Egicoccus halophilus gen. nov., sp. nov., a halophilic, alkalitolerant actinobacterium and proposal of Egicoccaceae fam. nov. and Egicoccales ord. nov.
A novel Gram-stain-positive, non-motile, moderately halophilic and alkalitolerant actinobacterium, designated EGI 80432T, was isolated from a saline–alkaline soil of Xinjiang province, north-west China. Cells were non-endospore-forming cocci with a diameter of 0.5–0.8 μm. Strain EGI 80432T grew in the presence of 0–9 % (w/v) NaCl (optimum at 3–5 %), and also grew within the pH range 6.0–10.0 (optimum at pH 8.0–9.0) on marine 2216E medium. The peptidoglycan type was A1γ. The whole-cell hydrolysates contained glucose, galactose, mannose and three unknown sugars as major sugars. The predominant menaquinone was MK-9(H4). The major fatty acids were C17 : 1ω8c, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), C18 : 1ω9c and iso-C15 : 0 The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, one unknown phosphoglycolipid, three unknown phospholipids and four unknown polar lipids. The genomic DNA G+C content was 75.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain EGI 80432T clustered within the radius of the class Nitriliruptoria. Levels of sequence similarity between strain EGI 80432T and its phylogenetic neighbours Nitriliruptor alkaliphilus ANL-iso2T and Euzebya tangerina F10T were 94.1 and 88.1 %, respectively. Based on morphological, physiological and chemotaxonomic characteristics and phylogenetic analysis, a novel species of a new genus, Egicoccus halophilus gen. nov., sp. nov., is proposed, within the new family and new order Egicoccaceae fam. nov. and Egicoccales ord. nov. in the class Nitriliruptoria. The type strain of Egicoccus halophilus is EGI 80432T ( = CGMCC 1.14988T = KCTC 33612T).
CollinsM. D., PirouzT., GoodfellowM., MinnikinD. E.1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [View Article][PubMed]
KellyK. L.1964Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
MesbahM., PremachandranU., WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
MinnikinD. E., O'DonnellA. G., GoodfellowM., AldersonG., AthalyeM., SchaalK., ParlettJ. H.1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
SorokinD. Y., van PeltS., TourovaT. P., EvtushenkoL. I.2009; Nitriliruptor alkaliphilus gen. nov., sp. nov., a deep-lineage haloalkaliphilic actinobacterium from soda lakes capable of growth on aliphatic nitriles, and proposal of Nitriliruptoraceae fam. nov. and Nitriliruptorales ord. nov. Int J Syst Evol Microbiol 59:248–253 [View Article][PubMed]
StackebrandtE., RaineyF. A., Ward-RaineyN. L.1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [View Article]
TangS.-K., TianX.-P., ZhiX.-Y., CaiM., WuJ.-Y., YangL.-L., XuL.-H., LiW.-J.2008; Haloactinospora alba gen. nov., sp. nov., a halophilic filamentous actinomycete of the family Nocardiopsaceae
. Int J Syst Evol Microbiol 58:2075–2080 [View Article][PubMed]
TangS. K., WangY., ChenY., LouK., CaoL. L., XuL. H., LiW. J.2009; Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella
. Int J Syst Evol Microbiol 59:2025–2032 [View Article][PubMed]
WilliamsS. T., GoodfellowM., AldersonG., WellingtonE. M., SneathP. H., SackinM. J.1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813[PubMed]
XuP., LiW. J., TangS. K., ZhangY. Q., ChenG. Z., ChenH. H., XuL. H., JiangC. L.2005; Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153 [View Article][PubMed]
ZhiX. Y., LiW. J., StackebrandtE.2009; An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59:589–608 [View Article][PubMed]
Egicoccus halophilus gen. nov., sp. nov., a halophilic, alkalitolerant actinobacterium and proposal of Egicoccaceae fam. nov. and Egicoccales ord. nov.