1887

Abstract

A strictly anaerobic bacterium, strain DLD10, was isolated from a biofilm that developed on a nanofiltration membrane treating anoxic groundwater using glycerol as substrate. Cells were straight to slightly curved rods 0.2–0.5 μm in diameter and 1–3 μm in length, non-motile and non-spore-forming. The optimum temperature and pH for growth were 30 °C and pH 7.0. Strain DLD10 was able to grow in the presence of 0.03–4.5 % (w/v) NaCl. Substrates utilized by strain DLD10 included glycerol and various carbohydrates (glucose, sucrose, fructose, mannose, arabinose, pectin, starch, xylan), which were mainly converted to ethanol, acetate, H and formate. Thiosulphate, sulphur and Fe(III) were used as electron acceptors, but sulphate, fumarate and nitrate were not. The predominant membrane fatty acids were C, iso-C and Cω8. The DNA G+C content was 36.4 mol%. Strain DLD10 belongs to the family and is distantly related to DSM 5832, DSM 15481 and CCUG 48729 (93 % 16S rRNA gene sequence similarity). Physiological characteristics and phylogenetic analysis indicated that strain DLD10 is a representative of a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is DLD10 ( = DSM 28816 = JCM 30818).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000791
2016-02-01
2020-04-02
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/2/774.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000791&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J.. 1990; Basic local alignment search tool. J Mol Biol215:403–410 [CrossRef][PubMed]
    [Google Scholar]
  2. Beyer F., Rietman B. M., Zwijnenburg A., Van den Brink P., Vrouwenvelder J. S., Jarzembowska M., Laurinonyte J., Stams A. J. M, Plugge C. M.. 2014; Long-term performance and fouling analysis of full-scale direct nanofiltration (NF) installations treating anoxic groundwater. J Membr Sci468:339–348 [CrossRef]
    [Google Scholar]
  3. Cline J. D.. 1969; Spectrophotometric determination of hydrogen sulfide in natural waters. Limnol Oceanogr14:454–458 [CrossRef]
    [Google Scholar]
  4. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. 1994; The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol44:812–826 [CrossRef]
    [Google Scholar]
  5. Cotta M. A., Whitehead T. R., Falsen E., Moore E., Lawson P. A.. 2009; Robinsoniella peoriensis gen. nov., sp. nov., isolated from a swine-manure storage pit and a human clinical source. Int J Syst Evol Microbiol59:150–155 [CrossRef][PubMed]
    [Google Scholar]
  6. Heilig H. G., Zoetendal E. G., Vaughan E. E., Marteau P., Akkermans A. D. L, de Vos W. M.. 2002; Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA. Appl Environ Microbiol68:114–123 [CrossRef][PubMed]
    [Google Scholar]
  7. Kämpfer P., Kroppenstedt R. M.. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol42:989–1005 [CrossRef]
    [Google Scholar]
  8. Lane D. J.. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp115–176Edited by Stackebrandt E., Goodfellow M.. Chichester: Wiley;
    [Google Scholar]
  9. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S., other authors. 2004; arb: a software environment for sequence data. Nucleic Acids Res32:1363–1371 [CrossRef][PubMed]
    [Google Scholar]
  10. Ludwig W., Schleifer K.-H., Whitman W. B.. 2009; Revised road map to the phylum Firmicutes. In Bergeys's Manual of Systematic Bacteriology, 2nd edn.vol 3 pp1–13Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K.-H., Whitman W. B.. New York: Springer; [CrossRef]
    [Google Scholar]
  11. Muyzer G., de Waal E. C., Uitterlinden A. G.. 1993; Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol59:695–700[PubMed]
    [Google Scholar]
  12. Nübel U., Engelen B., Felske A., Snaidr J., Wieshuber A., Amann R. I., Ludwig W., Backhaus H.. 1996; Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis. J Bacteriol178:5636–5643[PubMed]
    [Google Scholar]
  13. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  14. Sanguinetti C. J., Dias Neto E., Simpson A. J.. 1994; Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques17:914–921[PubMed]
    [Google Scholar]
  15. Sleat R., Mah R. A.. 1985; Clostridium populeti sp. nov., a cellulolytic species from a woody-biomass digestor. Int J Syst Bacteriol35:160–163 [CrossRef]
    [Google Scholar]
  16. Smibert R. M., Krieg N. R.. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp607–654Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  17. Stams A. J. M, Van Dijk J. B., Dijkema C., Plugge C. M.. 1993; Growth of syntrophic propionate-oxidizing bacteria with fumarate in the absence of methanogenic bacteria. Appl Environ Microbiol59:1114–1119[PubMed]
    [Google Scholar]
  18. Tamaoka J., Komagata K.. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett25:125–128 [CrossRef]
    [Google Scholar]
  19. van Gelder A. H., Aydin R., Alves M. M., Stams A. J. M. 2012; 1,3-Propanediol production from glycerol by a newly isolated Trichococcus strain. Microb Biotechnol5:573–578 [CrossRef][PubMed]
    [Google Scholar]
  20. Whitehead T. R., Cotta M. A., Collins M. D., Lawson P. A.. 2004; Hespellia stercorisuis gen. nov., sp. nov. and Hespellia porcina sp. nov., isolated from swine manure storage pits. Int J Syst Evol Microbiol54:241–245 [CrossRef][PubMed]
    [Google Scholar]
  21. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K. H., Ludwig W., Glöckner F. O., Rosselló-Móra R.. 2008; The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol31:241–250 [CrossRef][PubMed]
    [Google Scholar]
  22. Yutin N., Galperin M. Y.. 2013; A genomic update on clostridial phylogeny: Gram-negative spore formers and other misplaced clostridia. Environ Microbiol15:2631–2641[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000791
Loading
/content/journal/ijsem/10.1099/ijsem.0.000791
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error