1887

Abstract

A Gram-stain-negative, facultatively aerobic bacterium, strain X13M-12, was isolated from a salt lake (Lake Xiaochaidan) in the Qaidam basin, Qinghai Province, PR China. Cells of strain X13M-12 were slightly curved, rod-shaped, 0.5–0.8 μm wide and 1.2–2.3 μm long, and motile by means of a polar flagellum. Strain X13M-12 was catalase- and oxidase-positive. Growth was observed in the presence of 0–15.0 % (w/v) NaCl (optimum 3.0–5.0 %), and at 4–40 °C (optimum 25–30 °C) and pH 6.0–11.0 (optimum pH 8.5). Strain X13M-12 contained Q-8 as the sole respiratory quinone, and phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. The major cellular fatty acids (>10 % of totals) were C, Cω7 and/or Cω6, and Cω7 and/or Cω6. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain X13M-12 belonged to the family and formed a distinct lineage, showing low gene sequence similarities to closely related genera: , and (16S rRNA gene sequence similarities, 93.0–93.1 %, 92.3–93.1 % and 92.6–92.7 %, respectively). In addition, strain X13M-12 showed < 92.7 % gene sequence similarities to other species of the family . The DNA G+C content of strain X13M-12 was 49 mol% (Tm). Based on the data presented above, strain X13M-12 is considered to represent a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is X13M-12 ( = CGMCC 1.12923 = KCTC 42674).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000735
2016-01-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/1/422.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000735&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [View Article][PubMed]
    [Google Scholar]
  2. Baik K. S., Park Y. D., Seong C. N., Kim E. M., Bae K. S., Chun J. 2006; Glaciecola nitratireducens sp. nov., isolated from seawater. Int J Syst Evol Microbiol 56:2185–2188 [View Article][PubMed]
    [Google Scholar]
  3. Baumann L., Baumann P., Mandel M., Allen R. D. 1972; Taxonomy of aerobic marine eubacteria. J Bacteriol 110:402–429
    [Google Scholar]
  4. Bowman J. P., McCammon S. A., Brown J. L., McMeekin T. A. 1998; Glaciecola punicea gen. nov., sp. nov. and Glaciecola pallidula gen. nov., sp. nov.: psychrophilic bacteria from Antarctic sea-ice habitats. Int J Syst Bacteriol 48:1213–1222 [View Article]
    [Google Scholar]
  5. Chen L.-P., Xu H.-Y., Fu S.-Z., Fan H.-X., Liu Y.-H., Liu S.-J., Liu Z.-P. 2009a; Glaciecola lipolytica sp. nov., isolated from seawater near Tianjin city, China. Int J Syst Evol Microbiol 59:73–76 [View Article][PubMed]
    [Google Scholar]
  6. Chen Y.-G., Xiao H.-D., Tang S.-K., Zhang Y.-Q., Borrathybay E., Cui X.-L., Li W.-J., Liu Y.-Q. 2009b; Alteromonas halophila sp. nov., a new moderately halophilic bacterium isolated from a sea anemone. Antonie van Leeuwenhoek 96:259–266 [View Article][PubMed]
    [Google Scholar]
  7. Chiu H. H., Shieh W. Y., Lin S. Y., Tseng C. M., Chiang P. W., Wagner-Döbler I. 2007; Alteromonas tagae sp. nov. and Alteromonas simiduii sp. nov., mercury-resistant bacteria isolated from a Taiwanese estuary. Int J Syst Evol Microbiol 57:1209–1216 [View Article][PubMed]
    [Google Scholar]
  8. Dong X. Z., Cai M. Y. 2001 Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation);
    [Google Scholar]
  9. Du J., Dong C., Lai Q., Liu Y., Xie Y., Shao Z. 2015; Pseudobowmanella zhangzhouensis gen. nov., sp. nov., isolated from the surface freshwater of the Jiulong River in China. Antonie van Leeuwenhoek 107:741–748 [View Article][PubMed]
    [Google Scholar]
  10. Ekborg N. A., Gonzalez J. M., Howard M. B., Taylor L. E., Hutcheson S. W., Weiner R. M. 2005; Saccharophagus degradans gen. nov., sp. nov., a versatile marine degrader of complex polysaccharides. Int J Syst Evol Microbiol 55:1545–1549 [View Article][PubMed]
    [Google Scholar]
  11. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  12. Fitch W. M. 1971; Toward defining course of evolution-minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  13. Gauthier M. J., Lafay B., Christen R., Fernandez L., Acquaviva M., Bonin P., Bertrand J. C. 1992; Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. Int J Syst Bacteriol 42:568–576 [View Article][PubMed]
    [Google Scholar]
  14. González J. M., Mayer F., Moran M. A., Hodson R. E., Whitman W. B. 1997; Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol 47:369–376 [View Article][PubMed]
    [Google Scholar]
  15. Ivanova E. P., Mikhailov V. V. 2001; [A new family of Alteromonadaceae fam. nov., including the marine proteobacteria species Alteromonas, Pseudoalteromonas, Idiomarina i Colwellia]. Mikrobiologiia 70:15–23 (in Russian)
    [Google Scholar]
  16. Ivanova E. P., Bowman J. P., Lysenko A. M., Zhukova N. V., Gorshkova N. M., Sergeev A. F., Mikhailov V. V. 2005; Alteromonas addita sp. nov.. Int J Syst Evol Microbiol 55:1065–1068 [View Article][PubMed]
    [Google Scholar]
  17. Ivanova E. P., Ng H. J., Webb H. K., Kurilenko V. V., Zhukova N. V., Mikhailov V. V., Ponamoreva O. N., Crawford R. J. 2013; Alteromonas australica sp. nov., isolated from the Tasman Sea. Antonie van Leeuwenhoek 103:877–884 [View Article][PubMed]
    [Google Scholar]
  18. Ivanova E. P., López-Pérez M., Zabalos M., Nguyen S. H., Webb H. K., Ryan J., Lagutin K., Vyssotski M., Crawford R. J., Rodriguez-Valera F. 2015; Ecophysiological diversity of a novel member of the genus Alteromonas, and description of Alteromonas mediterranea sp. nov. Antonie van Leeuwenhoek 107:119–132 [View Article][PubMed]
    [Google Scholar]
  19. Jean W. D., Chen J. S., Lin Y. T., Shieh W. Y. 2006; Bowmanella denitrificans gen. nov., sp. nov., a denitrifying bacterium isolated from seawater from An-Ping Harbour, Taiwan. Int J Syst Evol Microbiol 56:2463–2467 [View Article][PubMed]
    [Google Scholar]
  20. Jean W. D., Huang S. P., Liu T. Y., Chen J. S., Shieh W. Y. 2009; Aliagarivorans marinus gen. nov., sp. nov. and Aliagarivorans taiwanensis sp. nov., facultatively anaerobic marine bacteria capable of agar degradation. Int J Syst Evol Microbiol 59:1880–1887 [View Article][PubMed]
    [Google Scholar]
  21. Jean W. D., Hsu C. Y., Huang S. P., Chen J. S., Lin S., Su M. H., Shieh W. Y. 2013; Reclassification of [Glaciecola] lipolytica and [Aestuariibacter] litoralis in Aliiglaciecola gen. nov., as Aliiglaciecola lipolytica comb. nov. and Aliiglaciecola litoralis comb. nov., respectively. Int J Syst Evol Microbiol 63:2859–2864 [View Article][PubMed]
    [Google Scholar]
  22. Jeon C. O., Lim J. M., Park D. J., Kim C. J. 2005; Salinimonas chungwhensis gen. nov., sp. nov., a moderately halophilic bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 55:239–243 [View Article][PubMed]
    [Google Scholar]
  23. Jin H. M., Jeong H. I., Jeon C. O. 2015; Aliiglaciecola aliphaticivorans sp. nov., an aliphatic hydrocarbon-degrading bacterium, isolated from a sea-tidal flat and emended description of the genus Aliiglaciecola Jean et al. 2013. Int J Syst Evol Microbiol 65:1550–1555 [View Article][PubMed]
    [Google Scholar]
  24. Kates M. 1986 Techniques of Lipidology, 2nd edn. Amsterdam: Elsevier;
    [Google Scholar]
  25. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  26. Kimura M. 1983 Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [View Article]
    [Google Scholar]
  27. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  28. Kurahashi M., Yokota A. 2004; Agarivorans albus gen. nov., sp. nov., a γ-proteobacterium isolated from marine animals. Int J Syst Evol Microbiol 54:693–697 [View Article][PubMed]
    [Google Scholar]
  29. Lai Q., Yuan J., Wang B., Sun F., Qiao N., Zheng T., Shao Z. 2009; Bowmanella pacifica sp. nov., isolated from a pyrene-degrading consortium. Int J Syst Evol Microbiol 59:1579–1582 [View Article][PubMed]
    [Google Scholar]
  30. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A., other authors. 2007; Clustal w and Clustal x version 2.0. Bioinformatics 23:2947–2948 [View Article][PubMed]
    [Google Scholar]
  31. Lim J. M., Jeon C. O., Lee J. C., Song S. M., Kim K. Y., Kim C. J. 2006; Marinimicrobium koreense gen. nov., sp. nov. and Marinimicrobium agarilyticum sp. nov., novel moderately halotolerant bacteria isolated from tidal flat sediment in Korea. Int J Syst Evol Microbiol 56:653–657 [View Article][PubMed]
    [Google Scholar]
  32. López-Pérez M., Rodriguez-Valera F. 2014; The family Alteromonadaceae . In The Prokaryotes pp 69–92Edited by Rosenberg E., DeLong E., Lory S., Stackebrandt E., Thompson F. Berlin Heidelberg: Springer;
    [Google Scholar]
  33. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
    [Google Scholar]
  34. Martínez-Checa F., Béjar V., Llamas I., Del Moral A., Quesada E. 2005; Alteromonas hispanica sp. nov., a polyunsaturated-fatty-acid-producing, halophilic bacterium isolated from Fuente de Piedra, southern Spain. Int J Syst Evol Microbiol 55:2385–2390 [View Article][PubMed]
    [Google Scholar]
  35. Matsuyama H., Hirabayashi T., Kasahara H., Minami H., Hoshino T., Yumoto I. 2006; Glaciecola chathamensis sp. nov., a novel marine polysaccharide-producing bacterium. Int J Syst Evol Microbiol 56:2883–2886 [View Article][PubMed]
    [Google Scholar]
  36. Matsuyama H., Minami H., Sakaki T., Kasahara H., Baba S., Ishimaru S., Hirota K., Yumoto I. 2015; Alteromonas gracilis sp. nov., a marine polysaccharide-producing bacterium. Int J Syst Evol Microbiol 65:1498–1503 [View Article][PubMed]
    [Google Scholar]
  37. Nokhal T.-H., Schlegel H. G. 1983; Taxonomic study of Paracoccus denitrificans . Int J Syst Bacteriol 33:26–37 [View Article]
    [Google Scholar]
  38. Park S., Yoon J. H. 2013; Glaciecola aquimarina sp. nov., a gammaproteobacterium isolated from coastal seawater. Antonie van Leeuwenhoek 103:1141–1148 [View Article][PubMed]
    [Google Scholar]
  39. Romanenko L. A., Zhukova N. V., Rohde M., Lysenko A. M., Mikhailov V. V., Stackebrandt E. 2003; Glaciecola mesophila sp. nov., a novel marine agar-digesting bacterium. Int J Syst Evol Microbiol 53:647–651 [View Article][PubMed]
    [Google Scholar]
  40. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  41. Shivaji S., Reddy G. S. 2014; Phylogenetic analyses of the genus Glaciecola: emended description of the genus Glaciecola, transfer of Glaciecola mesophila, G. agarilytica, G. aquimarina, G. arctica, G. chathamensis, G. polaris and G. psychrophila to the genus Paraglaciecola gen. nov. as Paraglaciecola mesophila comb. nov., P. agarilytica comb. nov., P. aquimarina comb. nov., P. arctica comb. nov., P. chathamensis comb. nov., P. polaris comb. nov. and P. psychrophila comb. nov., and description of Paraglaciecola oceanifecundans sp. nov., isolated from the Southern Ocean. Int J Syst Evol Microbiol 64:3264–3275 [View Article][PubMed]
    [Google Scholar]
  42. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  43. Tanaka N., Romanenko L. A., Frolova G. M., Mikhailov V. V. 2010; Aestuariibacter litoralis sp. nov., isolated from a sandy sediment of the Sea of Japan. Int J Syst Evol Microbiol 60:317–320 [View Article][PubMed]
    [Google Scholar]
  44. Teramoto M., Nishijima M. 2014; Agaribacter marinus gen. nov., sp. nov., an agar-degrading bacterium from surface seawater. Int J Syst Evol Microbiol 64:2416–2423 [View Article][PubMed]
    [Google Scholar]
  45. Urios L., Intertaglia L., Lesongeur F., Lebaron P. 2008; Haliea salexigens gen. nov., sp. nov., a member of the Gammaproteobacteria from the Mediterranean Sea. Int J Syst Evol Microbiol 58:1233–1237 [View Article][PubMed]
    [Google Scholar]
  46. Van Trappen S., Tan T. L., Yang J., Mergaert J., Swings J. 2004a; Glaciecola polaris sp. nov., a novel budding and prosthecate bacterium from the Arctic Ocean, and emended description of the genus Glaciecola . Int J Syst Evol Microbiol 54:1765–1771 [View Article][PubMed]
    [Google Scholar]
  47. Van Trappen S., Tan T. L., Yang J., Mergaert J., Swings J. 2004b; Alteromonas stellipolaris sp. nov., a novel, budding, prosthecate bacterium from Antarctic seas, and emended description of the genus Alteromonas . Int J Syst Evol Microbiol 54:1157–1163 [View Article][PubMed]
    [Google Scholar]
  48. Vandecandelaere I., Nercessian O., Segaert E., Achouak W., Mollica A., Faimali M., De Vos P., Vandamme P. 2008; Alteromonas genovensis sp. nov., isolated from a marine electroactive biofilm and emended description of Alteromonas macleodii Baumann et al. 1972 (approved lists 1980). Int J Syst Evol Microbiol 58:2589–2596 [View Article][PubMed]
    [Google Scholar]
  49. Vogel B. F., Venkateswaran K., Christensen H., Falsen E., Christiansen G., Gram L. 2000; Polyphasic taxonomic approach in the description of Alishewanella fetalis gen. nov., sp. nov., isolated from a human foetus. Int J Syst Evol Microbiol 50:1133–1142 [View Article][PubMed]
    [Google Scholar]
  50. Wang Y., Wang H., Liu J., Lai Q., Shao Z., Austin B., Zhang X.-H. 2010; Aestuariibacter aggregatus sp. nov., a moderately halophilic bacterium isolated from seawater of the Yellow Sea. FEMS Microbiol Lett 309:48–54[PubMed]
    [Google Scholar]
  51. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703[PubMed]
    [Google Scholar]
  52. Yi H., Bae K. S., Chun J. 2004; Aestuariibacter salexigens gen. nov., sp. nov. and Aestuariibacter halophilus sp. nov., isolated from tidal flat sediment, and emended description of Alteromonas macleodii . Int J Syst Evol Microbiol 54:571–576 [View Article][PubMed]
    [Google Scholar]
  53. Yong J. J., Park S. J., Kim H. J., Rhee S. K. 2007; Glaciecola agarilytica sp. nov., an agar-digesting marine bacterium from the East Sea, Korea. Int J Syst Evol Microbiol 57:951–953 [View Article][PubMed]
    [Google Scholar]
  54. Yoon J. H., Kim I. G., Kang K. H., Oh T. K., Park Y. H. 2003; Alteromonas marina sp. nov., isolated from sea water of the East Sea in Korea. Int J Syst Evol Microbiol 53:1625–1630 [View Article][PubMed]
    [Google Scholar]
  55. Yoon J. H., Yeo S. H., Oh T. K., Park Y. H. 2004; Alteromonas litorea sp. nov., a slightly halophilic bacterium isolated from an intertidal sediment of the Yellow Sea in Korea. Int J Syst Evol Microbiol 54:1197–1201 [View Article][PubMed]
    [Google Scholar]
  56. Yoon J. H., Kang S. J., Lee S. Y. 2012; Salinimonas lutimaris sp. nov., a polysaccharide-degrading bacterium isolated from a tidal flat. Antonie van Leeuwenhoek 101:803–810 [View Article][PubMed]
    [Google Scholar]
  57. Zhang D.-C., Yu Y., Chen B., Wang H.-X., Liu H.-C., Dong X.-Z., Zhou P.-J. 2006; Glaciecola psychrophila sp. nov., a novel psychrophilic bacterium isolated from the Arctic. Int J Syst Evol Microbiol 56:2867–2869 [View Article][PubMed]
    [Google Scholar]
  58. Zhang Y.-J., Zhang X.-Y., Mi Z.-H., Chen C.-X., Gao Z.-M., Chen X.-L., Yu Y., Chen B., Zhang Y.-Z. 2011; Glaciecola arctica sp. nov., isolated from Arctic marine sediment. Int J Syst Evol Microbiol 61:2338–2341 [View Article][PubMed]
    [Google Scholar]
  59. Zhong Z.-P., Liu Y., Liu H.-C., Wang F., Zhou Y.-G., Liu Z.-P. 2014; Roseibium aquae sp. nov., isolated from a saline lake. Int J Syst Evol Microbiol 64:2812–2818[PubMed] [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000735
Loading
/content/journal/ijsem/10.1099/ijsem.0.000735
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error