RT Journal Article SR Electronic(1) A1 Phongsopitanun, Wongsakorn A1 Matsumoto, Atsuko A1 Inahashi, Yuki A1 Kudo, Takuji A1 Mori, Mihoko A1 Shiomi, Kazuro A1 Takahashi, Yoko A1 Tanasupawat, SomboonYR 2016 T1 Actinoplanes lichenis sp. nov., isolated from lichen JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 1 SP 468 OP 473 DO https://doi.org/10.1099/ijsem.0.000746 PB Microbiology Society, SN 1466-5034, AB A novel species of the genus Actinoplanes, strain LDG1-22T, for which we propose the name Actinoplanes lichenis sp. nov., was isolated from a lichen sample collected from tree bark in Thailand. The taxonomic position of the species has been described based on a polyphasic approach. Strain LDG1-22T produced irregular sporangia on agar media. It contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The major menaquinone was MK-9(H4); the polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and phosphatidylglycerol. Whole-cell hydrolysates contained ribose, glucose, mannose and small amounts of arabinose and xylose. The major cellular fatty acids were anteiso-C15 : 0 (31.2 %) and iso-C16 : 0 (14.2 %). Mycolic acids were absent. The G+C content was 73.6 %. 16S rRNA gene sequence analysis of strain LDG1-22T showed highest similarity (98.8 %) to Actinoplanes friuliensis DSM 45797T and it clustered with Actinoplanes nipponensis JCM 3264T and Actinoplanes missouriensis JCM 3121T in phylogenetic tree analysis. On the basis of the phenotypic characteristics and DNA–DNA relatedness, strain LDG1-22T could be distinguished from related species of the genus Actinoplanes and so represents a novel species of this genus. The type strain of Actinoplanes lichenis sp. nov. is LDG1-22T ( = JCM 30485T = TISTR 2343T = PCU 344T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000746