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Abstract

A halotolerant, Gram-positive bacterium (strain NE-14), which was isolated from sediment samples of the South China Sea, was subjected to a taxonomic study. Strain NE-14 grew well at wide temperature and pH ranges, 10.0–45.0 °C and pH 6–10, with an optimum at 30 °C and pH 8.0, respectively. Growth of strain NE-14 was observed at total salt concentrations of 0–10 % (w/v) with optimum at 2 % (w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparison indicated that the isolate belongs to the genus . Strain NE-14 was related most closely to LMG 18435, LMG 21833, SMC 4352-2 and LMG 21831 with 16S rRNA gene sequence similarities of 96.2, 95.9, 95.8 and 95.7 %, respectively. DNA–DNA hybridization between strain NE-14 and LMG 18435, LMG 21833, SMC 4352-2 and LMG 21831 gave reassociation values of about 27.4, 22.4, 16.4 and 15.9 %, respectively. The DNA G+C content of strain NE-14 was 39.2 mol%. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. The predominant cellular fatty acids of strain NE-14 were iso-C and anteiso-C. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phospholipid. Based on the phenotypic and phylogenetic characteristics, it is proposed that strain NE-14 be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NE-14 ( = DSM 28970 = JCM 30397).

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2016-02-01
2020-01-21
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