1887

Abstract

A Gram-reaction-variable, strictly aerobic, motile, rod-shaped bacterium, designated strain C4-5, was isolated from soil of a natural cave. Cells were oxidase- and catalase-positive and formed endospores in sporangia. The 16S rRNA gene sequence comparison showed that the organism formed a distinct clade within the genus and was most closely related to CKOBP-6 ( = KCTC 13623) (96.85 % 16S rRNA gene sequence similarity) followed by CAU 1005 ( = KCTC 33036) (94.82 %). The following chemotaxonomic features of strain C4-5 are typical for the genus : -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, MK-7 as the predominant menaquinone, the major fatty acids of anteiso-C, iso-C and C and the DNA G+C content of 54.8 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and four unidentified aminophospholipids. The phenotypic and phylogenetic data presented support that strain C4-5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is C4-5 ( = KCTC 33652 = DSM 100100).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000762
2016-02-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/2/598.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000762&mimeType=html&fmt=ahah

References

  1. Ash C. , Priest F. G. , Collins M. D. . ( 1993;). Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus . Antonie van Leeuwenhoek 64: 253–260 [CrossRef] [PubMed].
    [Google Scholar]
  2. Chou J.-H. , Lee J.-H. , Lin M.-C. , Chang P.-S. , Arun A. B. , Young C.-C. , Chen W.-M. . ( 2009;). Paenibacillus contaminans sp. nov., isolated from a contaminated laboratory plate. Int J Syst Evol Microbiol 59: 125–129 [CrossRef] [PubMed].
    [Google Scholar]
  3. Collins M. D. . ( 1985;). Analysis of isoprenoid quinones. Methods Microbiol 18: 329–366 [CrossRef].
    [Google Scholar]
  4. De Vos P. , Ludwig W. , Schleifer K. H. , Whitman W. B. . ( 2009;). Family IV. Paenibacillaceae fam. nov. . In Bergey's Manual of Systematic Bacteriology , 2nd edn.., vol. 3, p. 269. Edited by De Vos P. , Garrity G. M. , Jones D. , Krieg N. R. , Ludwig W. , Rainey F. , Schleifer K. H. , Whitman W. B. . New York: Springer;.
    [Google Scholar]
  5. Felsenstein J. . ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  6. Felsenstein J. . ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  7. Felsenstein J. . ( 2002;). PHYLIP (phylogeny inference package) version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.. .
  8. Fitch W. M. . ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20: 406–416 [CrossRef].
    [Google Scholar]
  9. Jukes T. H. , Cantor C. R. . ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21–132. Edited by Munro H. N. . New York: Academic Press;.[CrossRef]
    [Google Scholar]
  10. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  11. Kim J.-H. , Kang H. , Kim W. . ( 2014;). Paenibacillus doosanensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 64: 1271–1277 [CrossRef] [PubMed].
    [Google Scholar]
  12. Kroppenstedt R. M. . ( 1985;). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series), vol. 20, pp. 173–199. Edited by Goodfellow M. , Minnikin D. E. . New York: Academic Press;.
    [Google Scholar]
  13. Lane D. J. . ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E. , Goodfellow M. . Chichester: Wiley;.
    [Google Scholar]
  14. Lee S. D. , Lee D. W. . ( 2009;). Scopulibacillus darangshiensis gen. nov., sp. nov., isolated from rock. J Microbiol 47: 710–715 [CrossRef] [PubMed].
    [Google Scholar]
  15. Logan N. A. , Berge O. , Bishop A. H. , Busse H.-J. , De Vos P. , Fritze D. , Heyndrickx M. , Kämpfer P. , Rabinovitch L. , other authors . ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59: 2114–2121 [CrossRef] [PubMed].
    [Google Scholar]
  16. MacFaddin J. F. . ( 1980;). Biochemical Tests for Identification of Medical Bacteria , 2nd edn. Baltimore: Williams & Wilkins;.
    [Google Scholar]
  17. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  18. Minnikin D. E. , Patel P. V. , Alshamaony L. , Goodfellow M. . ( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27: 104–117 [CrossRef].
    [Google Scholar]
  19. Minnikin D. E. , O'Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  20. Priest F. G. . ( 2009;). Genus I. Paenibacillus Ash, Priest and Collins 1994. . In Bergey's Manual of Systematic Bacteriology , 2nd edn.., vol. 3, pp. 269–295. Edited by De Vos P. , Garrity G. M. , Jones D. , Krieg N. R. , Ludwig W. , Rainey F. A. , Schleifer K. H. , Whitman W. B. . New York: Springer;.
    [Google Scholar]
  21. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  22. Shida O. , Takagi H. , Kadowaki K. , Nakamura L. K. , Komagata K. . ( 1997;). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . Int J Syst Bacteriol 47: 289–298 [CrossRef] [PubMed].
    [Google Scholar]
  23. Staneck J. L. , Roberts G. D. . ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28: 226–231 [PubMed].
    [Google Scholar]
  24. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997;). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882 [CrossRef] [PubMed].
    [Google Scholar]
  25. Yao R. , Wang R. , Wang D. , Su J. , Zheng S. , Wang G. . ( 2014;). Paenibacillus selenitireducens sp. nov., a selenite-reducing bacterium isolated from a selenium mineral soil. Int J Syst Evol Microbiol 64: 805–811 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000762
Loading
/content/journal/ijsem/10.1099/ijsem.0.000762
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error