1887

Abstract

A novel Gram-stain-negative, rod-shaped (0.3 × 4–6 μm), non-flagellated, aerobic strain with gliding motility, designated JBKA-6, was isolated in 1991 from a yellowtail fish, , showing symptoms of bacterial haemolytic jaundice. 16S rRNA gene sequence analysis showed that strain JBKA-6 was related most closely to members of the family in the phylum ‘’. Furthermore, based on gene sequence analysis, JBKA-6 was classified into a single clade within the order , which was distinct from the known clades of the families , and . The predominant isoprenoid quinone was identified as MK-6 (97.9 %), and the major cellular fatty acids (>10 %) were C and iso-C. The main polar lipids were phosphatidylethanolamine, three unidentified phospholipids, two unidentified aminophospholipids and two unidentified polar lipids. The DNA G+C content of JBKA-6, as derived from its whole genome, was 33.4 mol%. The distinct phylogenetic position and phenotypic traits of strain JBKA-6 distinguish it from all other described species of the phylum ‘’, and therefore it was concluded that strain JBKA-6 represents a new member of the phylum ‘’, and the name gen. nov., sp. nov. is proposed. The type strain of is JBKA-6 ( = ATCC BAA-2465 = JCM 18228). We also propose that gen. nov. is the type genus of a novel family, fam. nov.

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2016-02-01
2021-08-01
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References

  1. Bernardet J. F., Bowman J. P. 2006; The genus Flavobacterium . In The Prokaryotes: A Handbook on the Biology of Bacteria, 3rd edn. vol. 7 pp 481–531Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer; [View Article]
    [Google Scholar]
  2. Bernardet J. F., Nakagawa Y. 2006; An introduction to the family Flavobacteriaceae . In The Prokaryotes: A Handbook on the Biology of Bacteria, 3rd edn. vol 7 pp 455–480Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer; [View Article]
    [Google Scholar]
  3. Bowman J. P. 2006; The marine clade of the family Flavobacteriaceae: the genera Aequorivita, Arenibacter, Cellulophaga, Croceibacter, Formosa, Gelidibacter, Gillisia, Maribacter, Mesonia, Muricauda, Polaribacter, Psychroflexus, Psychroserpens, Robiginitalea, Salegentibacter, Tenacibaculum, Ulvibacter, Vitellibacter, and Zobellia . In The Prokaryotes: A Handbook on the Biology of Bacteria, 3rd edn. vol 7 pp 677–694Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer; [View Article]
    [Google Scholar]
  4. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  5. CLSI 2013 Performance Standards for Antimicrobial Disk Diffusion Susceptibility Tests; Approved Standard, 4th edn, Supplement VET01-A4 Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  6. Iida T., Sorimachi M. 1994; Cultural characteristics of the bacterium causing jaundice of yellowtail, Seriola quinqueradiata . Fish Pathol 29:25–28 [View Article]
    [Google Scholar]
  7. Jooste P. J., Britz T. J., De Haast J. 1985; A numerical taxonomic study of Flavobacterium-Cytophaga strains from dairy sources. J Appl Bacteriol 59:311–323 [View Article][PubMed]
    [Google Scholar]
  8. Katayama-Fujimura Y., Komatsu Y., Kuraishi H., Kaneko T. 1984; Estimation of DNA base composition by high performance liquid chromatography of its nuclease P1 hydrolysate. Agric Biol Chem 48:3169–3172 [View Article]
    [Google Scholar]
  9. Komagata K., Suzuki K. 1987; Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  10. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175Edited by Stackebrandt E., Goodfellow M. New York: Wiley;
    [Google Scholar]
  11. López J. R., Piñeiro-Vidal M., García-Lamas N., de la Herran R., Navas J. I., Hachero-Cruzado I., Santos Y. 2010; First isolation of Tenacibaculum soleae from diseased cultured wedge sole, Dicologoglossa cuneata (Moreau), and brill, Scophthalmus rhombus (L.). J Fish Dis 33:273–278 [View Article][PubMed]
    [Google Scholar]
  12. Maeno Y., Nakajima K., Sorimachi M., Inui Y. 1995; Pathophysiological studies of jaundiced yellowtail, Seriola quinqueradiata . Fish Pathol 30:7–14 [View Article] (in Japanese)
    [Google Scholar]
  13. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  14. Nishijima M., Sakai M., Sano H. 1997; Identification of isoprenoid quinones by frit-FAB liquid chromatography-mass spectrometry for the chemotaxonomy of microorganisms. J Microbiol Methods 28:113–122 [View Article]
    [Google Scholar]
  15. Piñeiro-Vidal M., Riaza A., Santos Y. 2008a; Tenacibaculum discolor sp. nov. and Tenacibaculum gallaicum sp. nov., isolated from sole (Solea senegalensis) and turbot (Psetta maxima) culture systems. Int J Syst Evol Microbiol 58:21–25 [View Article][PubMed]
    [Google Scholar]
  16. Piñeiro-Vidal M., Carballas C. G., Gómez-Barreiro O., Riaza A., Santos Y. 2008b; Tenacibaculum soleae sp. nov., isolated from diseased sole (Solea senegalensis Kaup). Int J Syst Evol Microbiol 58:881–885 [View Article][PubMed]
    [Google Scholar]
  17. Reichenbach H. 1992; Flavobacteriaceae fam. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 41. Int J Syst Bacteriol 42:327–329 [View Article]
    [Google Scholar]
  18. Sorimachi M., Maeno Y. 1993; Drug sensitivity of the causative bacterium of yellowtail jaundice. Fish Pathol 28:141–142 [View Article] (in Japanese)
    [Google Scholar]
  19. Sorimachi M., Maeno Y., Nakajima K., Inouye K., Inui Y. 1993; Causative agent of jaundice of yellowtail, Seriola quinqueradiata . Fish Pathol 28:119–124 (in Japanese) [View Article]
    [Google Scholar]
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  21. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  22. Wakabayashi H., Hikida M., Masumura K. 1986; Flexibacter maritimus sp. nov., a pathogen of marine fishes. Int J Syst Bacteriol 36:396–398 [View Article]
    [Google Scholar]
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