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Abstract

A strictly anaerobic bacterium, strain DLD10, was isolated from a biofilm that developed on a nanofiltration membrane treating anoxic groundwater using glycerol as substrate. Cells were straight to slightly curved rods 0.2–0.5 μm in diameter and 1–3 μm in length, non-motile and non-spore-forming. The optimum temperature and pH for growth were 30 °C and pH 7.0. Strain DLD10 was able to grow in the presence of 0.03–4.5 % (w/v) NaCl. Substrates utilized by strain DLD10 included glycerol and various carbohydrates (glucose, sucrose, fructose, mannose, arabinose, pectin, starch, xylan), which were mainly converted to ethanol, acetate, H and formate. Thiosulphate, sulphur and Fe(III) were used as electron acceptors, but sulphate, fumarate and nitrate were not. The predominant membrane fatty acids were C, iso-C and Cω8. The DNA G+C content was 36.4 mol%. Strain DLD10 belongs to the family and is distantly related to DSM 5832, DSM 15481 and CCUG 48729 (93 % 16S rRNA gene sequence similarity). Physiological characteristics and phylogenetic analysis indicated that strain DLD10 is a representative of a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is DLD10 ( = DSM 28816 = JCM 30818).

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2016-02-01
2019-10-14
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