1887

Abstract

Here, we report the culture and characterization of an alphaproteobacterium of the order , isolated from the gut of the honey bee . Strain PEB0122 shares >95 % 16S rRNA gene sequence similarity with species of the genus , a group of mammalian pathogens transmitted by bloodsucking arthropods. Phylogenetic analyses showed that PEB0122 and related strains from the honey bee gut form a sister clade of the genus . Optimal growth of strain PEB0122 was obtained on solid media supplemented with defibrinated sheep blood under microaerophilic conditions at 35–37 °C, which is consistent with the cultural characteristics of other species of the genus . Reduced growth of strain PEB0122 also occurred under aerobic conditions. The rod-shaped cells of strain PEB0122 had a mean length of 1.2–1.8 μm and revealed hairy surface structures. Strain PEB0122 was positive for catalase, cytochrome oxidase, urease and nitrate reductase. The fatty acid composition was comparable to those of other species of the genus , with palmitic acid (C) and isomers of 18- and 19-carbon chains being the most abundant. The genomic DNA G+C content of PEB0122 was determined to be about 45.5 mol%. The high 16S rRNA gene sequence similarity with species of and its close phylogenetic position suggest that strain PEB0122 represents a novel species within the genus , for which we propose the name sp. nov. The type strain is PEB0122 ( = NCIMB 14961 = DSM 29779).

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2016-01-01
2021-07-25
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