RT Journal Article SR Electronic(1) A1 Nogi, Yuichi A1 Mori, Kozue A1 Makita, Hiroko A1 Hatada, YujiYR 2016 T1 Thalassobius abyssi sp. nov., a marine bacterium isolated from cold-seep sediment JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 2 SP 574 OP 579 DO https://doi.org/10.1099/ijsem.0.000758 PB Microbiology Society, SN 1466-5034, AB A novel marine bacterial strain, designated JAMH 043T, was isolated from cold-seep sediment in Sagami Bay, Japan. Cells were Gram-stain-negative, rod-shaped, non-motile and aerobic chemo-organotrophs. The isolate grew optimally at 25 °C, at pH 7.0–7.5 and with 3 % (w/v) NaCl. The major respiratory quinone was ubiquinone-10 (Q-10). The predominant fatty acid was C18 : 1ω7c. On the basis of 16S rRNA gene sequence analysis, the isolated strain was closely affiliated to members of the genus Thalassobius in the class Alphaproteobacteria, and 16S rRNA gene sequence similarity of the novel isolate with the type strain of its closest related species, Thalassobius aestuarii JC2049T, was 98.4 %. The DNA G+C content of the novel strain was 58.0 mol%. The hybridization values for DNA–DNA relatedness between strain JAMH 043T and reference strains belonging to the genus Thalassobius were less than 14.1 ± 2.2 %. Based on differences in taxonomic characteristics, the isolated strain represents a novel species of the genus Thalassobius, for which the name Thalassobius abyssi sp. nov. is proposed. The type strain is JAMH 043T ( = JCM 30900T = DSM 100673T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000758