1887

Abstract

A Gram-stain-negative, facultatively aerobic bacterium, strain X13M-12, was isolated from a salt lake (Lake Xiaochaidan) in the Qaidam basin, Qinghai Province, PR China. Cells of strain X13M-12 were slightly curved, rod-shaped, 0.5–0.8 μm wide and 1.2–2.3 μm long, and motile by means of a polar flagellum. Strain X13M-12 was catalase- and oxidase-positive. Growth was observed in the presence of 0–15.0 % (w/v) NaCl (optimum 3.0–5.0 %), and at 4–40 °C (optimum 25–30 °C) and pH 6.0–11.0 (optimum pH 8.5). Strain X13M-12 contained Q-8 as the sole respiratory quinone, and phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. The major cellular fatty acids (>10 % of totals) were C, Cω7 and/or Cω6, and Cω7 and/or Cω6. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain X13M-12 belonged to the family and formed a distinct lineage, showing low gene sequence similarities to closely related genera: , and (16S rRNA gene sequence similarities, 93.0–93.1 %, 92.3–93.1 % and 92.6–92.7 %, respectively). In addition, strain X13M-12 showed < 92.7 % gene sequence similarities to other species of the family . The DNA G+C content of strain X13M-12 was 49 mol% (Tm). Based on the data presented above, strain X13M-12 is considered to represent a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is X13M-12 ( = CGMCC 1.12923 = KCTC 42674).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000735
2016-01-01
2020-01-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/1/422.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000735&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J.. 1990; Basic local alignment search tool. J Mol Biol215:403–410 [CrossRef][PubMed]
    [Google Scholar]
  2. Baik K. S., Park Y. D., Seong C. N., Kim E. M., Bae K. S., Chun J.. 2006; Glaciecola nitratireducens sp. nov., isolated from seawater. Int J Syst Evol Microbiol56:2185–2188 [CrossRef][PubMed]
    [Google Scholar]
  3. Baumann L., Baumann P., Mandel M., Allen R. D.. 1972; Taxonomy of aerobic marine eubacteria. J Bacteriol110:402–429
    [Google Scholar]
  4. Bowman J. P., McCammon S. A., Brown J. L., McMeekin T. A.. 1998; Glaciecola punicea gen. nov., sp. nov. and Glaciecola pallidula gen. nov., sp. nov.: psychrophilic bacteria from Antarctic sea-ice habitats. Int J Syst Bacteriol48:1213–1222 [CrossRef]
    [Google Scholar]
  5. Chen L.-P., Xu H.-Y., Fu S.-Z., Fan H.-X., Liu Y.-H., Liu S.-J., Liu Z.-P.. 2009a; Glaciecola lipolytica sp. nov., isolated from seawater near Tianjin city, China. Int J Syst Evol Microbiol59:73–76 [CrossRef][PubMed]
    [Google Scholar]
  6. Chen Y.-G., Xiao H.-D., Tang S.-K., Zhang Y.-Q., Borrathybay E., Cui X.-L., Li W.-J., Liu Y.-Q.. 2009b; Alteromonas halophila sp. nov., a new moderately halophilic bacterium isolated from a sea anemone. Antonie van Leeuwenhoek96:259–266 [CrossRef][PubMed]
    [Google Scholar]
  7. Chiu H. H., Shieh W. Y., Lin S. Y., Tseng C. M., Chiang P. W., Wagner-Döbler I.. 2007; Alteromonas tagae sp. nov. and Alteromonas simiduii sp. nov., mercury-resistant bacteria isolated from a Taiwanese estuary. Int J Syst Evol Microbiol57:1209–1216 [CrossRef][PubMed]
    [Google Scholar]
  8. Dong X. Z., Cai M. Y.. 2001; Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation);
    [Google Scholar]
  9. Du J., Dong C., Lai Q., Liu Y., Xie Y., Shao Z.. 2015; Pseudobowmanella zhangzhouensis gen. nov., sp. nov., isolated from the surface freshwater of the Jiulong River in China. Antonie van Leeuwenhoek107:741–748 [CrossRef][PubMed]
    [Google Scholar]
  10. Ekborg N. A., Gonzalez J. M., Howard M. B., Taylor L. E., Hutcheson S. W., Weiner R. M.. 2005; Saccharophagus degradans gen. nov., sp. nov., a versatile marine degrader of complex polysaccharides. Int J Syst Evol Microbiol55:1545–1549 [CrossRef][PubMed]
    [Google Scholar]
  11. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  12. Fitch W. M.. 1971; Toward defining course of evolution-minimum change for a specific tree topology. Syst Zool20:406–416 [CrossRef]
    [Google Scholar]
  13. Gauthier M. J., Lafay B., Christen R., Fernandez L., Acquaviva M., Bonin P., Bertrand J. C.. 1992; Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. Int J Syst Bacteriol42:568–576 [CrossRef][PubMed]
    [Google Scholar]
  14. González J. M., Mayer F., Moran M. A., Hodson R. E., Whitman W. B.. 1997; Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol47:369–376 [CrossRef][PubMed]
    [Google Scholar]
  15. Ivanova E. P., Mikhailov V. V.. 2001; [A new family of Alteromonadaceae fam. nov., including the marine proteobacteria species Alteromonas, Pseudoalteromonas, Idiomarina i Colwellia]. Mikrobiologiia70:15–23 (in Russian)
    [Google Scholar]
  16. Ivanova E. P., Bowman J. P., Lysenko A. M., Zhukova N. V., Gorshkova N. M., Sergeev A. F., Mikhailov V. V.. 2005; Alteromonas addita sp. nov.. Int J Syst Evol Microbiol55:1065–1068 [CrossRef][PubMed]
    [Google Scholar]
  17. Ivanova E. P., Ng H. J., Webb H. K., Kurilenko V. V., Zhukova N. V., Mikhailov V. V., Ponamoreva O. N., Crawford R. J.. 2013; Alteromonas australica sp. nov., isolated from the Tasman Sea. Antonie van Leeuwenhoek103:877–884 [CrossRef][PubMed]
    [Google Scholar]
  18. Ivanova E. P., López-Pérez M., Zabalos M., Nguyen S. H., Webb H. K., Ryan J., Lagutin K., Vyssotski M., Crawford R. J., Rodriguez-Valera F.. 2015; Ecophysiological diversity of a novel member of the genus Alteromonas, and description of Alteromonas mediterranea sp. nov. Antonie van Leeuwenhoek107:119–132 [CrossRef][PubMed]
    [Google Scholar]
  19. Jean W. D., Chen J. S., Lin Y. T., Shieh W. Y.. 2006; Bowmanella denitrificans gen. nov., sp. nov., a denitrifying bacterium isolated from seawater from An-Ping Harbour, Taiwan. Int J Syst Evol Microbiol56:2463–2467 [CrossRef][PubMed]
    [Google Scholar]
  20. Jean W. D., Huang S. P., Liu T. Y., Chen J. S., Shieh W. Y.. 2009; Aliagarivorans marinus gen. nov., sp. nov. and Aliagarivorans taiwanensis sp. nov., facultatively anaerobic marine bacteria capable of agar degradation. Int J Syst Evol Microbiol59:1880–1887 [CrossRef][PubMed]
    [Google Scholar]
  21. Jean W. D., Hsu C. Y., Huang S. P., Chen J. S., Lin S., Su M. H., Shieh W. Y.. 2013; Reclassification of [Glaciecola] lipolytica and [Aestuariibacter] litoralis in Aliiglaciecola gen. nov., as Aliiglaciecola lipolytica comb. nov. and Aliiglaciecola litoralis comb. nov., respectively. Int J Syst Evol Microbiol63:2859–2864 [CrossRef][PubMed]
    [Google Scholar]
  22. Jeon C. O., Lim J. M., Park D. J., Kim C. J.. 2005; Salinimonas chungwhensis gen. nov., sp. nov., a moderately halophilic bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol55:239–243 [CrossRef][PubMed]
    [Google Scholar]
  23. Jin H. M., Jeong H. I., Jeon C. O.. 2015; Aliiglaciecola aliphaticivorans sp. nov., an aliphatic hydrocarbon-degrading bacterium, isolated from a sea-tidal flat and emended description of the genus Aliiglaciecola Jean et al. 2013. Int J Syst Evol Microbiol65:1550–1555 [CrossRef][PubMed]
    [Google Scholar]
  24. Kates M.. 1986; Techniques of Lipidology, 2nd edn. Amsterdam: Elsevier;
    [Google Scholar]
  25. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  26. Kimura M.. 1983; Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [CrossRef]
    [Google Scholar]
  27. Komagata K., Suzuki K.. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol19:161–207 [CrossRef]
    [Google Scholar]
  28. Kurahashi M., Yokota A.. 2004; Agarivorans albus gen. nov., sp. nov., a γ-proteobacterium isolated from marine animals. Int J Syst Evol Microbiol54:693–697 [CrossRef][PubMed]
    [Google Scholar]
  29. Lai Q., Yuan J., Wang B., Sun F., Qiao N., Zheng T., Shao Z.. 2009; Bowmanella pacifica sp. nov., isolated from a pyrene-degrading consortium. Int J Syst Evol Microbiol59:1579–1582 [CrossRef][PubMed]
    [Google Scholar]
  30. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A., other authors. 2007; Clustal w and Clustal x version 2.0. Bioinformatics23:2947–2948 [CrossRef][PubMed]
    [Google Scholar]
  31. Lim J. M., Jeon C. O., Lee J. C., Song S. M., Kim K. Y., Kim C. J.. 2006; Marinimicrobium koreense gen. nov., sp. nov. and Marinimicrobium agarilyticum sp. nov., novel moderately halotolerant bacteria isolated from tidal flat sediment in Korea. Int J Syst Evol Microbiol56:653–657 [CrossRef][PubMed]
    [Google Scholar]
  32. López-Pérez M., Rodriguez-Valera F.. 2014; The family Alteromonadaceae . In The Prokaryotes pp69–92Edited by Rosenberg E., DeLong E., Lory S., Stackebrandt E., Thompson F.. Berlin Heidelberg: Springer;
    [Google Scholar]
  33. Marmur J., Doty P.. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol5:109–118 [CrossRef][PubMed]
    [Google Scholar]
  34. Martínez-Checa F., Béjar V., Llamas I., Del Moral A., Quesada E.. 2005; Alteromonas hispanica sp. nov., a polyunsaturated-fatty-acid-producing, halophilic bacterium isolated from Fuente de Piedra, southern Spain. Int J Syst Evol Microbiol55:2385–2390 [CrossRef][PubMed]
    [Google Scholar]
  35. Matsuyama H., Hirabayashi T., Kasahara H., Minami H., Hoshino T., Yumoto I.. 2006; Glaciecola chathamensis sp. nov., a novel marine polysaccharide-producing bacterium. Int J Syst Evol Microbiol56:2883–2886 [CrossRef][PubMed]
    [Google Scholar]
  36. Matsuyama H., Minami H., Sakaki T., Kasahara H., Baba S., Ishimaru S., Hirota K., Yumoto I.. 2015; Alteromonas gracilis sp. nov., a marine polysaccharide-producing bacterium. Int J Syst Evol Microbiol65:1498–1503 [CrossRef][PubMed]
    [Google Scholar]
  37. Nokhal T.-H., Schlegel H. G.. 1983; Taxonomic study of Paracoccus denitrificans . Int J Syst Bacteriol33:26–37 [CrossRef]
    [Google Scholar]
  38. Park S., Yoon J. H.. 2013; Glaciecola aquimarina sp. nov., a gammaproteobacterium isolated from coastal seawater. Antonie van Leeuwenhoek103:1141–1148 [CrossRef][PubMed]
    [Google Scholar]
  39. Romanenko L. A., Zhukova N. V., Rohde M., Lysenko A. M., Mikhailov V. V., Stackebrandt E.. 2003; Glaciecola mesophila sp. nov., a novel marine agar-digesting bacterium. Int J Syst Evol Microbiol53:647–651 [CrossRef][PubMed]
    [Google Scholar]
  40. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  41. Shivaji S., Reddy G. S.. 2014; Phylogenetic analyses of the genus Glaciecola: emended description of the genus Glaciecola, transfer of Glaciecola mesophila, G. agarilytica, G. aquimarina, G. arctica, G. chathamensis, G. polaris and G. psychrophila to the genus Paraglaciecola gen. nov. as Paraglaciecola mesophila comb. nov., P. agarilytica comb. nov., P. aquimarina comb. nov., P. arctica comb. nov., P. chathamensis comb. nov., P. polaris comb. nov. and P. psychrophila comb. nov., and description of Paraglaciecola oceanifecundans sp. nov., isolated from the Southern Ocean. Int J Syst Evol Microbiol64:3264–3275 [CrossRef][PubMed]
    [Google Scholar]
  42. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  43. Tanaka N., Romanenko L. A., Frolova G. M., Mikhailov V. V.. 2010; Aestuariibacter litoralis sp. nov., isolated from a sandy sediment of the Sea of Japan. Int J Syst Evol Microbiol60:317–320 [CrossRef][PubMed]
    [Google Scholar]
  44. Teramoto M., Nishijima M.. 2014; Agaribacter marinus gen. nov., sp. nov., an agar-degrading bacterium from surface seawater. Int J Syst Evol Microbiol64:2416–2423 [CrossRef][PubMed]
    [Google Scholar]
  45. Urios L., Intertaglia L., Lesongeur F., Lebaron P.. 2008; Haliea salexigens gen. nov., sp. nov., a member of the Gammaproteobacteria from the Mediterranean Sea. Int J Syst Evol Microbiol58:1233–1237 [CrossRef][PubMed]
    [Google Scholar]
  46. Van Trappen S., Tan T. L., Yang J., Mergaert J., Swings J.. 2004a; Glaciecola polaris sp. nov., a novel budding and prosthecate bacterium from the Arctic Ocean, and emended description of the genus Glaciecola . Int J Syst Evol Microbiol54:1765–1771 [CrossRef][PubMed]
    [Google Scholar]
  47. Van Trappen S., Tan T. L., Yang J., Mergaert J., Swings J.. 2004b; Alteromonas stellipolaris sp. nov., a novel, budding, prosthecate bacterium from Antarctic seas, and emended description of the genus Alteromonas . Int J Syst Evol Microbiol54:1157–1163 [CrossRef][PubMed]
    [Google Scholar]
  48. Vandecandelaere I., Nercessian O., Segaert E., Achouak W., Mollica A., Faimali M., De Vos P., Vandamme P.. 2008; Alteromonas genovensis sp. nov., isolated from a marine electroactive biofilm and emended description of Alteromonas macleodii Baumann et al. 1972 (approved lists 1980). Int J Syst Evol Microbiol58:2589–2596 [CrossRef][PubMed]
    [Google Scholar]
  49. Vogel B. F., Venkateswaran K., Christensen H., Falsen E., Christiansen G., Gram L.. 2000; Polyphasic taxonomic approach in the description of Alishewanella fetalis gen. nov., sp. nov., isolated from a human foetus. Int J Syst Evol Microbiol50:1133–1142 [CrossRef][PubMed]
    [Google Scholar]
  50. Wang Y., Wang H., Liu J., Lai Q., Shao Z., Austin B., Zhang X.-H.. 2010; Aestuariibacter aggregatus sp. nov., a moderately halophilic bacterium isolated from seawater of the Yellow Sea. FEMS Microbiol Lett309:48–54[PubMed]
    [Google Scholar]
  51. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J.. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol173:697–703[PubMed]
    [Google Scholar]
  52. Yi H., Bae K. S., Chun J.. 2004; Aestuariibacter salexigens gen. nov., sp. nov. and Aestuariibacter halophilus sp. nov., isolated from tidal flat sediment, and emended description of Alteromonas macleodii . Int J Syst Evol Microbiol54:571–576 [CrossRef][PubMed]
    [Google Scholar]
  53. Yong J. J., Park S. J., Kim H. J., Rhee S. K.. 2007; Glaciecola agarilytica sp. nov., an agar-digesting marine bacterium from the East Sea, Korea. Int J Syst Evol Microbiol57:951–953 [CrossRef][PubMed]
    [Google Scholar]
  54. Yoon J. H., Kim I. G., Kang K. H., Oh T. K., Park Y. H.. 2003; Alteromonas marina sp. nov., isolated from sea water of the East Sea in Korea. Int J Syst Evol Microbiol53:1625–1630 [CrossRef][PubMed]
    [Google Scholar]
  55. Yoon J. H., Yeo S. H., Oh T. K., Park Y. H.. 2004; Alteromonas litorea sp. nov., a slightly halophilic bacterium isolated from an intertidal sediment of the Yellow Sea in Korea. Int J Syst Evol Microbiol54:1197–1201 [CrossRef][PubMed]
    [Google Scholar]
  56. Yoon J. H., Kang S. J., Lee S. Y.. 2012; Salinimonas lutimaris sp. nov., a polysaccharide-degrading bacterium isolated from a tidal flat. Antonie van Leeuwenhoek101:803–810 [CrossRef][PubMed]
    [Google Scholar]
  57. Zhang D.-C., Yu Y., Chen B., Wang H.-X., Liu H.-C., Dong X.-Z., Zhou P.-J.. 2006; Glaciecola psychrophila sp. nov., a novel psychrophilic bacterium isolated from the Arctic. Int J Syst Evol Microbiol56:2867–2869 [CrossRef][PubMed]
    [Google Scholar]
  58. Zhang Y.-J., Zhang X.-Y., Mi Z.-H., Chen C.-X., Gao Z.-M., Chen X.-L., Yu Y., Chen B., Zhang Y.-Z.. 2011; Glaciecola arctica sp. nov., isolated from Arctic marine sediment. Int J Syst Evol Microbiol61:2338–2341 [CrossRef][PubMed]
    [Google Scholar]
  59. Zhong Z.-P., Liu Y., Liu H.-C., Wang F., Zhou Y.-G., Liu Z.-P.. 2014; Roseibium aquae sp. nov., isolated from a saline lake. Int J Syst Evol Microbiol64:2812–2818[PubMed][CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000735
Loading
/content/journal/ijsem/10.1099/ijsem.0.000735
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error