RT Journal Article SR Electronic(1) A1 Zhong, Zhi-Ping A1 Liu, Ying A1 Wang, Fang A1 Zhou, Yu-Guang A1 Liu, Hong-Can A1 Liu, Zhi-PeiYR 2016 T1 Lacimicrobium alkaliphilum gen. nov., sp. nov., a member of the family Alteromonadaceae isolated from a salt lake JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 1 SP 422 OP 429 DO https://doi.org/10.1099/ijsem.0.000735 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, facultatively aerobic bacterium, strain X13M-12T, was isolated from a salt lake (Lake Xiaochaidan) in the Qaidam basin, Qinghai Province, PR China. Cells of strain X13M-12T were slightly curved, rod-shaped, 0.5–0.8 μm wide and 1.2–2.3 μm long, and motile by means of a polar flagellum. Strain X13M-12T was catalase- and oxidase-positive. Growth was observed in the presence of 0–15.0 % (w/v) NaCl (optimum 3.0–5.0 %), and at 4–40 °C (optimum 25–30 °C) and pH 6.0–11.0 (optimum pH 8.5). Strain X13M-12T contained Q-8 as the sole respiratory quinone, and phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. The major cellular fatty acids (>10 % of totals) were C16 : 0, C16 : 1ω7c and/or C16 : 1ω6c, and C18 : 1ω7c and/or C18 : 1ω6c. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain X13M-12T belonged to the family Alteromonadaceae and formed a distinct lineage, showing low gene sequence similarities to closely related genera: Bowmanella, Aestuariibacter and Salinimonas (16S rRNA gene sequence similarities, 93.0–93.1 %, 92.3–93.1 % and 92.6–92.7 %, respectively). In addition, strain X13M-12T showed < 92.7 % gene sequence similarities to other species of the family Alteromonadaceae. The DNA G+C content of strain X13M-12T was 49 mol% (Tm). Based on the data presented above, strain X13M-12T is considered to represent a novel genus and species of the family Alteromonadaceae, for which the name Lacimicrobium alkaliphilum gen. nov., sp. nov. is proposed. The type strain is X13M-12T ( = CGMCC 1.12923T = KCTC 42674T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000735