1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 66, Issue 2

gen. nov., sp. nov., an actinobacterium of the family Free

Abstract

A novel actinobacterial strain, MUSC 78, was isolated from a mangrove soil collected from Peninsular Malaysia. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 78 represented a novel lineage within the class . Strain MUSC 78 formed a distinct clade in the family and was related most closely to members of the genera (98.3–96.8 % 16S rRNA gene sequence similarity), (98.2–96.8 %), (97.2 %), (97.0–95.3 %), (96.8 %), (96.6 %), (96.2–94.7 %), (96.1–94.8 %), (96.0–94.9 %) and (95.9 %). Cells were irregular rod-shaped or cocci and stained Gram-positive. The cell-wall peptidoglycan type was A3γ, with -diaminopimelic acid as the diagnostic diamino acid. The main cell-wall sugar was mannose and lower amounts of galactose and rhamnose were present. The predominant menaquinone was MK-8(H). The polar lipid profile consisted of phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipid. The predominant fatty acids were iso-C, anteiso-C and iso-C. The DNA G+C content was 73.1 mol%. Based on this polyphasic study, MUSC 78 exhibited phylogenetic and phenotypic differences from members of the genera of the family , and therefore a novel species of a new genus, gen. nov., sp. nov., is proposed. The type strain of is MUSC 78 ( = DSM 29621 = MCCC 1K00454 = NBRC 110749).

  • Published Online:
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000753
2016-02-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/2/554.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000753&mimeType=html&fmt=ahah

References

  1. Atlas R. M. 1993 Handbook of Microbiological MediaEdited by Parks L. C. Boca Raton: CRC Press;
     [Google Scholar]
  2. Carrillo P. G., Mardaraz C., Pitta-Alvarez S. I., Giulietti A. M. 1996; Isolation and selection of biosurfactant-producing bacteria. World J Microbiol Biotechnol 12:82–84 [View Article][PubMed]
     [Google Scholar]
  3. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
     [Google Scholar]
  4. Cerny G. 1978; Studies on aminopeptidase for the distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5:113–122 [View Article]
     [Google Scholar]
  5. Collins M. D., Dorsch M., Stackebrandt E. 1989; Transfer of Pimelobacter tumescens to Terrabacter gen. nov. as Terrabacter tumescens comb. nov. and of Pimelobacter jensenii to Nocardioides as Nocardioides jensenii comb. nov. Int J Syst Bacteriol 39:1–6 [View Article]
     [Google Scholar]
  6. Collins M. D., Routh J., Saraswathy A., Lawson P. A., Schumann P., Welinder-Olsson C., Falsen E. 2004; Arsenicicoccus bolidensis gen. nov., sp. nov., a novel actinomycete isolated from contaminated lake sediment. Int J Syst Evol Microbiol 54:605–608 [View Article][PubMed]
     [Google Scholar]
  7. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
     [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789 [View Article]
     [Google Scholar]
  9. Groth I., Schumann P., Martin K., Schuetze B., Augsten K., Kramer I., Stackebrandt E. 1999; Ornithinicoccus hortensis gen. nov., sp. nov., a soil actinomycete which contains l-ornithine. Int J Syst Bacteriol 49:1717–1724 [View Article][PubMed]
     [Google Scholar]
  10. Groth I., Schumann P., Weiss N., Schuetze B., Augsten K., Stackebrandt E. 2001; Ornithinimicrobium humiphilum gen. nov., sp. nov., a novel soil actinomycete with l-ornithine in the peptidoglycan. Int J Syst Evol Microbiol 51:81–87 [View Article][PubMed]
     [Google Scholar]
  11. Groth I., Schumann P., Schütze B., Augsten K., Stackebrandt E. 2002; Knoellia sinensis gen. nov., sp. nov. and Knoellia subterranea sp. nov., two novel actinobacteria isolated from a cave. Int J Syst Evol Microbiol 52:77–84 [View Article][PubMed]
     [Google Scholar]
  12. Hanada S., Liu W.-T., Shintani T., Kamagata Y., Nakamura K. 2002; Tetrasphaera elongata sp. nov., a polyphosphate-accumulating bacterium isolated from activated sludge. Int J Syst Evol Microbiol 52:883–887[PubMed]
     [Google Scholar]
  13. Hong K., Gao A. H., Xie Q. Y., Gao H., Zhuang L., Lin H. P., Yu H. P., Li J., Yao X. S., other authors. 2009; Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China. Mar Drugs 7:24–44 [View Article][PubMed]
     [Google Scholar]
  14. Jung S. Y., Kim H. S., Song J. J., Lee S. G., Oh T. K., Yoon J. H. 2006; Kribbia dieselivorans gen. nov., sp. nov., a novel member of the family Intrasporangiaceae . Int J Syst Evol Microbiol 56:2427–2432 [View Article][PubMed]
     [Google Scholar]
  15. Kageyama A., Takahashi Y., Seki T., Tomoda H., O¯mura S. 2005; Oryzihumus leptocrescens gen. nov., sp. nov. Int J Syst Evol Microbiol 55:2555–2559 [View Article][PubMed]
     [Google Scholar]
  16. Kageyama A., Takahashi Y., O¯mura S. 2007; Humihabitans oryzae gen. nov., sp. nov. Int J Syst Evol Microbiol 57:2163–2166 [View Article][PubMed]
     [Google Scholar]
  17. Kageyama A., Matsumoto A., O¯mura S., Takahashi Y. 2008a; Humibacillus xanthopallidus gen. nov., sp. nov. Int J Syst Evol Microbiol 58:1547–1551 [View Article][PubMed]
     [Google Scholar]
  18. Kageyama A., Haga T., Kasai H., Shizuri Y., O¯mura S., Takahashi Y. 2008b; Marihabitans asiaticum gen. nov., sp. nov., a meso-diaminopimelic acid-containing member of the family Intrasporangiaceae . Int J Syst Evol Microbiol 58:2429–2432 [View Article][PubMed]
     [Google Scholar]
  19. Kalakoutskii L. V., Kirillova I. P., Krassilnikov N. A. 1967; A new genus of the Actinomycetales, Intrasporangium gen. nov. J Gen Microbiol 48:79–85 [View Article]
     [Google Scholar]
  20. Kämpfer P., Terenius O., Lindh J. M., Faye I. 2006; Janibacter anophelis sp. nov., isolated from the midgut of Anopheles arabiensis . Int J Syst Evol Microbiol 56:389–392 [View Article][PubMed]
     [Google Scholar]
  21. Kates M. 1986 Techniques of Lipidology, 2nd edn. Amsterdam: Elsevier;
     [Google Scholar]
  22. Kelly K. L. 1964 Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
     [Google Scholar]
  23. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
     [Google Scholar]
  24. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
     [Google Scholar]
  25. Küster E., Williams S. T. 1964; Media for the isolation of streptomycetes. Nature 202:928–929 [View Article][PubMed]
     [Google Scholar]
  26. Lee S. D. 2006; Phycicoccus jejuensis gen. nov., sp. nov., an actinomycete isolated from seaweed. Int J Syst Evol Microbiol 56:2369–2373 [View Article][PubMed]
     [Google Scholar]
  27. Lee S. D., Lee D. W. 2007; Lapillicoccus jejuensis gen. nov., sp. nov., a novel actinobacterium of the family Intrasporangiaceae, isolated from stone. Int J Syst Evol Microbiol 57:2794–2798 [View Article][PubMed]
     [Google Scholar]
  28. Lee L.-H., Zainal N., Azman A.-S., Mutalib N. S., Hong K., Chan K.-G. 2014a; Mumia flava gen. nov., sp. nov., an actinobacterium of the family Nocardioidaceae . Int J Syst Evol Microbiol 64:1461–1467 [View Article][PubMed]
     [Google Scholar]
  29. Lee L.-H., Zainal N., Azman A.-S., Eng S.-K., Ab Mutalib N.-S., Yin W.-F., Chan K.-G. 2014b; Streptomyces pluripotens sp. nov., a bacteriocin-producing streptomycete that inhibits meticillin-resistant Staphylococcus aureus . Int J Syst Evol Microbiol 64:3297–3306 [View Article][PubMed]
     [Google Scholar]
  30. MacFaddin J. F. 2000 Biochemical Tests for Identification of Medical Bacteria, 3rd edn. Baltimore: Lippincott, Williams and Wilkins;
     [Google Scholar]
  31. Martin K., Schumann P., Rainey F. A., Schuetze B., Groth I. 1997; Janibacter limosus gen. nov., sp. nov., a new actinomycete with meso-diaminopimelic acid in the cell wall. Int J Syst Bacteriol 47:529–534 [View Article][PubMed]
     [Google Scholar]
  32. Maszenan A. M., Seviour R. J., Patel B. K. C, Schumann P., Burghardt J., Tokiwa Y., Stratton H. M. 2000; Three isolates of novel polyphosphate-accumulating gram-positive cocci, obtained from activated sludge, belong to a new genus, Tetrasphaera gen. nov., and description of two new species, Tetrasphaera japonica sp. nov. and Tetrasphaera australiensis sp. nov. Int J Syst Evol Microbiol 50:593–603 [View Article][PubMed]
     [Google Scholar]
  33. Meena B., Rajan L. A., Vinithkumar N. V., Kirubagaran R. 2013; Novel marine actinobacteria from emerald Andaman & Nicobar Islands: a prospective source for industrial and pharmaceutical byproducts. BMC Microbiol 13:145 [View Article][PubMed]
     [Google Scholar]
  34. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
     [Google Scholar]
  35. Montero-Barrientos M., Rivas R., Velázquez E., Monte E., Roig M. G. 2005; Terrabacter terrae sp. nov., a novel actinomycete isolated from soil in Spain. Int J Syst Evol Microbiol 55:2491–2495 [View Article][PubMed]
     [Google Scholar]
  36. Prauser H., Schumann P., Rainey F. A., Kroppenstedt R. M., Stackebrandt E. 1997; Terracoccus luteus gen. nov., sp. nov., an ll-diaminopimelic acid-containing coccoid actinomycete from soil. Int J Syst Bacteriol 47:1218–1224 [View Article][PubMed]
     [Google Scholar]
  37. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  38. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
     [Google Scholar]
  39. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477[PubMed]
     [Google Scholar]
  40. Schumann P. 2011; Peptidoglycan structure. Methods Microbiol 38:101–129 [View Article]
     [Google Scholar]
  41. Schumann P., Prauser H., Rainey F. A., Stackebrandt E., Hirsch P. 1997; Friedmanniella antarctica gen. nov., sp. nov., an ll-diaminopimelic acid-containing actinomycete from Antarctic sandstone. Int J Syst Bacteriol 47:278–283 [View Article][PubMed]
     [Google Scholar]
  42. Shieh W. Y., Chen Y.-W., Chaw S.-M., Chiu H.-H. 2003; Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol 53:479–484 [View Article][PubMed]
     [Google Scholar]
  43. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
     [Google Scholar]
  44. Stackebrandt E., Schumann P. 2000; Description of Bogoriellaceae fam. nov., Dermacoccaceae fam. nov., Rarobacteraceae fam. nov. and Sanguibacteraceae fam. nov. and emendation of some families of the suborder Micrococcineae . Int J Syst Evol Microbiol 50:1279–1285 [View Article][PubMed]
     [Google Scholar]
  45. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [View Article]
     [Google Scholar]
  46. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
     [Google Scholar]
  47. Takahashi Y., Matsumoto A., Seino A., Iwai Y., Omura S. 1996; Rare actinomycetes isolated from desert soils. Actinomycetologica 10:91–97 [View Article]
     [Google Scholar]
  48. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  49. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  50. Wang Z.-G., Wang Y.-X., Liu J.-H., Chen Y.-G., Zhang X.-X., Wen M.-L., Xu L.-H., Peng Q., Cui X.-L. 2009; Fodinibacter luteus gen. nov., sp. nov., an actinobacterium isolated from a salt mine. Int J Syst Evol Microbiol 59:2185–2190 [View Article][PubMed]
     [Google Scholar]
  51. Whiton R. S., Lau P., Morgan S. L., Gilbart J., Fox A. 1985; Modifications in the alditol acetate method for analysis of muramic acid and other neutral and amino sugars by capillary gas chromatography-mass spectrometry with selected ion monitoring. J Chromatogr A 347:109–120 [View Article][PubMed]
     [Google Scholar]
  52. Xiao C., Huang H., Ye J., Wu X., Zhu J., Zhan B., Bao S. 2011; Ornithinibacter aureus gen. nov., sp. nov., a novel member of the family Intrasporangiaceae . Int J Syst Evol Microbiol 61:659–664 [View Article][PubMed]
     [Google Scholar]
  53. Yi H., Schumann P., Sohn K., Chun J. 2004; Serinicoccus marinus gen. nov., sp. nov., a novel actinomycete with l-ornithine and l-serine in the peptidoglycan. Int J Syst Evol Microbiol 54:1585–1589 [View Article][PubMed]
     [Google Scholar]
  54. Zhi X. Y., Li W. J., Stackebrandt E. 2009; An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59:589–608 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000753
Loading
Monashia flava gen. nov., sp. nov., an actinobacterium of the family Intrasporangiaceae
Int J Syst Evol Microbiol 66, 554 (2016); https://doi.org/10.1099/ijsem.0.000753
/content/journal/ijsem/10.1099/ijsem.0.000753
/content/journal/ijsem/10.1099/ijsem.0.000753
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error