1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 66, Issue 2

gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water Free

Abstract

A Gram-negative, heterotrophic, marine bacterium, designated strain TT1, was isolated from seawater collected from the shallow coastal region of Anping Harbour, Tainan, Taiwan. Cells grown in broth cultures were straight rods that were motile by means of a single polar flagellum. The isolate required NaCl for growth and exhibited optimal growth at 30–35 °C, 2–4 % NaCl and pH 7–8. Strain TT1 grew aerobically and was not capable of anaerobic growth by fermentation of glucose or other carbohydrates. Q-10 was the sole major isoprenoid quinone. Cellular fatty acids were dominated by Cω7 (47.5 %), C 2-OH (16.3 %) and Cω8 cyclo (10.6 %). The DNA G+C content was 56.4 mol%. Phylogeny based on 16S rRNA gene sequences showed that strain TT1 formed a distinct genus-level lineage in the family of the class and exhibited the highest sequence similarity with species of the genera (89.9–90.0 % 16S rRNA gene sequence similarity), (89.4–89.9 %) and (89.1–89.7 %). Strain TT1 could be distinguished from species of these phylogenetically closest genera based on differences in DNA G+C contents (56.4 mol% vs 60.0–68.0 mol%), fatty acid profiles and some physiological characteristics. On the basis of the polyphasic taxonomic data from this study, strain TT1 is considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is TT1 ( = JCM 18659 = BCRC 80493).

  • Published Online:
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000756
2016-02-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/2/592.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000756&mimeType=html&fmt=ahah

References

  1. Coenye T., Goris J., Spilker T., Vandamme P., LiPuma J. J. 2002; Characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of Inquilinus limosus gen. nov., sp. nov. J Clin Microbiol 40:2062–2069 [View Article][PubMed]
     [Google Scholar]
  2. Díaz-Cárdenas C., Patel B. K. C, Baena S. 2010; Tistlia consotensis gen. nov., sp. nov., an aerobic, chemoheterotrophic, free-living, nitrogen-fixing alphaproteobacterium, isolated from a Colombian saline spring. Int J Syst Evol Microbiol 60:1437–1443 [View Article][PubMed]
     [Google Scholar]
  3. Dong C., Lai Q., Chen L., Sun F., Shao Z., Yu Z. 2010; Oceanibaculum pacificum sp. nov., isolated from hydrothermal field sediment of the south-west Pacific Ocean. Int J Syst Evol Microbiol 60:219–222 [View Article][PubMed]
     [Google Scholar]
  4. Garrity G. M., Bell J. A., Lilburn T. 2005; Family I. Rhodospirillaceae Pfennig and Trüper 1971, 17AL . In Bergey's Manual of Systematic Bacteriology, 2nd edn. vol 2 The Proteobacteria, part C, The Alpha., Beta., Delta-, and Epsilon-Proteobacteria pp 1–40Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer; [View Article]
     [Google Scholar]
  5. Guyoneaud R., Mouné S., Eatock C., Bothorel V., Hirschler-Réa A., Willison J., Duran R., Liesack W., Herbert R.other authors 2002; Characterization of three spiral-shaped purple nonsulfur bacteria isolated from coastal lagoon sediments, saline sulfur springs, and microbial mats: emended description of the genus Roseospira and description of Roseospira marina sp. nov., Roseospira navarrensis sp. nov., and Roseospira thiosulfatophila sp. nov. Arch Microbiol 178:315–324 [View Article][PubMed]
     [Google Scholar]
  6. Imhoff J. F., Petri R., Süling J. 1998; Reclassification of species of the spiral-shaped phototrophic purple non-sulfur bacteria of the α-Proteobacteria: description of the new genera Phaeospirillum gen. nov., Rhodovibrio gen. nov., Rhodothalassium gen. nov. and Roseospira gen. nov. as well as transfer of Rhodospirillum fulvum to Phaeospirillum fulvum comb. nov., of Rhodospirillum molischianum to Phaeospirillum molischianum comb. nov., of Rhodospirillum salinarum to Rhodovibrio salinarum comb. nov., of Rhodospirillum sodomense to Rhodovibrio sodomensis comb. nov., of Rhodospirillum salexigens to Rhodothalassium salexigens comb. nov. and of Rhodospirillum mediosalinum to Roseospira mediosalina comb. nov. Int J Syst Bacteriol 48:793–798 [View Article][PubMed]
     [Google Scholar]
  7. Jean W. D., Shieh W. Y., Chiu H.-H. 2006; Pseudidiomarina taiwanensis gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the family Idiomarinaceae . Int J Syst Evol Microbiol 56:899–905 [View Article][PubMed]
     [Google Scholar]
  8. Kawasaki H., Hoshino Y., Kuraishi Y., Yamasato K. 1992; Rhodocista centenaria gen. nov., sp. nov., a cyst-forming anoxygenic photosynthetic bacterium and its phylogenetic position in the Proteobacteria alpha group. J Gen Appl Microbiol 38:541–551 [View Article]
     [Google Scholar]
  9. Lai Q., Yuan J., Wu C., Shao Z. 2009; Oceanibaculum indicum gen. nov., sp. nov., isolated from deep seawater of the Indian Ocean. Int J Syst Evol Microbiol 59:1733–1737 [View Article][PubMed]
     [Google Scholar]
  10. López-López A., Pujalte M. J., Benlloch S., Mata-Roig M., Rosselló-Mora R., Garay E., Rodríguez-Valera F. 2002; Thalassospira lucentensis gen. nov., sp. nov., a new marine member of the α-Proteobacteria . Int J Syst Evol Microbiol 52:1277–1283[PubMed]
     [Google Scholar]
  11. Mack E. E., Mandelco L., Woese C. R., Madigan M. T. 1993; Rhodospirillum sodomense, sp. nov., a Dead Sea Rhodospirillum species. Arch Microbiol 160:363–371 [View Article]
     [Google Scholar]
  12. Maszenan A. M., Seviour R. J., Patel B. K. C, Janssen P. H., Wanner J. 2005; Defluvicoccus vanus gen. nov., sp. nov., a novel Gram-negative coccus/coccobacillus in the ‘Alphaproteobacteria’ from activated sludge. Int J Syst Evol Microbiol 55:2105–2111 [View Article][PubMed]
     [Google Scholar]
  13. Nissen H., Dundas I. D. 1984; Rhodospirillum salinarum sp. nov., a halophilic photosynthetic bacterium isolated from a Portuguese saltern. Arch Microbiol 138:251–256 [View Article]
     [Google Scholar]
  14. Sasser M. 2001 Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
     [Google Scholar]
  15. Schleifer K. H., Schüler D., Spring S., Weizenegger M., Amann R., Ludwig W., Köhler M. 1991; The genus Magnetospirillum gen. nov. description of Magnetospirillum gryphiswaldense sp. nov. and transfer of Aquaspirillum magnetotacticum to Magnetospirillum magnetotacticum comb. nov. Syst Appl Microbiol 14:379–385 [View Article]
     [Google Scholar]
  16. Shi B. H., Arunpairojana V., Palakawong S., Yokota A. 2002; Tistrella mobilis gen nov., sp nov., a novel polyhydroxyalkanoate-producing bacterium belonging to α-Proteobacteria . J Gen Appl Microbiol 48:335–343 [View Article][PubMed]
     [Google Scholar]
  17. Shieh W. Y., Chen A.-L., Chiu H.-H. 2000; Vibrio aerogenes sp. nov., a facultatively anaerobic marine bacterium that ferments glucose with gas production. Int J Syst Evol Microbiol 50:321–329 [View Article][PubMed]
     [Google Scholar]
  18. Shieh W. Y., Lin Y.-T., Jean W. D. 2004; Pseudovibrio denitrificans gen. nov., sp. nov., a marine, facultatively anaerobic, fermentative bacterium capable of denitrification. Int J Syst Evol Microbiol 54:2307–2312 [View Article][PubMed]
     [Google Scholar]
  19. Shieh W. Y., Liu T. Y., Lin S. Y., Jean W. D., Chen J.-S. 2008; Simiduia agarivorans gen. nov., sp. nov., a marine, agarolytic bacterium isolated from shallow coastal water from Keelung, Taiwan. Int J Syst Evol Microbiol 58:895–900 [View Article][PubMed]
     [Google Scholar]
  20. Sizova M. V., Panikov N. S., Spiridonova E. M., Slobodova N. V., Tourova T. P. 2007; Novel facultative anaerobic acidotolerant Telmatospirillum siberiense gen. nov. sp. nov. isolated from mesotrophic fen. Syst Appl Microbiol 30:213–220 [View Article][PubMed]
     [Google Scholar]
  21. Skerman V. B. D, Sly L. I., Williamson M.-L. 1983; Conglomeromonas largomobilis gen. nov., sp. nov., a sodium-sensitive, mixed-flagellated organism from fresh waters. Int J Syst Bacteriol 33:300–308 [View Article]
     [Google Scholar]
  22. Sly L. I., Stackebrandt E. 1999; Description of Skermanella parooensis gen. nov., sp. nov. to accommodate Conglomeromonas largomobilis subsp. parooensis following the transfer of Conglomeromonas largomobilis subsp. largomobilis to the genus Azospirillum . Int J Syst Bacteriol 49:541–544 [View Article]
     [Google Scholar]
  23. Tarrand J. J., Krieg N. R., Döbereiner J. 1978; A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov. Can J Microbiol 24:967–980 [View Article][PubMed]
     [Google Scholar]
  24. Urios L., Michotey V., Intertaglia L., Lesongeur F., Lebaron P. 2008; Nisaea denitrificans gen. nov., sp. nov. and Nisaea nitritireducens sp. nov., two novel members of the class Alphaproteobacteria from the Mediterranean Sea. Int J Syst Evol Microbiol 58:2336–2341 [View Article][PubMed]
     [Google Scholar]
  25. Urios L., Michotey V., Intertaglia L., Lesongeur F., Lebaron P. 2010; Thalassobaculum salexigens sp. nov., a new member of the family Rhodospirillaceae from the NW Mediterranean Sea, and emended description of the genus Thalassobaculum . Int J Syst Evol Microbiol 60:209–213 [View Article][PubMed]
     [Google Scholar]
  26. Wang Y. X., Liu J. H., Zhang X. X., Chen Y. G., Wang Z. G., Chen Y., Li Q. Y., Peng Q., Cui X. L. 2009; Fodinicurvata sediminis gen. nov., sp. nov. and Fodinicurvata fenggangensis sp. nov., poly-β-hydroxybutyrate-producing bacteria in the family Rhodospirillaceae . Int J Syst Evol Microbiol 59:2575–2581 [View Article][PubMed]
     [Google Scholar]
  27. Xie C.-H., Yokota A. 2005; Azospirillum oryzae sp. nov., a nitrogen-fixing bacterium isolated from the roots of the rice plant Oryza sativa . Int J Syst Evol Microbiol 55:1435–1438 [View Article][PubMed]
     [Google Scholar]
  28. Yoon J. H., Kang S. J., Park S., Oh T. K. 2007; Caenispirillum bisanense gen. nov., sp. nov., isolated from sludge of a dye works. Int J Syst Evol Microbiol 57:1217–1221 [View Article][PubMed]
     [Google Scholar]
  29. Zhang G. I., Hwang C. Y., Cho B. C. 2008; Thalassobaculum litoreum gen. nov., sp. nov., a member of the family Rhodospirillaceae isolated from coastal seawater. Int J Syst Evol Microbiol 58:479–485 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000756
Loading
Tagaea marina gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water
Int J Syst Evol Microbiol 66, 592 (2016); https://doi.org/10.1099/ijsem.0.000756
/content/journal/ijsem/10.1099/ijsem.0.000756
/content/journal/ijsem/10.1099/ijsem.0.000756
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error