubacteria. . In Chemical Methods in Prokaryotic Systematics, pp. 121–161. Edited by Goodfellow M., O'Donnell A. G.. Chichester: Wiley;.
    [Google Scholar]
  5. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  6. Heyrman J., Vanparys B., Logan N. A., Balcaen A., Rodríguez-Díaz M., Felske A., De Vos P.. ( 2004;). Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands. Int J Syst Evol Microbiol 54: 47–57 [CrossRef] [PubMed].
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  8. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E.. ( 1988;). Fatty acids, antibiotic resistance and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 38: 358–361 [CrossRef].
    [Google Scholar]
  9. Lee Y.-J., Wagner I. D., Brice M. E., Kevbrin V. V., Mills G. L., Romanek C. S., Wiegel J.. ( 2005;). Thermosediminibacter oceani gen. nov., sp. nov. and Thermosediminibacter litoriperuensis sp. nov., new anaerobic thermophilic bacteria isolated from Peru Margin. Extremophiles 9: 375–383 [CrossRef] [PubMed].
    [Google Scholar]
  10. Li Z., Kawamura Y., Shida O., Yamagata S., Deguchi T., Ezaki T.. ( 2002;). Bacillus okuhidensis sp. nov., isolated from the Okuhida spa area of Japan. Int J Syst Evol Microbiol 52: 1205–1209 [PubMed].
    [Google Scholar]
  11. Lim J.-M., Jeon C. O., Lee S.-M., Lee J.-C., Xu L.-H., Jiang C.-L., Kim C.-J.. ( 2006;). Bacillus salarius sp. nov., a halophilic, spore-forming bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 56: 373–377 [CrossRef] [PubMed].
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  13. Miller L. T.. ( 1982;). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16: 584–586 [PubMed].
    [Google Scholar]
  14. Nicholson W. L., Munakata N., Horneck G., Melosh H. J., Setlow P.. ( 2000;). Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiol Mol Biol Rev 64: 548–572 [CrossRef] [PubMed].
    [Google Scholar]
  15. Nielsen P., Fritze D., Priest F. G.. ( 1995;). Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141: 1745–1761 [CrossRef].
    [Google Scholar]
  16. Priest F. G., Goodfellow M., Todd C.. ( 1988;). A numerical classification of the genus Bacillus. J Gen Microbiol 134: 1847–1882 [PubMed].
    [Google Scholar]
  17. Rhuland L. E., Work E., Denman R., Hoare D.. ( 1955;). The behavior of the isomers of diaminopimelic acid on paper chromatograms. J Am Chem Soc 77: 4844–4846 [CrossRef].
    [Google Scholar]
  18. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  19. Stackebrandt E., Ebers J.. ( 2006;). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33: 152–155.
    [Google Scholar]
  20. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24: 1596–1599 [CrossRef] [PubMed].
    [Google Scholar]
  21. Tindall B. J.. ( 1990;). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66: 199–202 [CrossRef].
    [Google Scholar]
  22. Tindall B. J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P.. ( 2010;). Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60: 249–266 [CrossRef] [PubMed].
    [Google Scholar]
  23. Wieser M., Worliczek H., Kämpfer P., Busse H.-J.. ( 2005;). Bacillus herbersteinensis sp. nov.. Int J Syst Evol Microbiol 55: 2119–2123 [CrossRef] [PubMed].
    [Google Scholar]
  24. Wu C., Lu X., Qin M., Wang Y., Ruan J.. ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16: 176–178.
    [Google Scholar]
  25. Xue Y., Ventosa A., Wang X., Ren P., Zhou P., Ma Y.. ( 2008;). Bacillus aidingensis sp. nov., a moderately halophilic bacterium isolated from Ai-Ding salt lake in China. Int J Syst Evol Microbiol 58: 2828–2832 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000793
Loading
/content/journal/ijsem/10.1099/ijsem.0.000793
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error