1887

Abstract

A novel Gram-stain-negative, rod-shaped (0.3 × 4–6 μm), non-flagellated, aerobic strain with gliding motility, designated JBKA-6, was isolated in 1991 from a yellowtail fish, , showing symptoms of bacterial haemolytic jaundice. 16S rRNA gene sequence analysis showed that strain JBKA-6 was related most closely to members of the family in the phylum ‘’. Furthermore, based on gene sequence analysis, JBKA-6 was classified into a single clade within the order , which was distinct from the known clades of the families , and . The predominant isoprenoid quinone was identified as MK-6 (97.9 %), and the major cellular fatty acids (>10 %) were C and iso-C. The main polar lipids were phosphatidylethanolamine, three unidentified phospholipids, two unidentified aminophospholipids and two unidentified polar lipids. The DNA G+C content of JBKA-6, as derived from its whole genome, was 33.4 mol%. The distinct phylogenetic position and phenotypic traits of strain JBKA-6 distinguish it from all other described species of the phylum ‘’, and therefore it was concluded that strain JBKA-6 represents a new member of the phylum ‘’, and the name gen. nov., sp. nov. is proposed. The type strain of is JBKA-6 ( = ATCC BAA-2465 = JCM 18228). We also propose that gen. nov. is the type genus of a novel family, fam. nov.

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2016-02-01
2020-10-01
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