Ichthyobacterium seriolicida gen. nov., sp. nov., a member of the phylum ‘Bacteroidetes’, isolated from yellowtail fish (Seriola quinqueradiata) affected by bacterial haemolytic jaundice, and proposal of a new family, Ichthyobacteriaceae fam. nov.
A novel Gram-stain-negative, rod-shaped (0.3 × 4–6 μm), non-flagellated, aerobic strain with gliding motility, designated JBKA-6T, was isolated in 1991 from a yellowtail fish, Seriola quinqueradiata, showing symptoms of bacterial haemolytic jaundice. 16S rRNA gene sequence analysis showed that strain JBKA-6T was related most closely to members of the family Flavobacteriaceae in the phylum ‘Bacteroidetes’. Furthermore, based on gyrB gene sequence analysis, JBKA-6T was classified into a single clade within the order Flavobacteriales, which was distinct from the known clades of the families Flavobacteriaceae, Blattabacteriaceae and Cryomorphaceae. The predominant isoprenoid quinone was identified as MK-6 (97.9 %), and the major cellular fatty acids (>10 %) were C14 : 0 and iso-C15 : 0. The main polar lipids were phosphatidylethanolamine, three unidentified phospholipids, two unidentified aminophospholipids and two unidentified polar lipids. The DNA G+C content of JBKA-6T, as derived from its whole genome, was 33.4 mol%. The distinct phylogenetic position and phenotypic traits of strain JBKA-6T distinguish it from all other described species of the phylum ‘Bacteroidetes’, and therefore it was concluded that strain JBKA-6T represents a new member of the phylum ‘Bacteroidetes’, and the name Ichthyobacterium seriolicida gen. nov., sp. nov. is proposed. The type strain of Ichthyobacterium seriolicida is JBKA-6T ( = ATCC BAA-2465T = JCM 18228T). We also propose that Icthyobacterium gen. nov. is the type genus of a novel family, Ichthyobacteriaceae fam. nov.
BernardetJ. F., BowmanJ. P.2006; The genus Flavobacterium
. In The Prokaryotes: A Handbook on the Biology of Bacteria, 3rd edn. vol. 7 pp 481–531Edited byDworkinM., FalkowS., RosenbergE., SchleiferK. H., StackebrandtE. New York: Springer; [View Article]
BernardetJ. F., NakagawaY.2006; An introduction to the family Flavobacteriaceae
. In The Prokaryotes: A Handbook on the Biology of Bacteria, 3rd edn. vol 7 pp 455–480Edited byDworkinM., FalkowS., RosenbergE., SchleiferK. H., StackebrandtE. New York: Springer; [View Article]
BowmanJ. P.2006; The marine clade of the family Flavobacteriaceae: the genera Aequorivita, Arenibacter, Cellulophaga, Croceibacter, Formosa, Gelidibacter, Gillisia, Maribacter, Mesonia, Muricauda, Polaribacter, Psychroflexus, Psychroserpens, Robiginitalea, Salegentibacter, Tenacibaculum, Ulvibacter, Vitellibacter, and Zobellia
. In The Prokaryotes: A Handbook on the Biology of Bacteria, 3rd edn. vol 7 pp 677–694Edited byDworkinM., FalkowS., RosenbergE., SchleiferK. H., StackebrandtE. New York: Springer; [View Article]
ChunJ., LeeJ.-H., JungY., KimM., KimS., KimB. K., LimY.-W.2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
IidaT., SorimachiM.1994; Cultural characteristics of the bacterium causing jaundice of yellowtail, Seriola quinqueradiata
. Fish Pathol 29:25–28 [View Article]
JoosteP. J., BritzT. J., De HaastJ.1985; A numerical taxonomic study of Flavobacterium-Cytophaga strains from dairy sources. J Appl Bacteriol 59:311–323 [View Article][PubMed]
Katayama-FujimuraY., KomatsuY., KuraishiH., KanekoT.1984; Estimation of DNA base composition by high performance liquid chromatography of its nuclease P1 hydrolysate. Agric Biol Chem 48:3169–3172 [View Article]
LópezJ. R., Piñeiro-VidalM., García-LamasN., de la HerranR., NavasJ. I., Hachero-CruzadoI., SantosY.2010; First isolation of Tenacibaculum soleae from diseased cultured wedge sole, Dicologoglossa cuneata (Moreau), and brill, Scophthalmus rhombus (L.). J Fish Dis 33:273–278 [View Article][PubMed]
NishijimaM., SakaiM., SanoH.1997; Identification of isoprenoid quinones by frit-FAB liquid chromatography-mass spectrometry for the chemotaxonomy of microorganisms. J Microbiol Methods 28:113–122 [View Article]
ReichenbachH.1992; Flavobacteriaceae fam. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 41. Int J Syst Bacteriol 42:327–329 [View Article]
ThompsonJ. D., HigginsD. G., GibsonT. J.1994; clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
Ichthyobacterium seriolicida gen. nov., sp. nov., a member of the phylum ‘Bacteroidetes’, isolated from yellowtail fish (Seriola quinqueradiata) affected by bacterial haemolytic jaundice, and proposal of a new family, Ichthyobacteriaceae fam. nov.