1887

Abstract

A novel species of the genus , strain LDG1-22, for which we propose the name sp. nov., was isolated from a lichen sample collected from tree bark in Thailand. The taxonomic position of the species has been described based on a polyphasic approach. Strain LDG1-22 produced irregular sporangia on agar media. It contained -diaminopimelic acid in the cell-wall peptidoglycan. The major menaquinone was MK-9(H); the polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and phosphatidylglycerol. Whole-cell hydrolysates contained ribose, glucose, mannose and small amounts of arabinose and xylose. The major cellular fatty acids were anteiso-C (31.2 %) and iso-C (14.2 %). Mycolic acids were absent. The G+C content was 73.6 %. 16S rRNA gene sequence analysis of strain LDG1-22 showed highest similarity (98.8 %) to DSM 45797 and it clustered with JCM 3264 and JCM 3121 in phylogenetic tree analysis. On the basis of the phenotypic characteristics and DNA–DNA relatedness, strain LDG1-22 could be distinguished from related species of the genus and so represents a novel species of this genus. The type strain of sp. nov. is LDG1-22 ( = JCM 30485 = TISTR 2343 = PCU 344).

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2016-01-01
2021-10-27
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References

  1. Arai T. 1975 Culture Media for Actinomycetes Tokyo, Japan: The Society for Actinomycetes;
    [Google Scholar]
  2. Aretz W., Meiwes J., Seibert G., Vobis G., Wink J. 2000; Friulimicins: novel lipopeptide antibiotics with peptidoglycan synthesis inhibiting activity from Actinoplanes friuliensis sp. nov. I. Taxonomic studies of the producing microorganism and fermentation. J Antibiot (Tokyo) 53:807–815 [View Article][PubMed]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [View Article][PubMed]
    [Google Scholar]
  4. Coronelli C., White R. J., Lancini G. C., Parenti F. 1975; Lipiarmycin, a new antibiotic from Actinoplanes. II. Isolation, chemical, biological and biochemical characterization. J Antibiot (Tokyo) 28:253–259 [View Article][PubMed]
    [Google Scholar]
  5. Couch J. N. 1950; Actinoplanes, a new genus of the Actinomycetales . J Elisha Mitchell Sci Soc 66:87–92
    [Google Scholar]
  6. Couch J. N. 1963; Some new genera and species of the Actinoplanaceae . J Elisha Mitchell Sci Soc 79:53–70
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  8. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  9. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  10. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  11. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63 [View Article]
    [Google Scholar]
  12. Hayakawa M., Nonomura H. 1987; Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65:501–509 [View Article]
    [Google Scholar]
  13. Inahashi Y., Matsumoto A., Danbara H., O¯mura S., Takahashi Y. 2010; Phytohabitans suffuscus gen. nov., sp. nov., an actinomycete of the family Micromonosporaceae isolated from plant roots. Int J Syst Evol Microbiol 60:2652–2658 [View Article][PubMed]
    [Google Scholar]
  14. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  15. Li B., Xie C., Yokota A. 2007; Nocardioides exalbidus sp. nov., a novel actinomycete isolated from lichen in Izu-Oshima island, Japan. Actinomycetologica 21:22–26 [View Article]
    [Google Scholar]
  16. Matsumoto A., Takahashi Y., Kudo T., Seino A., Iwai Y., Omura S. 2000; Actinoplanes capillaceus sp. nov., a new species of the genus Actinoplanes . Antonie van Leeuwenhoek 78:107–115 [View Article][PubMed]
    [Google Scholar]
  17. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  18. Parenti F., Coronelli C. 1979; Members of the genus Actinoplanes and their antibiotics. Annu Rev Microbiol 33:389–411 [View Article][PubMed]
    [Google Scholar]
  19. Parte A. C. 2014; LPSN—list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42:(D1)D613–D616 [View Article][PubMed]
    [Google Scholar]
  20. Saito H., Miura K. I. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619–629 [View Article][PubMed]
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  22. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  23. Shirling E. B., Gottlieb D. 1966; Method for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
    [Google Scholar]
  24. Somma S., Gastaldo L., Corti A. 1984; Teicoplanin, a new antibiotic from Actinoplanes teichomyceticus nov. sp. Antimicrob Agents Chemother 26:917–923 [View Article][PubMed]
    [Google Scholar]
  25. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
    [Google Scholar]
  26. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  27. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega 6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  28. Taylor H. D., Knoche L., Grauville W. C. 1958 Color Harmony Manual, 4th edn. Chicago: Container Corporation of America;
    [Google Scholar]
  29. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  30. Tomiyasu I. 1982; Mycolic acid composition and thermally adaptative changes in Nocardia asteroides . J Bacteriol 151:828–837[PubMed]
    [Google Scholar]
  31. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30:131–134 [CrossRef]
    [Google Scholar]
  32. Vobis G., Schäfer J., Kämpfer P. 2012; Genus III. Actinoplanes Couch 1950, 89AL emend. Stackebrandt and Kroppenstedt 1987, 112. In Bergey's Manual of Systematic Bacteriology, 2nd edn. vol. 5 pp 1058–1088Edited by Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M., Ludwig W., Suzuki K-i., Parte A., Whitman W. New York: Springer;
    [Google Scholar]
  33. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  34. Williams S. T., Cross T. 1971; Chapter XI Actinomycetes . Methods Microbiol 4:295–334 [View Article]
    [Google Scholar]
  35. Yamamura H., Ashizawa H., Nakagawa Y., Hamada M., Ishida Y., Otoguro M., Tamura T., Hayakawa M. 2011a; Actinomycetospora iriomotensis sp. nov., a novel actinomycete isolated from a lichen sample. J Antibiot (Tokyo) 64:289–292 [View Article][PubMed]
    [Google Scholar]
  36. Yamamura H., Ashizawa H., Nakagawa Y., Hamada M., Ishida Y., Otoguro M., Tamura T., Hayakawa M. 2011b; Actinomycetospora rishiriensis sp. nov., isolated from a lichen. Int J Syst Evol Microbiol 61:2621–2625 [View Article][PubMed]
    [Google Scholar]
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