1887

Abstract

A novel actinobacterial strain, MUSC 78, was isolated from a mangrove soil collected from Peninsular Malaysia. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 78 represented a novel lineage within the class . Strain MUSC 78 formed a distinct clade in the family and was related most closely to members of the genera (98.3–96.8 % 16S rRNA gene sequence similarity), (98.2–96.8 %), (97.2 %), (97.0–95.3 %), (96.8 %), (96.6 %), (96.2–94.7 %), (96.1–94.8 %), (96.0–94.9 %) and (95.9 %). Cells were irregular rod-shaped or cocci and stained Gram-positive. The cell-wall peptidoglycan type was A3γ, with -diaminopimelic acid as the diagnostic diamino acid. The main cell-wall sugar was mannose and lower amounts of galactose and rhamnose were present. The predominant menaquinone was MK-8(H). The polar lipid profile consisted of phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipid. The predominant fatty acids were iso-C, anteiso-C and iso-C. The DNA G+C content was 73.1 mol%. Based on this polyphasic study, MUSC 78 exhibited phylogenetic and phenotypic differences from members of the genera of the family , and therefore a novel species of a new genus, gen. nov., sp. nov., is proposed. The type strain of is MUSC 78 ( = DSM 29621 = MCCC 1K00454 = NBRC 110749).

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2016-02-01
2021-07-26
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